{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T23:34:13Z","timestamp":1789774453874,"version":"4.0.1"},"reference-count":29,"publisher":"BMJ","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Occup Environ Med"],"published-print":{"date-parts":[[1997,9]]},"abstract":"<jats:p>OBJECTIVE: To investigate the risk of enzyme sensitisation and clinical allergy in workers exposed to enzymes at Novo Nordisk A\/S. METHODS: The study was a retrospective follow up study based on medical history and test data originally collected at routine screenings for enzyme allergy by the Occupational Health Service (OHS) of Novo Nordisk A\/S during the period 1970-92. Workers were exposed to proteases, lipases, cellulases, and carboxyhydrases. Medical records of 3815 subjects were registered in the OHS database. According to criteria including possible enzyme exposure, allergy tests at the time of engagement, and participation in the allergy screening programme 1064 were selected for the present study. Outcomes were allergy symptoms, specific IgE test (radioallergosorbent test (RAST)) to enzymes, skin test reactions to common allergens and enzymes, forced expiratory volume in one second (FEV1), and forced vital capacity (FVC). Potential risk factors were smoking habits, workplace, type of job, age, and sex. RESULTS: Sensitisation occurred to all types of enzymes handled in the plant, most often in production areas and laboratories; 8.8% developed clinical enzyme allergy during the first three years of employment. The risk declined during the period. The frequency of enzyme sensitisation, expressed as RAST values &gt; 0.5 SU, was 36%, and the frequency of significant RAST values &gt; or = 2 SU was 8%. Ranking diagnoses of enzyme allergy by severity, the frequency of asthma was 5.3%, rhinitis 3.0%, and urticaria 0.6%. Half of the cases occurred within the first 15 months of exposure. Smoking was an independent risk factor for clinical enzyme allergy (odds ratio (OR) = 2.3 (95% exact confidence interval (95% CI) 1.4 to 3.9), measurable RAST &gt; or = 0.5 SU (OR = 1.5 (95% CI 1.1 to 2.1)), and RAST &gt; or = 2 SU (OR = 4.5 (95% CI 2.2 to 8.4)). Atopic predisposition at the time of engagement was not a significant risk factor for enzyme allergy. This could be due to various selection mechanisms.<\/jats:p>","DOI":"10.1136\/oem.54.9.671","type":"journal-article","created":{"date-parts":[[2008,12,17]],"date-time":"2008-12-17T00:14:48Z","timestamp":1229472888000},"page":"671-675","source":"Crossref","is-referenced-by-count":49,"title":["Allergy risk in an enzyme producing plant: a retrospective follow up study."],"prefix":"10.1136","volume":"54","author":[{"given":"C R","family":"Johnsen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T B","family":"Sorensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Ingemann Larsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Bertelsen Secher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Andreasen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G S","family":"Kofoed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Fredslund Nielsen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Gyntelberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[1997,9,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1016\/0091-6749(72)90085-1","article-title":"Enzyme allergy in populations exposed to long-term, low-level concentrations of household laundry products","volume":"49","author":"Bernstein, I.L.","year":"1972","unstructured":"Bernstein IL. Enzyme allergy in populations exposed to long-term, low-level concentrations of household laundry products. JAllergy Clin Immunol 1972;49:219-37.","journal-title":"JAllergy Clin Immunol"},{"key":"ref_2","first-page":"43","article-title":"Lung function, atopy, specific hypersensitivity, and smoking of workers in the enzyme detergent industry over 11 years","volume":"42","author":"Flood, D.F.S.; Blofeld, R.E.; Bruce, C.F.; Hewitt, J.I.; Juniper, C.P.; Roberts, D.M.","year":"1985","unstructured":"Flood DFS, Blofeld RE, Bruce CF, Hewitt JI, Juniper CP, Roberts DM. Lung function, atopy, specific hypersensitivity, and smoking of workers in the enzyme detergent industry over 11 years. 