{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T12:36:10Z","timestamp":1788438970244,"version":"build-2803163510"},"reference-count":17,"publisher":"BMJ","issue":"1","license":[{"start":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T00:00:00Z","timestamp":1768176000000},"content-version":"unspecified","delay-in-days":11,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Lupus Sci Med"],"accepted":{"date-parts":[[2025,12,29]]},"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To assess the clinical relevance of concurrent measurement of anti-chromatin antibodies and anti-double-stranded DNA (anti-dsDNA) antibodies using a multiplex immunoassay in systemic lupus erythematosus (SLE), focusing on their association with organ-specific manifestations and disease activity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Adult patients diagnosed with SLE based on the 2012 Systemic Lupus International Collaborating Clinics or 2019 European Alliance of Associations for Rheumatology\/American College of Rheumatology criteria and followed in our university hospital between 2015 and 2019 were retrospectively included if they had at least one positive detection of anti-dsDNA and\/or anti-chromatin result by a multiplex immunoassay, with clinical data available within the 30 days before or after testing. Clinical manifestations, treatments, laboratory parameters and disease activity (SLE Disease Activity Index 2000 (SLEDAI-2K)) were compared using mixed models and correlation coefficients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>98 patients (88%\u2009women; median age 42 (IQR: 35\u201350)) had 677 antibody evaluations. Levels of anti-dsDNA and anti-chromatin antibodies were correlated (r=0.39, 95% CI 0.21 to 0.55). Results were discordant in 59 patients (60%), representing 215 assessments (32%), with anti-chromatin antibodies being detected without anti-dsDNA in 111\/677 samples (16%). Rise in anti-dsDNA antibody titre was associated with cutaneous (OR 2.29, 95% CI 1.43 to 3.68) and musculoskeletal involvement (OR 1.29, 95% CI 1.02 to 1.64), while anti-chromatin antibody increase was linked to neuropsychiatric manifestations (OR 1.54, 95% CI 1.03 to 2.29). The increase of either antibody titre was independently associated with proteinuria &gt;0.5\u2009g\/24\u2009hours (anti-dsDNA antibody: OR 1.17, 95% CI 1.11 to 2.74; anti-chromatin antibody: OR 1.71, 95% CI 1.02 to 2.87). Correlation with SLEDAI-2K was moderate for anti-dsDNA (r=0.55; 95% CI 0.39 to 0.67), and weaker for anti-chromatin (r=0.32; 95% CI 0.16 to 0.51]).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Concomitant measurement of anti-dsDNA and anti-chromatin antibodies using a multiplex immunoassay system brings complementary information for diagnosis and monitoring of SLE. Both antibodies are associated with disease activity and renal involvement, while anti-chromatin antibodies are associated with neuropsychiatric flares. Anti-chromatin antibodies provide additional diagnostic value, particularly in the absence of anti-dsDNA positivity that was observed in 16% of our cohort.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/lupus-2025-001822","type":"journal-article","created":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T16:40:10Z","timestamp":1768236010000},"page":"e001822","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":4,"title":["Clinical value of simultaneous detection of anti-dsDNA and anti-chromatin antibodies using a multiplex immunoassay in systemic lupus erythematosus: a 5-year retrospective study"],"prefix":"10.1136","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2966-6346","authenticated-orcid":false,"given":"Thibault","family":"Sixt","sequence":"first","affiliation":[{"name":"Service de M\u00e9decine Interne et Immunologie Clinique, Centre de R\u00e9f\u00e9rence des Maladies Auto-Immunes Rares de l\u2019Adulte, CHU Dijon-Bourgogne, Dijon, France"},{"name":"Universit\u00e9 Bourgogne Europe, Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Lakomy","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Bourgogne Europe, Dijon, France"},{"name":"Laboratoire d'Immunologie, CHU Dijon-Bourgogne, Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrien","family":"Guilloteau","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Bourgogne Europe, Dijon, France"},{"name":"UMR1231, Equipe Epi2THM, INSERM, Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxime","family":"Samson","sequence":"additional","affiliation":[{"name":"Service de M\u00e9decine Interne et Immunologie Clinique, Centre de R\u00e9f\u00e9rence des Maladies Auto-Immunes Rares de l\u2019Adulte, CHU Dijon-Bourgogne, Dijon, France"},{"name":"Universit\u00e9 Bourgogne Europe, Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"Bonnotte","sequence":"additional","affiliation":[{"name":"Service de M\u00e9decine Interne et Immunologie Clinique, Centre de R\u00e9f\u00e9rence des Maladies Auto-Immunes Rares de l\u2019Adulte, CHU Dijon-Bourgogne, Dijon, France"},{"name":"Universit\u00e9 Bourgogne Europe, Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvain","family":"Audia","sequence":"additional","affiliation":[{"name":"Service de M\u00e9decine Interne et Immunologie Clinique, Centre de R\u00e9f\u00e9rence des Maladies Auto-Immunes Rares de l\u2019Adulte, CHU Dijon-Bourgogne, Dijon, France"},{"name":"Universit\u00e9 Bourgogne Europe, Dijon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2026,1,12]]},"reference":[{"key":"2026011208400484000_13.1.e001822.1","doi-asserted-by":"crossref","first-page":"1400","DOI":"10.1002\/art.40930","article-title":"2019 European League Against Rheumatism\/American College of Rheumatology Classification Criteria for Systemic Lupus Erythematosus","volume":"71","author":"Aringer","year":"2019","journal-title":"Arthritis Rheumatol"},{"key":"2026011208400484000_13.1.e001822.2","doi-asserted-by":"publisher","DOI":"10.1002\/art.34473"},{"key":"2026011208400484000_13.1.e001822.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjmed.2003.08.034"},{"key":"2026011208400484000_13.1.e001822.4","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keh024"},{"key":"2026011208400484000_13.1.e001822.5","first-page":"734","article-title":"Anti-dsDNA antibody assay: high specificity and sensitivity with a filtration radioassay in 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