{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T06:31:35Z","timestamp":1788157895151,"version":"build-2803163510"},"reference-count":50,"publisher":"BMJ","issue":"1","license":[{"start":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T00:00:00Z","timestamp":1750118400000},"content-version":"unspecified","delay-in-days":167,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"The Guangzhou Postdoctoral Scientific Research Startup Program","award":["No. 2023082213"],"award-info":[{"award-number":["No. 2023082213"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["Nos. 82360147, 82460148, and 82160137"],"award-info":[{"award-number":["Nos. 82360147, 82460148, and 82160137"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Lupus Sci Med"],"accepted":{"date-parts":[[2025,6,2]]},"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>To investigate the interplay between anxiety disorders and SLE using lupus-prone MRL-lpr mice and MRL\/MPJ control mice exposed to predator stress, and to elucidate the potential mechanisms underlying this interaction.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>We conducted an experiment where 16 MRL-lpr mice and 16 MRL\/MPJ control mice were randomly assigned to four groups and exposed to predator stress (cat exposure) or served as unexposed controls for 2\u2009months. Anxiety levels were evaluated using the elevated plus maze (EPM) and open field test (OFT). Physiological responses were assessed through measurements of body weight, interleukin-6 (IL-6) levels, anti-double-stranded DNA (dsDNA) antibody titres and urine protein content. Additionally, the splenic index and the proportions of regulatory T (Treg) and T helper 17 (Th17) cells were analysed to further understand the immune responses.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Both mouse strains exhibited increased anxiety levels as assessed by the EPM and OFT. However, MRL-lpr mice demonstrated a heightened sensitivity to predator stress-induced anxiety compared with MRL\/MPJ mice. Biochemical analyses revealed that while MRL\/MPJ mice showed a typical inflammatory response to predator stress, characterised by elevated IL-6 levels, this did not exacerbate immune dysregulation or renal damage. In contrast, MRL-lpr mice exhibited markedly increased IL-6 expression, elevated anti-dsDNA antibody levels, higher urine protein content, decreased Treg proportions and increased Th17 proportions in the spleen, suggesting an accelerated progression of lupus disease.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Our findings emphasise that lupus-prone MRL-lpr mice display a greater vulnerability to the detrimental consequences of predator stress compared with MRL\/MPJ control mice.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1136\/lupus-2025-001528","type":"journal-article","created":{"date-parts":[[2025,6,19]],"date-time":"2025-06-19T23:10:19Z","timestamp":1750374619000},"page":"e001528","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":6,"title":["Effects of environmentally-induced anxiety on autoimmunity in the MRL\/lpr mouse"],"prefix":"10.1136","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7998-1216","authenticated-orcid":false,"given":"Fan","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Pathophysiology, Guizhou Medical University, Guiyang, China"},{"name":"Guizhou Provincial Key Laboratory of Pathogenesis and Drug Research on Common Chronic Diseases, Guizhou Medical University, Guiyang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2850-9009","authenticated-orcid":false,"given":"Qiong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Key Laboratory of Pathogenesis and Drug Research on Common Chronic Diseases, Guizhou Medical University, Guiyang, China"},{"name":"School of Nursing, Guizhou Medical University, Guiyang, 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Laboratory of Pathogenesis and Drug Research on Common Chronic Diseases, Guizhou Medical University, Guiyang, China"},{"name":"School of Nursing, Guizhou Medical University, Guiyang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-1986-563X","authenticated-orcid":false,"given":"Quan-min","family":"Zhou","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Key Laboratory of Pathogenesis and Drug Research on Common Chronic Diseases, Guizhou Medical University, Guiyang, China"},{"name":"School of Nursing, Guizhou Medical University, Guiyang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7880-2260","authenticated-orcid":false,"given":"Xiao-ying","family":"Li","sequence":"additional","affiliation":[{"name":"Guizhou Provincial Key Laboratory of Pathogenesis and Drug Research on Common Chronic Diseases, Guizhou Medical University, Guiyang, China"},{"name":"Department of 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