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Clin AUergy 1985;15:101-15.","journal-title":"Clin AUergy"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1002\/ajim.4700210414","article-title":"Allergic asthma to Bacillus subtilis enzyme: a model for the effects of inhalable proteins","volume":"21","author":"Pepys, J.","year":"1992","unstructured":"Pepys J. Allergic asthma to Bacillus subtilis enzyme: a model for the effects of inhalable proteins. Am Ind Med 1992;21: 587-93.","journal-title":"Am Ind Med"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1111\/j.1398-9995.1973.tb01445.x","article-title":"Medical experience in enzyme production","volume":"28","year":"1973","unstructured":"Witmeur 0, Wolf-Jurgensen P, H0egh-Thomsen J, Rasmussen OG, Wide L, Zachariae H. Medical experience in enzyme production. Acta Allergol 1973;28:250-9.","journal-title":"Acta Allergol"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1111\/j.1398-9995.1981.tb01864.x","article-title":"Detergent enzymes and occupational safety. Observations on sensitization during Esperase production","volume":"36","year":"1981","unstructured":"Zachariae H, Hoegh-Thomsen J, Witmeur 0, Wide L. Detergent enzymes and occupational safety. Observations on sensitization during Esperase production. Allergy 1981; 36:513-6.","journal-title":"Allergy"},{"key":"ref_8","first-page":"271","article-title":"Laboratory methods in clinical allergy-skin tests for immediate, type","volume-title":"I, allergic reactions. Proc R Soc Med","volume":"65","author":"Pepys, J.","year":"1972","unstructured":"Pepys J. Laboratory methods in clinical allergy-skin tests for immediate, type I, allergic reactions. Proc R Soc Med 1972;65:271-2."},{"key":"ref_9","first-page":"412","article-title":"Skin testing","volume":"14","author":"Pepys, J.","year":"1975","unstructured":"Pepys J. Skin testing. BrJHosp Med 1975;14:412-7.","journal-title":"BrJHosp Med"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1016\/S0140-6736(69)90165-2","article-title":"Reaginic antibody (IgE), skin, and provocation tests to dermatophagoides culinx and house dust in respiratory allergy","volume":"ii:","author":"Stenius, B.; Wide, L.","year":"1969","unstructured":"Stenius B, Wide L. Reaginic antibody (IgE), skin, and provocation tests to dermatophagoides culinx and house dust in respiratory allergy. Lancet 1969;ii:455-8.","journal-title":"Lancet"},{"key":"ref_11","first-page":"1105","article-title":"Diagnosis of allergy by an in-vitro test for allergen antibodies","volume":"ii:","author":"Wide, L.; Bennich, H.; Johansson, S.G.O.","year":"1967","unstructured":"Wide L, Bennich H, Johansson SGO. Diagnosis of allergy by an in-vitro test for allergen antibodies. Lancet 1967;ii: 1105-7.","journal-title":"Lancet"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1111\/j.1365-2222.1974.tb01385.x","article-title":"IgE-antibodies and skin test reactions to a detergent-enzyme in Swedish consumers","volume":"4","author":"Zetterstrom 0, Wide L","year":"1974","unstructured":"Zetterstrom 0, Wide L. IgE-antibodies and skin test reactions to a detergent-enzyme in Swedish consumers. Clin Allergy 1974;4:273-80.","journal-title":"Clin Allergy"},{"key":"ref_13","first-page":"11","article-title":"Skin tests used in type I allergy testing","volume":"44","author":"Dreborg, S.","year":"1995","unstructured":"Dreborg S, ed. Skin tests used in type I allergy testing. Allergy 1995;44:11-59.","journal-title":"Allergy"},{"key":"ref_14","first-page":"4","article-title":"Altergi i en dansk voksenbefolkning - belyst ved priktestning med inhalationsallergener. (Allergy in a Danish adult population - illustrated by prick test with inhalable allergens","author":"Nielsen, N.H.","year":"1993","unstructured":"Nielsen NH. Altergi i en dansk voksenbefolkning - belyst ved priktestning med inhalationsallergener. (Allergy in a Danish adult population - illustrated by prick test with inhalable allergens [thesis]. Copenhagen: University of Copenhagen, 1993:4-66. (Summary in English.)"},{"key":"ref_15","first-page":"3211","article-title":"Forekomst af medicinsk-allergiske sygdomme i speciallsgeregie (Cutaneous allergy in a Danish multicenter study)","volume-title":"Ugeskr Laeger","volume":"143","year":"1981","unstructured":"Osterballe 0, Dirksen A, Weeke B, Weeke ER. Forekomst af medicinsk-allergiske sygdomme i speciallsgeregie (Cutaneous allergy in a Danish multicenter study). Ugeskr Laeger 1981;143:3211-8. 