{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T20:22:25Z","timestamp":1793391745485,"version":"4.5.0"},"reference-count":67,"publisher":"BMJ","issue":"4","license":[{"start":{"date-parts":[[2025,4,19]],"date-time":"2025-04-19T00:00:00Z","timestamp":1745020800000},"content-version":"unspecified","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006386","name":"Adenoid Cystic Carcinoma Research Foundation","doi-asserted-by":"publisher","award":["2024A008254"],"award-info":[{"award-number":["2024A008254"]}],"id":[{"id":"10.13039\/100006386","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100011957","name":"Takeda Oncology","doi-asserted-by":"publisher","award":["NCT04420884"],"award-info":[{"award-number":["NCT04420884"]}],"id":[{"id":"10.13039\/100011957","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["J Immunother Cancer"],"accepted":{"date-parts":[[2025,4,3]]},"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Adenoid cystic carcinoma (ACC) is a rare, but lethal cancer with low response rates to systemic therapies, such as cytotoxic chemotherapy and immune-checkpoint inhibitors (ICIs). Despite extensive clinical trials, no effective treatments for patients with recurrent or metastatic ACC are available, and ACC mortality rates remain poor.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We employed automated multiplex immunofluorescence (mIF), single-cell RNA sequencing (scRNA-seq) Gene Expression analysis, RNA in-situ hybridization, and spatial transcriptomics analysis to characterize the immune landscape of ACC tumors, ACC metastasis, and normal tissues from regions where ACCs arise. Based on results from these studies, we treated freshly resected ACCs with interferon-\u03b3 or a stimulator of the interferon genes (STING) agonist in vitro. Additionally, we included one patient with ACC in a phase 1 clinical study of a novel STING agonist (dazostinag) plus pembrolizumab.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The mIF analysis revealed that ACC tumors are immunologically \u201ccold\u201d, with few tumor-infiltrating T-lymphocytes and low programmed death-ligand 1 (PD-L1) expression. The most striking finding was a very low beta-2-microglobulin (B2M) expression in nearly all ACCs, with only focal expression found in some ACC metastases. mIF and RNA sequencing analyses of normal salivary gland and breast tissues revealed a p63+, NFIB+, basal duct cell population, with similarly low B2M\/human leukocyte antigen (HLA) class I expression. Spatial transcriptomics analysis of the focally B2M-positive ACC metastases uncovered the genetic pathway driving upregulation of B2M, an interferon-\u03b3 program mediating the reintroduction of HLA-I\/B2M; the significantly upregulated genes included\n                      <jats:italic>IRF1, GBP1,<\/jats:italic>\n                      and\n                      <jats:italic>TAP1<\/jats:italic>\n                      . On short-term treatment of primary ACC tissues in vitro with interferon-\u03b3 or a STING agonist, we observed strongly upregulated HLA class I\/B2M expression. Moreover, treatment of a patient with recurrent, metastatic breast ACC with a STING agonist and pembrolizumab led to a partial response with a 70% tumor reduction.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Low B2M\/HLA class I expression may explain why ACCs are immunologically cold and the lack of response to ICIs. Our findings suggest that the normal cell of ACC origin exists in a B2M\/HLA-class I low state, and that pharmacologic manipulation with immune activators, such as STING agonists, can restore HLA\/B2M in ACCs, as supported by the promising response observed in a patient with metastatic ACC. These findings indicate a potential path to urgently needed immunotherapies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/jitc-2024-011380","type":"journal-article","created":{"date-parts":[[2025,4,20]],"date-time":"2025-04-20T06:55:11Z","timestamp":1745132111000},"page":"e011380","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":13,"title":["Reversible downregulation of HLA class I in adenoid cystic carcinoma"],"prefix":"10.1136","volume":"13","clinical-trial-number":[{"clinical-trial-number":"nct04420884","registry":"10.18810\/clinical-trials-gov"},{"clinical-trial-number":"nct04420884","registry":"10.18810\/clinical-trials-gov"},{"clinical-trial-number":"nct04420884","registry":"10.18810\/clinical-trials-gov"}],"author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-7020-5794","authenticated-orcid":false,"given":"Annie","family":"Li","sequence":"first","affiliation":[{"name":"Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bianca