{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T04:11:06Z","timestamp":1759723866984,"version":"build-2065373602"},"reference-count":8,"publisher":"BMJ","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Med Genet"],"published-print":{"date-parts":[[1988,9]]},"abstract":"<jats:p>An immunoassay based on a monoclonal antibody specific for acetylcholinesterase (AChE) was used prospectively over a two year period to screen second trimester amniotic fluids for fetal abnormalities. All 26 cases of spina bifida, three of which had normal alphafetoprotein (AFP) concentrations and ultrasound scans, were detected by the immunoassay. Four clear fluids, with abnormal AFP concentrations, had normal AChE titres and yielded normal outcomes. Some difficulties were encountered with both the AChE immunoassay and polyacrylamide gel testing when amniotic fluids were contaminated with old blood. However, the quantitative nature of the AChE immunoassay and its independence of operator experience make it an ideal adjunct to AFP assay for routine screening of amniotic fluids.<\/jats:p>","DOI":"10.1136\/jmg.25.9.606","type":"journal-article","created":{"date-parts":[[2008,11,10]],"date-time":"2008-11-10T15:11:11Z","timestamp":1226329871000},"page":"606-608","source":"Crossref","is-referenced-by-count":6,"title":["Prospective prenatal screening for fetal abnormalities using a quantitative immunoassay for acetylcholinesterase."],"prefix":"10.1136","volume":"25","author":[{"given":"D J","family":"Brock","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Barron","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[1988,9,1]]},"reference":[{"key":"ref_1","first-page":"685","article-title":"Second Report of the UK Collaborative Study on Alphafetoprotein in relation to Neural Tube Defects. Amniotic fluid alpha-fetoprotein measurement in antenatal diagnosis of anencephaly and open spina bifida in early pregnancy","volume":"i:","year":"1979","unstructured":"Second Report of the UK Collaborative Study on Alphafetoprotein in relation to Neural Tube Defects. Amniotic fluid alpha-fetoprotein measurement in antenatal diagnosis of anencephaly and open spina bifida in early pregnancy. Laticet 1979;i:685-8.","journal-title":"Laticet"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1007\/978-1-4684-5155-9_17","article-title":"Diagnosis of fetal abnormalities by ultrasound","volume-title":"disorders and the fetus","author":"Nicolaides, K.H.; Campbell, S.","year":"1986","unstructured":"Nicolaides KH, Campbell S. Diagnosis of fetal abnormalities by ultrasound. In: Milunsky A, ed. Genetic disorders and the fetus. 2nd ed. New York: Plenum Press, 1986:521-70.","edition":"2nd ed"},{"key":"ref_3","first-page":"\"453","article-title":"The prenatal diagnosis of neural tube and other congenital defects","volume":"ii:","author":"Milunsky, A.","year":"1986","unstructured":"Milunsky A. The prenatal diagnosis of neural tube and other congenital defects. In: Milunsky A. ed. Getelic disorders atnd the fetus. 2nd ed. New York: Plenum Press, 1986:453-519. 4Report of the Collaborative Acetylcholinesterase Study. Amniotic fluid acetylcholinesterase electrophoresis as a secondary test in the diagnosis of anencephaly and open spina bifida. Lancet 1981;ii:321-4.","edition":"2nd ed","journal-title":"Lancet; Getelic disorders atnd the fetus"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1016\/0009-8981(84)90139-6","article-title":"Quantitative estimation of the density ratios of cholinesterase bands in human amniotic fluids","volume":"138","author":"Peat, D.; Brock, D.J.H.","year":"1984","unstructured":"Peat D, Brock DJH. Quantitative estimation of the density ratios of cholinesterase bands in human amniotic fluids. Clin Chim Acta 1984;138:319-24.","journal-title":"Clin Chim Acta"},{"key":"ref_5","first-page":"5","article-title":"Prenatal diagnosis of neural-tube defects with a monoclonal antibody specific for acetylcholinesterase","volume":"1985","author":"Brock, D.J.H.; Barron, L.; van Heyningen, V.","unstructured":"Brock DJH, Barron L, van Heyningen V. Prenatal diagnosis of neural-tube defects with a monoclonal antibody specific for acetylcholinesterase. Lancet 1985:i:5-8.","journal-title":"Lancet"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/S0140-6736(72)91634-0","article-title":"Alphafetoprotein in the antenatal diagnosis of anencephaly and spina bifida","volume":"ii:","author":"Brock, D.J.H.; Sutcliffe, R.G.","year":"1972","unstructured":"Brock DJH, Sutcliffe RG. Alphafetoprotein in the antenatal diagnosis of anencephaly and spina bifida. Latncet 1972;ii: 197-9.","journal-title":"Latncet"},{"key":"ref_7","first-page":"135","article-title":"Gel electrophoresis of amniotic fluid acetylcholinesterase as an aid to the prenatal diagnosis of fetal defects","volume":"180","author":"Brock, D.J.H.; Hayward, C.","year":"1980","unstructured":"Brock DJH, Hayward C. Gel electrophoresis of amniotic fluid acetylcholinesterase as an aid to the prenatal diagnosis of fetal defects. Clin Chi,n Acta 1980;180:135-41.","journal-title":"Acta"},{"issue":"4","key":"ref_8","first-page":"329","article-title":"Predictive value of amniotic acetylcholinesterase analysis in the diagnosis of fetal abnormality in 37t)0 pregnancies","volume":"1984","author":"NM, Aitken D.A.Morrison; MA, Ferguson-Smith","unstructured":"Aitken DA. Morrison NM, Ferguson-Smith MA. Predictive value of amniotic acetylcholinesterase analysis in the diagnosis of fetal abnormality in 37t)0 pregnancies. Preniat Diagni 1984:4: 329-40).","journal-title":"Preniat Diagni"}],"container-title":["Journal of Medical Genetics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/jmg.25.9.606","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T03:53:04Z","timestamp":1759722784000},"score":1,"resource":{"primary":{"URL":"https:\/\/jmg.bmj.com\/lookup\/doi\/10.1136\/jmg.25.9.606"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,9]]},"references-count":8,"journal-issue":{"issue":"9","published-online":{"date-parts":[[1988,9,1]]},"published-print":{"date-parts":[[1988,9]]}},"alternative-id":["10.1136\/jmg.25.9.606"],"URL":"https:\/\/doi.org\/10.1136\/jmg.25.9.606","relation":{},"ISSN":["0022-2593","1468-6244"],"issn-type":[{"type":"print","value":"0022-2593"},{"type":"electronic","value":"1468-6244"}],"subject":[],"published":{"date-parts":[[1988,9]]}}}