{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T16:22:04Z","timestamp":1759335724723},"reference-count":28,"publisher":"BMJ","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Occup Environ Med"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:p><jats:bold>Aims:<\/jats:bold>To evaluate potential exposure to<jats:italic>Bacillis anthracis<\/jats:italic>(<jats:italic>Ba<\/jats:italic>) spores in sampling\/decontamination workers in the aftermath of an anthrax terror attack.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold>Fifty six serum samples were obtained from workers involved in environmental sampling for<jats:italic>Ba<\/jats:italic>spores at the American Media, Inc. (AMI) building in Boca Raton, FL after the anthrax attack there in October 2001. Nineteen sera were drawn from individuals both pre-entry and several weeks after entrance into the building. Nine sera each were drawn from unique individuals at the pre-entry and follow up blood draws. Thirteen donor control sera were also evaluated. Individuals were surveyed for<jats:italic>Ba<\/jats:italic>exposure by measurement of serum<jats:italic>Ba<\/jats:italic>anti-protective antigen (PA) specific IgG antibodies using a newly developed fluorescent covalent microsphere immunoassay (FCMIA).<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold>Four sera gave positive anti-PA IgG results (defined as anti-PA IgG concentrations \u2a7e the mean \u03bcg\/ml anti-PA IgG from donor control sera (n\u200a=\u200a13 plus 2 SD which were also inhibited \u2a7e 85% when the serum was pre-adsorbed with PA). The positive sera were the pre-entry and follow up samples of two workers who had received their last dose of anthrax vaccine in 2000.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold>It appears that the sampling\/decontamination workers of the present study either had insufficient exposure to<jats:italic>Ba<\/jats:italic>spores to cause the production of anti-PA IgG antibodies or they were exposed to anthrax spores without producing antibody. The FCMIA appears to be a fast, sensitive, accurate, and precise method for the measurement of anti-PA IgG antibodies.<\/jats:p>","DOI":"10.1136\/oem.2003.008565","type":"journal-article","created":{"date-parts":[[2004,7,16]],"date-time":"2004-07-16T17:15:01Z","timestamp":1089998101000},"page":"703-708","source":"Crossref","is-referenced-by-count":17,"title":["Determination of serum IgG antibodies to<i>Bacillus anthracis<\/i>protective antigen in environmental sampling workers using a fluorescent covalent microsphere immunoassay"],"prefix":"10.1136","volume":"61","author":[{"given":"R E","family":"Biagini","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D L","family":"Sammons","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J P","family":"Smith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E H","family":"Page","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J E","family":"Snawder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C A F","family":"Striley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B A","family":"MacKenzie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2004,7,16]]},"reference":[{"key":"2024052222282369000_61.8.703.1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.287.17.2236"},{"key":"2024052222282369000_61.8.703.2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0810.020354"},{"key":"2024052222282369000_61.8.703.3","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa012948"},{"key":"2024052222282369000_61.8.703.4","doi-asserted-by":"publisher","DOI":"10.1001\/jama.288.22.2853"},{"key":"2024052222282369000_61.8.703.5","first-page":"786","volume":"51","year":"2002","journal-title":"Morb Mortal Weekly Reports"},{"key":"2024052222282369000_61.8.703.6","first-page":"909","volume":"50","year":"2001","journal-title":"Morb Mortal Weekly Reports"},{"key":"2024052222282369000_61.8.703.7","doi-asserted-by":"publisher","DOI":"10.3201\/eid0810.020380"},{"key":"2024052222282369000_61.8.703.8","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1080\/1047322X.1990.10389587","volume":"5","year":"1990","journal-title":"Appl Occup Environ Hyg"},{"key":"2024052222282369000_61.8.703.9","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.5.2888-2893.2001"},{"key":"2024052222282369000_61.8.703.10","unstructured":"Freilich D , Doolan D, Brice G. 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