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Lancet 1969;1:1177-81.","journal-title":"Lancet"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1111\/j.1398-9995.1972.tb01644.x","article-title":"Medical problem in the detergent industry caused by proteolytic enzymes from bacillus subtilis","volume":"27","author":"Gothe, C.; Nilzen, A.; Hohmgren, A.; Szamosi, A.; Werner, M.; Wide, L.","year":"1972","unstructured":"Gothe C, Nilzen A, Hohmgren A, Szamosi A, Werner M, Wide L. Medical problem in the detergent industry caused by proteolytic enzymes from bacillus subtilis. Acta Allergol 1972;27:63-86.","journal-title":"Acta Allergol"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1136\/thx.31.6.621","article-title":"Biological effects of proteolytic enzyme detergents","volume":"31","author":"Gilson, J.C.; Juniper, G.P.; Martin, R.B.; Weill, H.","year":"1976","unstructured":"Gilson JC, Juniper GP, Martin RB, Weill H. Biological effects of proteolytic enzyme detergents. Thorax 1976;31: 621-34.","journal-title":"Thorax"},{"key":"ref_20","first-page":"1","article-title":"Methods of measuring biologically active enzyme dust in environmental air of detergent factories","volume":"21","author":"Bruce, C.F.; Dunn, E.; Brotherton, R.; Davies, D.R.; Hall, F.; Potts, S.C.M.","year":"1976","unstructured":"Bruce CF, Dunn E, Brotherton R, Davies DR, Hall F, Potts SCM. Methods of measuring biologically active enzyme dust in environmental air of detergent factories. Ann Occup Hyg 1976;21:1-20.","journal-title":"Ann Occup Hyg"},{"key":"ref_21","first-page":"107","article-title":"Occupational asthma","author":"Montanaro, A.","year":"1992","unstructured":"Montanaro A. Occupational asthma. Philadelphia: Hanley and Belfus, 1992:107-130."},{"key":"ref_22","first-page":"636","article-title":"Respiratory symptoms, lung function, and sensitisation to flour in a British bakery","volume":"46","author":"Musk, A.W.; Venables, K.M.; Crook, B.","year":"1989","unstructured":"Musk AW, Venables KM, Crook B, et al. Respiratory symptoms, lung function, and sensitisation to flour in a British bakery. BrJInd Med 1989;46:636-42.","journal-title":"BrJInd Med"},{"key":"ref_23","article-title":"Forebyggelse afalergi hos bagere","author":"Wilhardt, P.; Mikkelsen, S.; Nuchel Petersen, L.; Wittrock, J.","year":"1993","unstructured":"Wilhardt P, Mikkelsen S, Nuchel Petersen L, Wittrock J. Forebyggelse afalergi hos bagere. Copenhagen: Arbejdsmilj0- fondet, 1993."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1111\/j.1398-9995.1982.tb01917.x","article-title":"Coffee worker's allergy","volume":"37","year":"1982","unstructured":"Osterman K, Zetterstrom 0, Johansson SGO. Coffee worker's allergy. Allergy 1982;37:313-22.","journal-title":"Allergy"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1007\/BF02914472","article-title":"Occupational reactions in the seafood industry","volume":"11","author":"Malo, J.; Cartier, A.","year":"1993","unstructured":"Malo J, Cartier A. Occupational reactions in the seafood industry. Clin Rev Allergy 1993; 11:223-39.","journal-title":"Clin Rev Allergy"},{"key":"ref_26","first-page":"930","article-title":"Immunological and clinical findings in workers and consumers exposed to the enzymes","volume-title":"of Bacillus subtilis. Proc R Soc Med","volume":"66","author":"Pepys, J.","year":"1973","unstructured":"Pepys J. Immunological and clinical findings in workers and consumers exposed to the enzymes of Bacillus subtilis. Proc R Soc Med 1973;66:930-3."},{"key":"ref_27","first-page":"1","article-title":"American Conference of Governmental Industrial Hygienists. Threshold limit valuesfor chemical substances and physical agents and biological exposure indices","year":"1993","unstructured":"American Conference of Governmental Industrial Hygienists. Threshold limit valuesfor chemical substances and physical agents and biological exposure indices. Cincinnati: ACGIH, 1993:1-124."},{"key":"ref_28","first-page":"236","article-title":"American Conference of Governmental Industrial Hygienists. Threshold limit values of airborne-contaminants and intended changes. Adopted by ACGIH for 1970","year":"1970","unstructured":"American Conference of Governmental Industrial Hygienists. Threshold limit values of airborne-contaminants and intended changes. Adopted by ACGIH for 1970. Cincinnati: ACGIH, 1970:236-8."},{"issue":"118","key":"ref_29","first-page":"5","article-title":"Enzyme-containing laundering compounds and consumer health","volume":"204","year":"1971","unstructured":"Enzyme-containing laundering compounds and consumer health. Washington, DC: Division of Medical Sciences, National Academy of Sciences - National Research Council, 1971:5-31. 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