L","family":"Gonda","sequence":"additional","affiliation":[{"name":"Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8738-6258","authenticated-orcid":false,"given":"Elizabeth M","family":"Codd","sequence":"additional","affiliation":[{"name":"Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"von Paternos","sequence":"additional","affiliation":[{"name":"Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-1349-4145","authenticated-orcid":false,"given":"Dawn R","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus D","family":"Herrmann","sequence":"additional","affiliation":[{"name":"Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2684-3789","authenticated-orcid":false,"given":"Prinjali","family":"Kalyan","sequence":"additional","affiliation":[{"name":"Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-8347-4940","authenticated-orcid":false,"given":"Samantha E","family":"Flynn","sequence":"additional","affiliation":[{"name":"Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1038-6328","authenticated-orcid":false,"given":"Thuc Q","family":"Dzu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengzhuo","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia J","family":"Mendel","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Head and Neck Surgery, Massachusetts Eye and Ear, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia C","family":"Thierauf","sequence":"additional","affiliation":[{"name":"Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter M","family":"Sadow","sequence":"additional","affiliation":[{"name":"Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Denize","sequence":"additional","affiliation":[{"name":"Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diane","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0119-3903","authenticated-orcid":false,"given":"Florian J","family":"Fintelmann","sequence":"additional","affiliation":[{"name":"Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, Masachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo Anne","family":"Fordham","sequence":"additional","affiliation":[{"name":"Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3418-2220","authenticated-orcid":false,"given":"Ross D","family":"Merkin","sequence":"additional","affiliation":[{"name":"Department of Medicine, Hematology-Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atul K","family":"Bhan","sequence":"additional","affiliation":[{"name":"Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Chung","family":"Huang","sequence":"additional","affiliation":[{"name":"Oncology Development, Takeda Pharmaceuticals, Development Center Americas Inc, Lexington, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey","family":"Raizer","sequence":"additional","affiliation":[{"name":"Oncology Development, Takeda Pharmaceuticals, Development Center Americas Inc, Lexington, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William C","family":"Faquin","sequence":"additional","affiliation":[{"name":"Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel L","family":"Faden","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Head and Neck Surgery, Massachusetts Eye and Ear, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Medicine, Hematology-Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1052-0734","authenticated-orcid":false,"given":"Jong Chul","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Medicine, Hematology-Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lori J","family":"Wirth","sequence":"additional","affiliation":[{"name":"Department of Medicine, Hematology-Oncology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan T","family":"Kaluziak","sequence":"additional","affiliation":[{"name":"Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. John","family":"Iafrate","sequence":"additional","affiliation":[{"name":"Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2025,4,20]]},"reference":[{"key":"2026052108353594000_13.4.e011380.1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9610(74)90265-7"},{"key":"2026052108353594000_13.4.e011380.2","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(82)80052-X"},{"key":"2026052108353594000_13.4.e011380.3","doi-asserted-by":"crossref","DOI":"10.1186\/bcr2613","article-title":"Adenoid