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However, diagnostic accuracy of selective myocardial perfusion imaging after coronary CTA is currently unclear. The Danish study of Non-Invasive testing in Coronary Artery Disease 3 trial is designed to evaluate head to head the diagnostic accuracy of myocardial perfusion imaging with positron emission tomography (PET) using the tracers<jats:sup>82<\/jats:sup>Rubidium (<jats:sup>82<\/jats:sup>Rb-PET) compared with oxygen-15 labelled water PET (<jats:sup>15<\/jats:sup>O-water-PET) in patients with symptoms of obstructive CAD and a coronary CT scan with suspected obstructive CAD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and analysis<\/jats:title><jats:p>This prospective, multicentre, cross-sectional study will include approximately 1000 symptomatic patients without previous CAD. Patients are included after referral to coronary CTA. All patients undergo a structured interview and blood is sampled for genetic and proteomic analysis and a coronary CTA. Patients with possible obstructive CAD at coronary CTA are examined with both<jats:sup>82<\/jats:sup>Rb-PET,<jats:sup>15<\/jats:sup>O-water-PET and invasive coronary angiography with three-vessel fractional flow reserve and thermodilution measurements of coronary flow reserve. After enrolment, patients are followed with Seattle Angina Questionnaires and follow-up PET scans in patients with an initially abnormal PET scan and for cardiovascular events in 10 years.<\/jats:p><\/jats:sec><jats:sec><jats:title>Ethics and dissemination<\/jats:title><jats:p>Ethical approval was obtained from Danish regional committee on health research ethics. Written informed consent will be provided by all study participants. 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