{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T04:01:32Z","timestamp":1769572892664,"version":"3.49.0"},"reference-count":25,"publisher":"BMJ","issue":"1","license":[{"start":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T00:00:00Z","timestamp":1769472000000},"content-version":"unspecified","delay-in-days":26,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81960306"],"award-info":[{"award-number":["81960306"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82060301"],"award-info":[{"award-number":["82060301"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Key Programs of Natural Science Foundation of Ningxia","award":["2024AAC02047"],"award-info":[{"award-number":["2024AAC02047"]}]}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Lupus Sci Med"],"accepted":{"date-parts":[[2025,12,20]]},"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      SLE is a multifaceted chronic inflammatory disorder characterised by a dysregulated immune response that involves various organ systems, with significant implications stemming from the type I interferon (IFN) signalling pathway in its pathogenesis. This study aimed to elucidate the contributory role of the IFN-induced protein 44-like (\n                      <jats:italic>IFI44L<\/jats:italic>\n                      ) gene in SLE progression and to investigate its transcriptional regulatory mechanisms.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We quantified\n                      <jats:italic>IFI44L<\/jats:italic>\n                      expression in peripheral blood mononuclear cells of patients with SLE through quantitative PCR (qPCR), and established an in vitro THP-1 cell model overexpressing\n                      <jats:italic>IFI44L<\/jats:italic>\n                      to assess its impact under IFN-\u03b1 exposure using Cell Counting Kit-8 assays and flow cytometry. Additionally, we generated\n                      <jats:italic>Ifi44l<\/jats:italic>\n                      knockout mice and Pristane-induced lupus mice to evaluate the influence of\n                      <jats:italic>IFI44L<\/jats:italic>\n                      on immune phenotypes and organ functionality.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Our findings demonstrated that\n                      <jats:italic>IFI44L<\/jats:italic>\n                      is significantly expressed in CD3\n                      <jats:sup>+<\/jats:sup>\n                      and CD14\n                      <jats:sup>+<\/jats:sup>\n                      lymphocytes in patients with SLE, and under heightened IFN conditions, it plays a role in promoting cell proliferation while inhibiting apoptosis. Importantly,\n                      <jats:italic>Ifi44l<\/jats:italic>\n                      knockout mice exhibited ameliorated clinicopathological features of lupus, showing reduced haematological and renal damage. Furthermore, we identified c-Jun as a transcriptional factor that directly targets the\n                      <jats:italic>IFI44L<\/jats:italic>\n                      promoter, specifically activated by IFN-\u03b1 in CD14\n                      <jats:sup>+<\/jats:sup>\n                      lymphocytes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Our research indicates that IFN-\u03b1 enhances\n                      <jats:italic>IFI44L<\/jats:italic>\n                      expression via c-Jun, underscoring its critical role in the pathogenesis of SLE and suggesting potential pathways for therapeutic intervention.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/lupus-2025-001769","type":"journal-article","created":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T15:35:18Z","timestamp":1769528118000},"page":"e001769","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["Regulatory dynamics of\n                    <i>IFI44L<\/i>\n                    in systemic lupus erythematosus: the interplay of type I interferon and c-Jun"],"prefix":"10.1136","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-3221-2847","authenticated-orcid":false,"given":"Yuan","family":"Wang","sequence":"first","affiliation":[{"name":"School of Basic Medicine, Ningxia Medical University, Yinchuan, Ningxia, China"},{"name":"Key Laboratory of Fertility Preservation and Maintenance of the Ministry of Education, Ningxia Medical University, Yinchuan, Ningxia, China"},{"name":"General Hospital of Ningxia Medical University, Yinchuan, Ningxia, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengyi","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Basic