{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T22:10:36Z","timestamp":1760739036913,"version":"build-2065373602"},"reference-count":11,"publisher":"BMJ","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Med Genet"],"published-print":{"date-parts":[[1979,8]]},"abstract":"<jats:p>An abnormal cell population, 45,X, appeared in 3 of 4 cell lines established from an amniotic fluid specimen obtained from a normal mid-trimester pregnancy. Two of the cell lines were subjected to repeated chromosome analyses until VII passage. The abnormal cells were suppressed after repeated trypsinisations; simultaneously, fibroblast-like cells outgrew the cultures, which were previously predominated by epithelial-like cells. Polyploidy was found in 0 to 12% of the cells, the highest level existing in the early passages. The question of whether chromosomally abnormal cells present in primary cultures and the early subcultures reflect the karyotype of the fetus is discussed.<\/jats:p>","DOI":"10.1136\/jmg.16.4.267","type":"journal-article","created":{"date-parts":[[2008,11,10]],"date-time":"2008-11-10T14:50:51Z","timestamp":1226328651000},"page":"267-269","source":"Crossref","is-referenced-by-count":1,"title":["46,XY\/45,X mosaicism in an amniotic fluid cell culture: suppression of abnormal cell line after subcultivation."],"prefix":"10.1136","volume":"16","author":[{"given":"L","family":"Hasholt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1979,8,1]]},"reference":[{"key":"ref_1","first-page":"103","article-title":"A case of 46, XX\/47, XX, + G mosaicism in amniotic fluid not confirmed in the fetus","volume":"13","author":"Aula, P.; von Koskull, H.; Ryynalnen, M.","year":"1978","unstructured":"Aula, P., von Koskull, H. and Ryynalnen, M. (1978). A case of 46,XX\/47,XX, + G mosaicism in amniotic fluid not confirmed in the fetus. Clinical Genetics, 13, 103.","journal-title":"Clinical Genetics"},{"key":"ref_2","first-page":"409","article-title":"In vitro cultivation of cells from aneuploid human embryos. Initiation of cell lines and longevity of the cultures","volume-title":"Vitro","volume":"11","author":"Boue, A.; Boue, J.; Cure, S.; Deluchat, C.; Perraudin, N.","year":"1975","unstructured":"Boue, A., Boue, J., Cure, S., Deluchat, C., and Perraudin, N. (1975). In vitro cultivation of cells from aneuploid human embryos. Initiation of cell lines and longevity of the cultures. In Vitro, 11, 409-413."},{"key":"ref_3","first-page":"38","article-title":"Establishment and genetic stability of human amnion cell lines","volume":"24","author":"Conover, J.H.; Hirschhorn, K.","year":"1971","unstructured":"Conover, J. H., and Hirschhorn, K. (1971). Establishment and genetic stability of human amnion cell lines. Laboratory Investigation, 24, 38-40.","journal-title":"Laboratory Investigation"},{"key":"ref_4","first-page":"639","article-title":"Karyology of primary human fetal cell cultures","volume-title":"Vitro","volume":"12","author":"Earley, E.M.; Finley, S.C.; Finley, W.H.; Hsu, L.Y.F.; Kim, H.J.; Petricciani, J.C.; Vinson, P.C.","year":"1976","unstructured":"Earley, E. M., Finley, S. C., Finley, W. H., Hsu, L. Y. F., Kim, H. J., Petricciani, J. C., and Vinson, P. C. (1976). Karyology of primary human fetal cell cultures. In Vitro, 12, 639-641."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"294","DOI":"10.1111\/j.1399-0004.1974.tb02090.x","article-title":"Early prenatal diagnosis; a study of biopsy techniques and cell culturing from extraembryonic membranes","volume":"6","author":"Hahnemann, N.","year":"1974","unstructured":"Hahnemann, N. (1974). Early prenatal diagnosis; a study of biopsy techniques and cell culturing from extraembryonic membranes. 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K., Hausknecht, R., and Hirschhorn, K. (1976). Prenatal diagnosis of 45,X\/46,XY mosaicism with postnatal confirmation in a phenotypically normal male infant. Clinical Genetics, 10, 232-238.","journal-title":"Clinical Genetics"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1016\/S0140-6736(70)90262-X","article-title":"Tetraploidy in cells cultured from amniotic fluid","volume":"1","author":"Kohn, G.; Robinson, A.","year":"1970","unstructured":"Kohn, G., and Robinson, A. (1970). Tetraploidy in cells cultured from amniotic fluid. Lancet, 1, 778.","journal-title":"Lancet"},{"key":"ref_9","first-page":"367","article-title":"Prenatal diagnosis of chromosomal mosaicism","volume":"88","author":"Milunsky, A.; Atkins, L.","year":"1976","unstructured":"Milunsky, A., and Atkins, L. (1976). Prenatal diagnosis of chromosomal mosaicism. Journal ofPediatrics, 88, 367-368.","journal-title":"Journal ofPediatrics"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1111\/j.1399-0004.1978.tb04245.x","article-title":"Amniotic fluid cell mosaicism for presumptive trisomy 20","volume":"13","author":"Rodriguez, M.L.; Luthy, D.; Hall, J.G.; Norwood, T.H.; Hoehn, H.","year":"1978","unstructured":"Rodriguez, M. L., Luthy, D., Hall, J. G., Norwood, T. H., and Hoehn, H. (1978). Amniotic fluid cell mosaicism for presumptive trisomy 20. Clinical Genetics, 13, 164-168.","journal-title":"Clinical Genetics"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1111\/j.1399-0004.1975.tb00348.x","article-title":"Chromosomal mosaicism in amniotic fluid cell cultures","volume":"7","author":"Sutherland, G.R.; Bowser-Riley, S.M.; Bain, A.D.","year":"1975","unstructured":"Sutherland, G. R., Bowser-Riley, S. M., and Bain, A. D. (1975). Chromosomal mosaicism in amniotic fluid cell cultures. 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