{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T05:15:58Z","timestamp":1783055758584,"version":"3.54.6"},"reference-count":32,"publisher":"BMJ","issue":"2","license":[{"start":{"date-parts":[[2025,8,8]],"date-time":"2025-08-08T00:00:00Z","timestamp":1754611200000},"content-version":"unspecified","delay-in-days":38,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/3.0\/igo\/"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Open Heart"],"accepted":{"date-parts":[[2025,6,10]]},"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Bartonella<\/jats:italic>\n                      species are an important emerging cause of blood culture-negative endocarditis (BCNE). The diagnosis requires serology by indirect immunofluorescence assay (IFA) and PCR testing on blood and\/or tissue. Access to the guideline-referenced in-house IFA is limited in Africa and a commercially available IFA is used to identify patients with\n                      <jats:italic>Bartonella<\/jats:italic>\n                      spp. infection in our region. Prior study in South Africa has highlighted a high seroprevalence of\n                      <jats:italic>Bartonella<\/jats:italic>\n                      spp. in the general population. It is unclear how to incorporate these factors into the diagnostic thinking when interpreting a positive IFA result in a patient with BCNE. We explore these important knowledge gaps in a cohort of 31 patients with\n                      <jats:italic>Bartonella<\/jats:italic>\n                      endocarditis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>\n                      Data from the Tygerberg Endocarditis Cohort Study were evaluated between October 2019 and May 2023. Continuous variables were reported as mean with SD if normally distributed, alternatively as median with IQR. Categorical variables were reported as counts and percentages. A Kaplan-Meier curve will be used to depict the mortality rate of operated versus unoperated patients. The mean age (\u00b1SD) was 38\u00b19 years, 70.9% were male, 25.8% were either homeless or lived in informal housing, 70.9% were unemployed, 54.8% had an underlying alcohol-use disorder and 25.8% were HIV positive. Blood serology was positive (IgG titre \u22651:256) in 96.7% of patients with available sera. Valvular tissue was available for PCR testing in 18 cases. Of these,\n                      <jats:italic>Bartonella quintana<\/jats:italic>\n                      was identified in 16 cases and\n                      <jats:italic>Bartonella henselae<\/jats:italic>\n                      in one case. None of the cases with both serology and valve PCR data had negative serology. No cases of blood culture-positive endocarditis (BCPE) had a positive PCR for\n                      <jats:italic>Bartonella<\/jats:italic>\n                      spp. The most common isolated valve lesion on echocardiography was severe aortic regurgitation (43.3%). The 1-month and 6-month mortality in the operated cohort was 0% and 4.5%, respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Bartonella quintana<\/jats:italic>\n                      is the most common cause of BCNE accounting for 49.2% of cases at our centre. None of the PCR-proven cases of\n                      <jats:italic>Bartonella<\/jats:italic>\n                      endocarditis had negative serology, which suggests that the test has a high negative predictive value. The current guideline diagnostic titre of \u22651:800 is assay dependent and is not generalisable to alternative, commercially available, assays. Crucially, none of the BCPE cases with available PCR on valve tissue had a positive PCR for\n                      <jats:italic>Bartonella<\/jats:italic>\n                      spp, suggesting a positive valve PCR is definitive evidence of true infection in an endemic region. The specific clinical, echocardiographic and mortality data were consistent with the reported literature and characterised a subacute, but ultimately destructive endocarditis with a high embolic risk and underscores the need for early surgical intervention.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/openhrt-2025-003463","type":"journal-article","created":{"date-parts":[[2025,8,8]],"date-time":"2025-08-08T08:05:25Z","timestamp":1754640325000},"page":"e003463","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":1,"title":["<i>Bartonella<\/i>\n                    endocarditis: a complex diagnosis of blood culture-negative endocarditis in an endemic region of Africa"],"prefix":"10.1136","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7410-1339","authenticated-orcid":false,"given":"Luke D","family":"Hunter","sequence":"first","affiliation":[{"name":"Division of Cardiology, Department of Medicine, Faculty of Medicine and Health Sciences, Stellenbosch University and Tygerberg Hospital, Cape Town, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon","family":"Poerstamper","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Medicine, Faculty of Medicine and Health Sciences, Stellenbosch University and Tygerberg Hospital, Cape Town, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip G","family":"Herbst","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Medicine, Faculty of Medicine and Health Sciences, Stellenbosch University and Tygerberg Hospital, Cape Town, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anton F","family":"Doubell","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Medicine, Faculty of Medicine and Health Sciences, Stellenbosch University and Tygerberg Hospital, Cape Town, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colette","family":"Pienaar","sequence":"additional","affiliation":[{"name":"Division of Medical Microbiology, Department of Pathology, Faculty of Medicine and Health Sciences, Stellenbosch University and National Health Laboratory Service, Tygerberg Hospital, Cape Town, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jantjie J","family":"Taljaard","sequence":"additional","affiliation":[{"name":"Division of Cardiothoracic Surgery, Department of Surgery, Faculty of Medicine and Health Sciences, Stellenbosch University and Tygerberg Hospital, Cape Town, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9715-3449","authenticated-orcid":false,"given":"Hans","family":"Prozesky","sequence":"additional","affiliation":[{"name":"Division of Cardiothoracic Surgery, Department of Surgery, Faculty of Medicine and Health Sciences, Stellenbosch University and Tygerberg Hospital, Cape Town, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques J","family":"Janson","sequence":"additional","affiliation":[{"name":"Division of Infectious Disease, Department of Medicine, Faculty of Medicine and Health Sciences, Stellenbosch University and Tygerberg Hospital, Cape Town, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6549-9402","authenticated-orcid":false,"given":"Alfonso JK","family":"Pecoraro","sequence":"additional","affiliation":[{"name":"Division of Cardiology, Department of Medicine, Faculty of Medicine and Health Sciences, Stellenbosch University and Tygerberg Hospital, Cape Town, South 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