cystic carcinoma of the breast in the United States (1977 to 2006): a population-based cohort study","volume":"12","author":"Ghabach","year":"2010","journal-title":"Breast Cancer Res"},{"key":"2026052108353594000_13.4.e011380.4","doi-asserted-by":"crossref","first-page":"152","DOI":"10.3857\/roj.2021.00122","article-title":"Lacrimal gland adenoid cystic carcinoma: report of an unusual case with literature review","volume":"39","author":"Benali","year":"2021","journal-title":"Radiat Oncol J"},{"key":"2026052108353594000_13.4.e011380.5","doi-asserted-by":"crossref","first-page":"4496","DOI":"10.1002\/cam4.5181","article-title":"The GAL\/GALR2 axis promotes the perineural invasion of salivary adenoid cystic carcinoma via epithelial-to-mesenchymal transition","volume":"12","author":"Wang","year":"2023","journal-title":"Cancer Med"},{"key":"2026052108353594000_13.4.e011380.6","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.1002\/cam4.3707","article-title":"Multicentre clinicopathological study of adenoid cystic carcinoma: A report of 296 cases","volume":"10","author":"Huang","year":"2021","journal-title":"Cancer Med"},{"key":"2026052108353594000_13.4.e011380.7","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9610(97)00153-0"},{"key":"2026052108353594000_13.4.e011380.8","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1016\/j.ijom.2013.04.006","article-title":"Clinicopathological study of distant metastases of salivary adenoid cystic carcinoma","volume":"42","author":"Gao","year":"2013","journal-title":"Int J Oral Maxillofac Surg"},{"key":"2026052108353594000_13.4.e011380.9","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.125.2.149"},{"key":"2026052108353594000_13.4.e011380.10","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9610(05)80721-4"},{"key":"2026052108353594000_13.4.e011380.11","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjoto.2016.11.014"},{"key":"2026052108353594000_13.4.e011380.12","doi-asserted-by":"publisher","DOI":"10.21873\/anticanres.11659"},{"key":"2026052108353594000_13.4.e011380.13","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.21.00449"},{"key":"2026052108353594000_13.4.e011380.14","unstructured":"NCCN . National comprehensive cancer network clinical practice guidelines in oncology: head and neck tumors version 3.2024. 2024. Available: https:\/\/www.nccn.org\/professionals\/physician_gls\/pdf\/head-and-neck.pdf [Accessed 5 Apr 2024]."},{"key":"2026052108353594000_13.4.e011380.15","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-15-2867-T"},{"key":"2026052108353594000_13.4.e011380.16","doi-asserted-by":"crossref","DOI":"10.3390\/cancers14153691","article-title":"Rearrangements, Expression, and Clinical Significance of MYB and MYBL1 in Adenoid Cystic Carcinoma: A Multi-Institutional Study","volume":"14","author":"Persson","year":"2022","journal-title":"Cancers (Basel)"},{"key":"2026052108353594000_13.4.e011380.17","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0909114106"},{"key":"2026052108353594000_13.4.e011380.18","doi-asserted-by":"publisher","DOI":"10.1634\/theoncologist.2018-0515"},{"key":"2026052108353594000_13.4.e011380.19","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-20-1192"},{"key":"2026052108353594000_13.4.e011380.20","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdw287"},{"key":"2026052108353594000_13.4.e011380.21","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.18.01859"},{"key":"2026052108353594000_13.4.e011380.22","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijrobp.2020.08.018"},{"key":"2026052108353594000_13.4.e011380.23","doi-asserted-by":"crossref","DOI":"10.3390\/cancers14040992","article-title":"A Contemporary Review of Molecular Therapeutic Targets for Adenoid Cystic Carcinoma","volume":"14","author":"Miller","year":"2022","journal-title":"Cancers (Basel)"},{"key":"2026052108353594000_13.4.e011380.24","doi-asserted-by":"crossref","DOI":"10.3389\/fgene.2023.1144945","article-title":"Transcriptomic insights into adenoid cystic carcinoma via RNA sequencing","volume":"14","author":"Tang","year":"2023","journal-title":"Front Genet"},{"key":"2026052108353594000_13.4.e011380.25","doi-asserted-by":"crossref","first-page":"212","DOI":"10.4103\/ijmpo.ijmpo_217_17","article-title":"Eosinophil density in common benign and malignant salivary gland tumors with Congo red staining","volume":"40","author":"Seifi","year":"2019","journal-title":"Indian J Med Paediatr Oncol"},{"key":"2026052108353594000_13.4.e011380.26","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.oraloncology.2018.11.028","article-title":"Immune microenvironment and evasion mechanisms in adenoid cystic carcinomas of salivary glands","volume":"88","author":"Mosconi","year":"2019","journal-title":"Oral Oncol"},{"key":"2026052108353594000_13.4.e011380.27","doi-asserted-by":"publisher","DOI":"10.1158\/2326-6066.CIR-16-0031"},{"key":"2026052108353594000_13.4.e011380.28","doi-asserted-by":"crossref","DOI":"10.3389\/fimmu.2019.00168","article-title":"Cold Tumors: A Therapeutic Challenge for Immunotherapy","volume":"10","author":"Bonaventura","year":"2019","journal-title":"Front Immunol"},{"key":"2026052108353594000_13.4.e011380.29","doi-asserted-by":"publisher","DOI":"10.1097\/iop.0000000000001585"},{"key":"2026052108353594000_13.4.e011380.30","doi-asserted-by":"publisher","DOI":"10.1007\/s00405-023-07884-3"},{"key":"2026052108353594000_13.4.e011380.31","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-019-49215-9","article-title":"The expression of PD-L1 in salivary gland carcinomas","volume":"9","author":"Vital","year":"2019","journal-title":"Sci Rep"},{"key":"2026052108353594000_13.4.e011380.32","doi-asserted-by":"crossref","first-page":"2983","DOI":"10.2147\/OTT.S134589","article-title":"Overexpression of PD-L2 is associated with shorter relapse-free survival in patients with malignant salivary gland tumors","volume":"10","author":"Chang","year":"2017","journal-title":"Onco Targets Ther"},{"key":"2026052108353594000_13.4.e011380.33","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2021.06.008"},{"key":"2026052108353594000_13.4.e011380.34","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-012-1321-6"},{"key":"2026052108353594000_13.4.e011380.35","unstructured":"Sondka Z , Dhir NB , Carvalho-Silva D , et al . Data from: cosmic: a curated database of somatic variants and clinical data for cancer cosmic. 2024. Available: https:\/\/cancer.sanger.ac.uk\/census"},{"key":"2026052108353594000_13.4.e011380.36","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-023-00957-7"},{"key":"2026052108353594000_13.4.e011380.37","doi-asserted-by":"crossref","unstructured":"Zhou MJ , Yang JJ , Ma TY , et al . Data from: Increased retinoic acid signaling decreases lung metastasis in salivary adenoid cystic carcinoma by inhibiting the noncanonical Notch1 pathway NCBI GEO Gene Expression Omnibus. 2023. Available: https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE217084","DOI":"10.1038\/s12276-023-00957-7"},{"key":"2026052108353594000_13.4.e011380.38","doi-asserted-by":"crossref","DOI":"10.1126\/sciadv.abh2169","article-title":"A single-cell type transcriptomics map of human tissues","volume":"7","author":"Karlsson","year":"2021","journal-title":"Sci Adv"},{"key":"2026052108353594000_13.4.e011380.39","unstructured":"Tabula Sapiens C , Jones RC , Karkanias J , et al . Data from: The Tabula Sapiens: a multiple-organ, single-cell transcriptomic atlas of humans Human Protein Atlas - Single cell type data. 2022 Available: https:\/\/www.proteinatlas.org\/humanproteome\/single+cell\/single+cell+type\/data#datasets"},{"key":"2026052108353594000_13.4.e011380.40","doi-asserted-by":"publisher","DOI":"10.1017\/S0022215100110552"},{"key":"2026052108353594000_13.4.e011380.41","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2009.07.016"},{"key":"2026052108353594000_13.4.e011380.42","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2005.07.007"},{"key":"2026052108353594000_13.4.e011380.43","doi-asserted-by":"crossref","first-page":"S678","DOI":"10.1016\/j.annonc.2024.08.1055","article-title":"996MO Dazostinag (TAK-676) alone and in combination with pembrolizumab (pembro) in patients (pts) with advanced\/metastatic solid tumors: data from phase I dose escalation","volume":"35","author":"Luke","year":"2024","journal-title":"Ann Oncol"},{"key":"2026052108353594000_13.4.e011380.44","doi-asserted-by":"publisher","DOI":"10.1200\/jco.22.02221"},{"key":"2026052108353594000_13.4.e011380.45","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-19-3758"},{"key":"2026052108353594000_13.4.e011380.46","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.Ccr-23-0514"},{"key":"2026052108353594000_13.4.e011380.47","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.10.2777"},{"key":"2026052108353594000_13.4.e011380.48","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-20-0672"},{"key":"2026052108353594000_13.4.e011380.49","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00207-08"},{"key":"2026052108353594000_13.4.e011380.50","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-017-01062-w","article-title":"Resistance