{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,4,27]],"date-time":"2023-04-27T14:41:03Z","timestamp":1682606463975},"reference-count":20,"publisher":"Oxford University Press (OUP)","issue":"818","license":[{"start":{"date-parts":[[1993,12,1]],"date-time":"1993-12-01T00:00:00Z","timestamp":754704000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1993,12,1]]},"abstract":"<jats:title>Summary<\/jats:title>\n               <jats:p>We have used the enzymatic in situ hybridization method to investigate the presence of Epstein-Barr virus (EBV) genome in lymph node tissues from patients with Hodgkin's disease. Also, 11 patients with persistent human immuno-deficiency virus-associated generalized lymphadenopathy as well as seven autopsy cases with no Hodgkin's disease, formed part of these studies.<\/jats:p>\n               <jats:p>EBV DNA-positive reaction was demonstrated in Reed-Sternberg cells and variants in seven of 16 cases and in the small accompanying lymphocyte cell population in 14 of the 16 cases. It was also found in eight of the 11 cases with persistent generalized lymphadenopathy but in none of the lymph nodes from negative selective autopsy cases.<\/jats:p>\n               <jats:p>Our results indicate that the colorimetric in situ hybridization technique is useful in EBV nucleic acid detection and cell-type localization in Hodgkin's disease. Additionally, the detection of EBV genome, not only in the diagnostic cells but in the small lymphocyte cell components, could provide new insights into the potential role of this agent in the pathogenesis of Hodgkin's disease.<\/jats:p>","DOI":"10.1136\/pgmj.69.818.916","type":"journal-article","created":{"date-parts":[[2008,12,29]],"date-time":"2008-12-29T21:25:21Z","timestamp":1230585921000},"page":"916-919","source":"Crossref","is-referenced-by-count":1,"title":["Detection of Epstein-Barr genome in the lymph nodes of Hodgkin's disease"],"prefix":"10.1093","volume":"69","author":[{"given":"C","family":"Bellas","sequence":"first","affiliation":[{"name":"Departments of Pathology and 'Internal Medicine, Hospital Ramon y Cajal , Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F","family":"Mampaso","sequence":"additional","affiliation":[{"name":"Departments of Pathology and 'Internal Medicine, Hospital Ramon y Cajal , Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Fraile","sequence":"additional","affiliation":[{"name":"Departments of Pathology and 'Internal Medicine, Hospital Ramon y Cajal , Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Molina","sequence":"additional","affiliation":[{"name":"Departments of Pathology and 'Internal Medicine, Hospital Ramon y Cajal , Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Bricio","sequence":"additional","affiliation":[{"name":"Departments of Pathology and 'Internal Medicine, Hospital Ramon y Cajal , Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Cuesta","sequence":"additional","affiliation":[{"name":"Departments of Pathology and 'Internal Medicine, Hospital Ramon y Cajal , Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1993,12,1]]},"reference":[{"issue":"1","key":"ref_1","first-page":"702","article-title":"Virus particles in cultured lymphoblasts from Burkitt's lymphoma","volume":"1964","author":"Epstein, M.A.; Achong, B.G.; Barr, Y.M.","unstructured":"Epstein, M.A., Achong, B.G. & Barr, Y.M. Virus particles in cultured lymphoblasts from Burkitt's lymphoma. Lancet 1964, 1: 702-703.","journal-title":"Lancet"},{"issue":"39","key":"ref_2","first-page":"466","article-title":"Epstein-Barr virus, immunodeficiency and B-cell lymphoproliferation","volume":"1985","author":"Hanto, D.W.; Frizzera, G.; Gajl-Peczalska, J.; Simmons, R.L.","unstructured":"Hanto, D.W., Frizzera, G., Gajl-Peczalska, J. & Simmons, R.L. Epstein-Barr