{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T23:35:29Z","timestamp":1791934529395,"version":"4.3.4"},"reference-count":50,"publisher":"BMJ","issue":"2","license":[{"start":{"date-parts":[[2024,8,17]],"date-time":"2024-08-17T00:00:00Z","timestamp":1723852800000},"content-version":"unspecified","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Ministry of Science, Technology, and Innovation of Colombia","award":["844-2019."],"award-info":[{"award-number":["844-2019."]}]},{"name":"SAPIENCIA, postgraduate National Funds, Medell\u00edn, Colombia","award":["2022"],"award-info":[{"award-number":["2022"]}]},{"name":"University of Antioquia, support funds program, Medell\u00edn, Colombia","award":["2022"],"award-info":[{"award-number":["2022"]}]}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Lupus Sci Med"],"accepted":{"date-parts":[[2024,7,27]]},"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>This study aimed to identify the physicochemical and phenotypic characteristics of circulating Extracellular Vesicles (EVs) in the plasma of patients with SLE, with or without Lupus Nephritis (LN), and their potential utility as disease biomarkers.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>Plasma-circulating EVs were concentrated using differential centrifugation from adult female patients (n=38) who met the \u2018American College of Rheumatology\/European Alliance of Associations for Rheumatology 2019\u2019 criteria for SLE diagnosis with (LN) or without LN (nLN), confirmed by renal biopsy. Controls (n=18) were healthy volunteers matched by gender and similar age. The structure, size and Energy Dispersion Spectrum (EDS) of EVs were observed by electron microscopy. The surface charge and size distribution were evaluated using dynamic light scattering. The counts and phenotype of EVs from patients (SLE-EVs) and controls (Ctrl-EVs) were obtained using flow cytometry. Non-parametric statistical tests and exploratory analysis of multiple variables were performed. The discriminatory power of some variables as potential biomarkers of the disease was also evaluated.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Circulating EVs were heterogeneous in morphology and size, but SLE-EVs reached larger diameters than Ctrl-EVs (p&lt;0.0001). Small SLE-EVs and large SLE-EVs were increased compared with Ctrl-EV (p&lt;0.0001 and p&lt;0.05, respectively). Likewise, patients with SLE (LN or nLN) had higher concentrations of large EVs compared with controls (p&lt;0.001\u2009and p&lt;0.0001, respectively). SLE-EVs showed a different EDS (p&lt;0.001) and were less electronegative (p&lt;0.0001) than Ctrl-EVs. EV-CD45+, EV-CD14+ and EV-IgM+ were\u2009more frequent in patients with SLE compared with controls (p&lt;0.001, p&lt;0.05 and p&lt;0.001, respectively). The concentrations of large EVs and EV-IgM+ allowed better discrimination of patients from controls.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Plasma-circulating EVs from patients with SLE with and without nephritis are increased in peripheral blood and have different physicochemical properties than controls. Characteristics of EVs such as larger size and the presence of IgM on the surface could help discriminate patients from controls.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1136\/lupus-2024-001243","type":"journal-article","created":{"date-parts":[[2024,8,17]],"date-time":"2024-08-17T12:20:37Z","timestamp":1723897237000},"page":"e001243","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":5,"title":["Circulating extracellular vesicles in Systemic Lupus Erythematosus: physicochemical properties and phenotype"],"prefix":"10.1136","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6651-4720","authenticated-orcid":false,"given":"Paula X","family":"Losada","sequence":"first","affiliation":[{"name":"Universidad de Antioquia Grupo de Inmunolog\u00eda Celular e Inmunogen\u00e9tica, Medellin, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina","family":"Serrato","sequence":"additional","affiliation":[{"name":"Universidad de Antioquia Grupo de Inmunolog\u00eda Celular e Inmunogen\u00e9tica, Medellin, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Mar\u00eda","family":"Daza","sequence":"additional","affiliation":[{"name":"Universidad de Antioquia Grupo de Inmunolog\u00eda Celular e Inmunogen\u00e9tica, Medellin, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana","family":"Vanegas-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Grupo de Reumatolog\u00eda, Facultad de Medicina, Universidad de Antioquia, Medellin, Colombia"},{"name":"Hospital San Vicente de Pa\u00fal, Medellin, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos H","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Grupo de Reumatolog\u00eda, Facultad de Medicina, Universidad de Antioquia, Medellin, Colombia"},{"name":"Secci\u00f3n Reumatolog\u00eda, Hospital San Vicente de Pa\u00fal, Medellin, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7735-7954","authenticated-orcid":false,"given":"Daniel","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"ARTM\u00c9DICA IPS, Medell\u00edn, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Camilo","family":"Diaz","sequence":"additional","affiliation":[{"name":"ARTM\u00c9DICA IPS, Medell\u00edn, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo","family":"Pineda","sequence":"additional","affiliation":[{"name":"ARTM\u00c9DICA IPS, Medell\u00edn, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauricio","family":"Rojas Lopez","sequence":"additional","affiliation":[{"name":"Universidad de Antioquia Grupo de Inmunolog\u00eda Celular e Inmunogen\u00e9tica, Medellin, Colombia"},{"name":"Unidad de Citometr\u00eda de Flujo, Universidad de Antioquia, Medellin, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gloria","family":"V\u00e1squez","sequence":"additional","affiliation":[{"name":"Universidad de Antioquia Grupo de Inmunolog\u00eda Celular e Inmunogen\u00e9tica, Medellin, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2024,8,17]]},"reference":[{"key":"2025091512345076000_11.2.e001243.1","doi-asserted-by":"crossref","first-page":"999","DOI":"10.1136\/ard-2022-223741","article-title":"Pathogenesis of systemic lupus erythematosus: risks, mechanisms and therapeutic targets","volume":"82","author":"Crow","year":"2023","journal-title":"Ann Rheum Dis"},{"key":"2025091512345076000_11.2.e001243.2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-101921-042422"},{"key":"2025091512345076000_11.2.e001243.3","doi-asserted-by":"crossref","DOI":"10.1080\/20013078.2019.1648167","article-title":"Extracellular vesicles or exosomes? 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