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However, the molecular mechanisms driving this phenotype remain incompletely understood.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We integrated single-cell RNA sequencing and Xenium in situ spatial transcriptomics to analyze the immune microenvironment of five SCLC and four non-small cell lung cancer (NSCLC) samples. Multiplex immunofluorescence was used to validate cell types and gene expression in the same tissue specimens, and animal models were employed to verify the key mechanistic pathway.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      SCLC displayed a distinct immune landscape compared with NSCLC, with increased infiltration of C-X-C motif chemokine receptor 4 (CXCR4)\n                      <jats:sup>+<\/jats:sup>\n                      neutrophils (via neutrophil extracellular traps) and S100A8\n                      <jats:sup>+<\/jats:sup>\n                      monocytes (toward an M2-like phenotype), and reduced CD8\n                      <jats:sup>+<\/jats:sup>\n                      T-cell infiltration. Malignant epithelial cells in SCLC highly expressed CXCR4, regulated by transcription factors ISL LIM homeobox 1 and distal-less homeobox 5, which promoted immunosuppression. The C-X-C motif chemokine ligand 12 (CXCL12)\u2013CXCR4 axis mediated competitive inhibition, impairing T-cell recruitment while enhancing neutrophil accumulation. Monocytes in SCLC shifted toward an M2-like phenotype, weakening antigen presentation. Xenium spatial transcriptomics confirmed colocalization of CXCR4\n                      <jats:sup>+<\/jats:sup>\n                      neutrophils and S100A8\n                      <jats:sup>+<\/jats:sup>\n                      monocytes with tumor cells at the tumor-normal interface, while CD8\n                      <jats:sup>+<\/jats:sup>\n                      T cells were spatially segregated. In vivo experiments showed that CXCR4 inhibition reduced SCLC tumor growth, decreased immunosuppressive cell infiltration, and enhanced CD8\n                      <jats:sup>+<\/jats:sup>\n                      T-cell accumulation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The CXCL12\u2013CXCR4 axis, together with immunosuppressive CXCR4\n                      <jats:sup>+<\/jats:sup>\n                      neutrophils and S100A8\n                      <jats:sup>+<\/jats:sup>\n                      monocytes, is a key driver of the immune-desert phenotype in SCLC. Targeting this axis holds promise as a therapeutic strategy to remodel the immunosuppressive TME and improve the efficacy of immunotherapy for SCLC.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/jitc-2025-013867","type":"journal-article","created":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T14:05:15Z","timestamp":1776348315000},"page":"e013867","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":3,"title":["Single-cell and spatial transcriptomics reveal that the CXCL12\u2013CXCR4 axis drives the immune-desert phenotype in small cell lung cancer by recruiting immunosuppressive CXCR4\n                    <sup>+<\/sup>\n                    neutrophils and S100A8\n                    <sup>+<\/sup>\n                    monocytes"],"prefix":"10.1136","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1606-7528","authenticated-orcid":false,"given":"Peng","family":"Zeng","sequence":"first","affiliation":[{"name":"Department of Oncology, Southeast University Zhongda Hospital, Nanjing, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai-Feng","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Hepato-Pancreatico-Biliary Surgery, Southeast University Zhongda Hospital, Nanjing, Jiangsu, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Bin","family":"Shu","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery, Nanchang University Second Affiliated Hospital, Nanchang, Jiangxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Southeast University School of Medicine, Nanjing, Jiangsu, 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