virus, immunodeficiency and B-cell lymphoproliferation. Transplantation 1985, 39: 466-472.","journal-title":"Transplantation"},{"key":"ref_3","first-page":"769","article-title":"Relationship between Epstein-Barr virus (EBV) DNA and the EBV-determined nuclear antigen (EBNA) in Burkitt's lymphoma biopsies and other lymphoproliferative malignancies","volume":"13","author":"Lindahl, T.; Klein, G.; Reedman, B.M.; Johnansson, B.; Singh, S.","year":"1974","unstructured":"Lindahl, T., Klein, G., Reedman, B.M., Johnansson, B. & Singh, S. Relationship between Epstein-Barr virus (EBV) DNA and the EBV-determined nuclear antigen (EBNA) in Burkitt's lymphoma biopsies and other lymphoproliferative malignancies. Int J Cancer 1974, 13: 769-772.","journal-title":"Int J Cancer"},{"issue":"162","key":"ref_4","first-page":"603","article-title":"Cancer is a complication of severe immunosuppression","volume":"1986","author":"Penn, I.","unstructured":"Penn, I. Cancer is a complication of severe immunosuppression. Surg Gynecol Obstet 1986, 162: 603-610.","journal-title":"Surg Gynecol Obstet"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"810","DOI":"10.1182\/blood.V74.2.810.810","article-title":"Demonstration of monoclonal EBV genome in Hodgkin's disease and KI-1 positive anaplastic large cell lymphoma by combined Southern blot and in situ hybridization","volume":"74","author":"Anagnostopoulos, I.; Herbst, H.; Niedobitek, G.; Stein, H.","year":"1989","unstructured":"Anagnostopoulos, I., Herbst, H., Niedobitek, G. & Stein, H. Demonstration of monoclonal EBV genome in Hodgkin's disease and KI-1 positive anaplastic large cell lymphoma by combined Southern blot and in situ hybridization. Blood 1989, 74: 810-816.","journal-title":"Blood"},{"key":"ref_6","first-page":"1","article-title":"A survey of Epstein-Barr virus DNA in lymphoid tissue. Frequent detection in Hodgkin's disease","volume":"91","author":"Staal, S.P.; Ambinder, R.; Beschorner, W.E.; Hayward, G.S.; Mann, R.","year":"1984","unstructured":"Staal, S.P., Ambinder, R., Beschorner, W.E., Hayward, G.S. & Mann, R. A survey of Epstein-Barr virus DNA in lymphoid tissue. Frequent detection in Hodgkin's disease. Am J Clin Pathol 1984, 91: 1-5.","journal-title":"Am J Clin Pathol"},{"key":"ref_7","first-page":"86","article-title":"Epstein-Barr viral DNA in tissues of Hodgkin's disease","volume":"129","author":"Weiss, L.M.; Strickler, J.G.; Warnke, R.A.; Purtilo, D.T.; Sklar, J.","year":"1987","unstructured":"Weiss, L.M., Strickler, J.G., Warnke, R.A., Purtilo, D.T. & Sklar, J. Epstein-Barr viral DNA in tissues of Hodgkin's disease. Am J Pathol 1987, 129: 86-91.","journal-title":"Am J Pathol"},{"key":"ref_8","first-page":"13","article-title":"High incidence of Epstein-Barr virus genomes in Hodgkin's disease","volume":"137","author":"Herbst, H.; Niedobitek, G.; Kneba, M.","year":"1990","unstructured":"Herbst, H., Niedobitek, G., Kneba, M. et al. High incidence of Epstein-Barr virus genomes in Hodgkin's disease. Am J Pathol 1990, 137: 13-18.","journal-title":"Am J Pathol"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1056\/NEJM198902233200806","article-title":"Detection of Epstein-Barr viral genomes in Reed-Sternberg cells of Hodgkin's disease","volume":"320","author":"Weiss, L.M.; Movahed, L.A.; Warnke, R.A.; Sklar, J.","year":"1989","unstructured":"Weiss, L.M., Movahed, L.A., Warnke, R.A. & Sklar, J. Detection of Epstein-Barr viral genomes in Reed-Sternberg cells of Hodgkin's disease. N EnglJ Med 1989, 320: 502-506.","journal-title":"N EnglJ Med"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/0042-6822(83)90460-9","article-title":"Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes","volume":"126","author":"Brigati, D.J.; Myerson, D.; Leary, J.J.; Spalholz, B.; Travis, S.Z.; Fong, C.K.Y.","year":"1983","unstructured":"Brigati, D.J., Myerson, D., Leary, J.J., Spalholz, B., Travis, S.Z., Fong, C.K.Y. et al. Detection of viral genomes in cultured cells and paraffin-embedded tissue sections using biotin-labeled hybridization probes. Virology 1983, 126: 32-50.","journal-title":"Virology"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2999","DOI":"10.1073\/pnas.77.5.2999","article-title":"Epstein-Barr virus (B95-8) DNA VII: molecular cloning and detailed mapping","volume":"77","author":"Dambough, T.; Beisel, C.; Hummel, M.","year":"1980","unstructured":"Dambough, T., Beisel, C., Hummel, M. et al. Epstein-Barr virus (B95-8) DNA VII: molecular cloning and detailed mapping. Proc Natl Acad Sci USA 1980, 77: 2999-3003.","journal-title":"Proc Natl Acad Sci USA"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1136\/pgmj.68.803.739","article-title":"In situ demonstration of Epstein-Barr virus in intravenous drug abusers with generalized lymphadenopathy","volume":"68","author":"Mampaso, F.; Bellas, C.; Molina, A.; Quereda, C.; Bricio, T.; Buzon, L.","year":"1992","unstructured":"Mampaso, F., Bellas, C., Molina, A., Quereda, C., Bricio, T. & Buzon, L. 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Hodgkin's disease in patients with previous infectious mononucleosis. Int J Cancer 1979, 23: 593-597.","journal-title":"Int J Cancer"},{"issue":"84","key":"ref_15","first-page":"385","article-title":"Lymphadenopathy consistent with Hodgkin's disease associated with Epstein-Barr virus infection","volume":"1985","author":"Popema, S.; van Imhoff, G.; Toresnma, R.; Smit, J.","unstructured":"Popema, S., van Imhoff, G., Toresnma, R. & Smit, J. Lymphadenopathy consistent with Hodgkin's disease associated with Epstein-Barr virus infection. Am J Clin Pathol 1985, 84: 385-390.","journal-title":"Am J Clin Pathol"},{"key":"ref_16","first-page":"977","article-title":"Persistent active Epstein -Barr virus infection and atypical lymphoproliferation. Report of two cases","volume":"11","author":"Krueger, G.R.F.; Papadakis, T.; Schaefer, H.J.","year":"1987","unstructured":"Krueger, G.R.F., Papadakis, T. & Schaefer, H.J. Persistent active Epstein -Barr virus infection and atypical lymphoproliferation. Report of two cases. Am J Surg Pathol 1987, 11: 977-981.","journal-title":"Am J Surg Pathol"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1002\/ijc.2910400205","article-title":"Clonal rearrangements ofT-cell receptor and immunoglobulin genes and immunophenotypic expression in different subclasses of Hodgkin's disease","volume":"40","author":"Griesser, H.; Feller, A.C.; Mak, T.W.; Lennert, K.","year":"1987","unstructured":"Griesser, H., Feller, A.C., Mak, T.W. & Lennert, K. Clonal rearrangements ofT-cell receptor and immunoglobulin genes and immunophenotypic expression in different subclasses of Hodgkin's disease. 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Persistence of Epstein-Barr virus in Reed-Stemnberg cells throughout the course of Hodgkin's disease. J Pathol 1991, 164: 291-297.","journal-title":"J Pathol"},{"key":"ref_20","first-page":"39","article-title":"EpsteinBarr viral genome in lymph nodes from patients with Hodgkin's disease may not be specific to Reed-Sternberg cells","volume":"139","author":"Mashih, A.; Weisenberger, D.; Duggan, M.","year":"1991","unstructured":"Mashih, A., Weisenberger, D., Duggan, M. et al. EpsteinBarr viral genome in lymph nodes from patients with Hodgkin's disease may not be specific to Reed-Sternberg cells. 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