to checkpoint blockade therapy through inactivation of antigen presentation","volume":"8","author":"Sade-Feldman","year":"2017","journal-title":"Nat Commun"},{"key":"2026052108353594000_13.4.e011380.51","doi-asserted-by":"publisher","DOI":"10.1172\/JCI67201"},{"key":"2026052108353594000_13.4.e011380.52","doi-asserted-by":"crossref","DOI":"10.3389\/fcell.2021.740574","article-title":"Research Progress, Challenges, and Breakthroughs of Organoids as Disease Models","volume":"9","author":"Huang","year":"2021","journal-title":"Front Cell Dev Biol"},{"key":"2026052108353594000_13.4.e011380.53","doi-asserted-by":"crossref","DOI":"10.1038\/s41536-020-00115-x","article-title":"The evolving definition of salivary gland stem cells","volume":"6","author":"Rocchi","year":"2021","journal-title":"NPJ Regen Med"},{"key":"2026052108353594000_13.4.e011380.54","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1098"},{"key":"2026052108353594000_13.4.e011380.55","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-023-02518-x"},{"key":"2026052108353594000_13.4.e011380.56","doi-asserted-by":"publisher","DOI":"10.1158\/2159-8290.CD-20-0913"},{"key":"2026052108353594000_13.4.e011380.57","doi-asserted-by":"crossref","DOI":"10.1038\/s41467-023-37224-2","article-title":"Interoperable slide microscopy viewer and annotation tool for imaging data science and computational pathology","volume":"14","author":"Gorman","year":"2023","journal-title":"Nat Commun"},{"key":"2026052108353594000_13.4.e011380.58","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-020-01018-x"},{"key":"2026052108353594000_13.4.e011380.59","doi-asserted-by":"crossref","unstructured":"Stringer C , Pachitariu M . Cellpose3: one-click image restoration for improved cellular segmentation. Bioinformatics [Preprint]. doi:10.1101\/2024.02.10.579780","DOI":"10.1101\/2024.02.10.579780"},{"key":"2026052108353594000_13.4.e011380.60","unstructured":"Burri OS , Fehlmann T . BIOP\/qupath-extension-cellpose: parallel object reader, normalization bugfix gpu disable option and more (v0.9.3). 2024."},{"key":"2026052108353594000_13.4.e011380.61","doi-asserted-by":"crossref","DOI":"10.1038\/s41598-017-17204-5","article-title":"QuPath: Open source software for digital pathology image analysis","volume":"7","author":"Bankhead","year":"2017","journal-title":"Sci Rep"},{"key":"2026052108353594000_13.4.e011380.62","unstructured":"Nguyen D . Quantifying chromogen intensity in immunohistochemistry via reciprocal intensity. 2013. Available: https:\/\/assets-eu.researchsquare.com\/files\/nprot-2931\/v1\/ff8f61e8-c9d9-44ea-94b2-c04c8f25b243.pdf?c=1631825057"},{"key":"2026052108353594000_13.4.e011380.63","doi-asserted-by":"publisher","DOI":"10.1002\/pros.24019"},{"key":"2026052108353594000_13.4.e011380.64","unstructured":"Krueger F . TrimGalore: a wrapper tool around cutadapt and fastQC to consistently apply quality and adapter trimming to FastQ files. Babraham Bioinformatics 2015."},{"key":"2026052108353594000_13.4.e011380.65","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa1070"},{"key":"2026052108353594000_13.4.e011380.66","doi-asserted-by":"publisher","DOI":"10.1038\/35057062"},{"key":"2026052108353594000_13.4.e011380.67","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts635"}],"container-title":["Journal for ImmunoTherapy of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jitc-2024-011380","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T15:36:05Z","timestamp":1779377765000},"score":1,"resource":{"primary":{"URL":"https:\/\/jitc.bmj.com\/lookup\/doi\/10.1136\/jitc-2024-011380"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4]]},"references-count":67,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2025,4,20]]},"published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1136\/jitc-2024-011380"],"URL":"https:\/\/doi.org\/10.1136\/jitc-2024-011380","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2024.09.03.610529","asserted-by":"object"}]},"ISSN":["2051-1426"],"issn-type":[{"value":"2051-1426","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4]]}}}