Medicine, Ningxia Medical University, Yinchuan, Ningxia, China"},{"name":"Key Laboratory of Fertility Preservation and Maintenance of the Ministry of Education, Ningxia Medical University, Yinchuan, Ningxia, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Ma","sequence":"additional","affiliation":[{"name":"School of Basic Medicine, Ningxia Medical University, Yinchuan, Ningxia, China"},{"name":"Key Laboratory of Fertility Preservation and Maintenance of the Ministry of Education, Ningxia Medical University, Yinchuan, Ningxia, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuhui","family":"Ma","sequence":"additional","affiliation":[{"name":"School of Basic Medicine, Ningxia Medical University, Yinchuan, Ningxia, China"},{"name":"Key Laboratory of Fertility Preservation and Maintenance of the Ministry of Education, Ningxia Medical University, Yinchuan, Ningxia, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenghao","family":"Huo","sequence":"additional","affiliation":[{"name":"School of Basic Medicine, Ningxia Medical University, Yinchuan, Ningxia, China"},{"name":"Key Laboratory of Fertility Preservation and Maintenance of the Ministry of Education, Ningxia Medical University, Yinchuan, Ningxia, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhanbing","family":"Ma","sequence":"additional","affiliation":[{"name":"School of Basic Medicine, Ningxia Medical University, Yinchuan, Ningxia, China"},{"name":"Key Laboratory of Fertility Preservation and Maintenance of the Ministry of Education, Ningxia Medical University, Yinchuan, Ningxia, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Dang","sequence":"additional","affiliation":[{"name":"School of Basic Medicine, Ningxia Medical University, Yinchuan, Ningxia, China"},{"name":"Key Laboratory of Fertility Preservation and Maintenance of the Ministry of Education, Ningxia Medical University, Yinchuan, Ningxia, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2026,1,27]]},"reference":[{"key":"2026012707351083000_13.1.e001769.1","first-page":"715","article-title":"One year in review 2019: systemic lupus erythematosus","volume":"37","author":"Zucchi","year":"2019","journal-title":"Clin Exp Rheumatol"},{"key":"2026012707351083000_13.1.e001769.2","doi-asserted-by":"crossref","DOI":"10.1186\/ar2475","article-title":"Interferon-induced protein IFIT4 is associated with systemic lupus erythematosus and promotes differentiation of monocytes into dendritic cell-like cells","volume":"10","author":"Huang","year":"2008","journal-title":"Arthritis Res 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Study on the relationship of TLR signaling pathway related genes and systemic lupus erythematosus [journal article]. 2011."},{"key":"2026012707351083000_13.1.e001769.22","doi-asserted-by":"crossref","first-page":"366","DOI":"10.1016\/j.phrs.2017.09.014","article-title":"Targeting activator protein 1 signaling pathway by bioactive natural agents: Possible therapeutic strategy for cancer prevention and intervention","volume":"128","author":"Tewari","year":"2018","journal-title":"Pharmacol Res"},{"key":"2026012707351083000_13.1.e001769.23","first-page":"628","article-title":"Expression and function of Cbl-b in T cells from patients with systemic lupus erythematosus, and detection of the 2126 A\/G Cblb gene polymorphism in the Mexican mestizo population","volume":"20","author":"Don\u00edz-Padilla","year":"2011","journal-title":"Lupus (Los Angel)"},{"key":"2026012707351083000_13.1.e001769.24","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M605808200"},{"key":"2026012707351083000_13.1.e001769.25","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1089\/dna.2011.1536","article-title":"Association of c-Jun gene polymorphism with susceptibility to systemic lupus erythematosus in a Chinese population","volume":"31","author":"Ma","year":"2012","journal-title":"DNA Cell Biol"}],"container-title":["Lupus Science &amp; Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/lupus-2025-001769","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T15:35:21Z","timestamp":1769528121000},"score":1,"resource":{"primary":{"URL":"https:\/\/lupus.bmj.com\/lookup\/doi\/10.1136\/lupus-2025-001769"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":25,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2026,1,27]]},"published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1136\/lupus-2025-001769"],"URL":"https:\/\/doi.org\/10.1136\/lupus-2025-001769","relation":{},"ISSN":["2053-8790"],"issn-type":[{"value":"2053-8790","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]}}}