{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T10:26:06Z","timestamp":1771669566412,"version":"3.50.1"},"reference-count":38,"publisher":"BMJ","issue":"2","license":[{"start":{"date-parts":[[2020,8,26]],"date-time":"2020-08-26T00:00:00Z","timestamp":1598400000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Asociaci\u00f3n Colombiana de Reumatolog\u00eda"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["RMD Open"],"accepted":{"date-parts":[[2020,8,1]]},"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:sec id=\"s1\">\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>To determine the association between endoplasmic reticulum aminopeptidase (ERAP)1 and ERAP2 single-nucleotide polymorphisms (SNPs) and human leukocyte antigens (HLA)-B27+ or HLA-B15+ patients with spondyloarthritis (SpA).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"s2\">\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>104 patients with SpA according to Assessment of Spondyloarthritis International Society criteria were included in the study. HLA typing was performed by PCR. The polymorphisms were determined by real-time PCR on genomic DNA using customised probes for SNPs rs27044, rs17482078, rs10050860 and rs30187 in <jats:italic>ERAP1<\/jats:italic>, and rs2910686, rs2248374 and rs2549782 in <jats:italic>ERAP2<\/jats:italic>.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"s3\">\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>70 of the104 patients with SpA were HLA-B27+ and 34 were HLA-B15+. The distribution of ERAP1 and ERAP2 SNPs between the HLA-B15+ and HLA-B27+ patients with SpA did not reveal differences. Likewise, no differences in the frequencies of ERAP1 SNP haplotypes and alleles HLA-B15 or HLA-B27 were found. Interestingly, however, the frequencies of three particular haplotypes formed by ERAP2 SNPs rs2549782\/rs2248374\/rs2910686 varied between HLA-B15+ and HLA-B27+ patients: the ERAP2 SNPs haplotype TGT was more common in HLA-B15+ patients with SpA (OR 2.943, 95% CI 1.264 to 6.585; P=0.009), whereas the ERAP2 SNP haplotypes TGC and CAT were more associated with HLA-B27+ patients with SpA: (OR 4.483, 95% CI 1.524 to 13.187; p=0.003) and (OR 9.014, 95% CI 1.181 to 68.807; p=0.009), respectively.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec id=\"s4\">\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>An association was found between HLA-B15+ patients with SpA and haplotype TGT of ERAP2 SNPs. On the other hand, HLA-B27+ patients with SpA were associated with ERAP2 haplotypes TGC and CAT. These associations could be related to the clinical presentation of the disease, specifically with a peripheral or axial predominance, respectively.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1136\/rmdopen-2020-001250","type":"journal-article","created":{"date-parts":[[2020,9,11]],"date-time":"2020-09-11T07:30:13Z","timestamp":1599809413000},"page":"e001250","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":7,"title":["Association of ERAP2 polymorphisms in Colombian HLA-B27+ or HLA-B15+ patients with SpA and its relationship with clinical presentation: axial or peripheral predominance"],"prefix":"10.1136","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1074-644X","authenticated-orcid":false,"given":"John","family":"Londono","sequence":"first","affiliation":[{"name":"Department of Rheumatology, Universidad de la Sabana, Chia, Colombia"},{"name":"Department of Rheumatology, Hospital Militar Central, Bogota, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana M","family":"Santos","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Universidad de la Sabana, Chia, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan C","family":"Rueda","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Universidad de la Sabana, Chia, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrique","family":"Calvo-Paramo","sequence":"additional","affiliation":[{"name":"Department of Radiology, Universidad Nacional de Colombia, Bogota, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruben","family":"Burgos-Vargas","sequence":"additional","affiliation":[{"name":"Servicio de Reumatologia, Hospital General de Mexico, Mexico, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gilberto","family":"Vargas-Alarcon","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Instituto Nacional de Cardiologia Ignacio Chavez, Mexico, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy","family":"Martinez-Rodriguez","sequence":"additional","affiliation":[{"name":"Community Health Research Department, Hospital Infantil de Mexico Federico Gomez, Mexico City, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sofia","family":"Arias-Correal","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Universidad de la Sabana, Chia, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guisselle-Nathalia","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Universidad de la Sabana, Chia, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diana","family":"Padilla","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Universidad de la Sabana, Chia, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francy","family":"Cuervo","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Universidad de la Sabana, Chia, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viviana","family":"Reyes-Martinez","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Universidad de la Sabana, Chia, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7010-8338","authenticated-orcid":false,"given":"Santiago","family":"Bernal-Mac\u00edas","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Universidad de la Sabana, Chia, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catalina","family":"Villota-Eraso","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Universidad de la Sabana, Chia, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luz M","family":"Avila-Portillo","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Universidad de la Sabana, Chia, Colombia"},{"name":"Department of Rheumatology, Hospital Militar Central, Bogota, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Consuelo","family":"Romero","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Hospital Militar Central, Bogota, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan F","family":"Medina","sequence":"additional","affiliation":[{"name":"Clinical Training Unit, School of Medicine, Universidad de Navarra, Pamplona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2020,9,10]]},"reference":[{"key":"2025090313003562000_6.2.e001250.1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2009.108217"},{"key":"2025090313003562000_6.2.e001250.2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2009.108233"},{"key":"2025090313003562000_6.2.e001250.3","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2010.133645"},{"key":"2025090313003562000_6.2.e001250.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.berh.2014.10.018"},{"key":"2025090313003562000_6.2.e001250.5","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2010.140574"},{"key":"2025090313003562000_6.2.e001250.6","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2007.17"},{"key":"2025090313003562000_6.2.e001250.7","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1038\/nrrheum.2015.133","article-title":"Genetics of ankylosing spondylitis: insights into pathogenesis","volume":"12","author":"Brown","year":"2016","journal-title":"Nat Rev Rheumatol"},{"key":"2025090313003562000_6.2.e001250.8","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvt066"},{"key":"2025090313003562000_6.2.e001250.9","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2010.85"},{"key":"2025090313003562000_6.2.e001250.10","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2008.104018"},{"key":"2025090313003562000_6.2.e001250.11","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2012.41"},{"key":"2025090313003562000_6.2.e001250.12","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0079596","article-title":"Concerted in vitro trimming of viral HLA-B27-restricted ligands by human ERAP1 and ERAP2 aminopeptidases","volume":"8","author":"Lorente","year":"2013","journal-title":"PLoS One"},{"key":"2025090313003562000_6.2.e001250.13","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2015-207416"},{"key":"2025090313003562000_6.2.e001250.14","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1007\/s10067-016-3516-2","article-title":"Association of human leukocyte A, B, and DR antigens in Colombian patients with diagnosis of spondyloarthritis","volume":"36","author":"Santos","year":"2017","journal-title":"Clin Rheumatol"},{"key":"2025090313003562000_6.2.e001250.15","doi-asserted-by":"crossref","DOI":"10.1136\/bmjopen-2015-009092","article-title":"Analysis of HLA-B15 and HLA-B27 in spondyloarthritis with peripheral and axial clinical patterns","volume":"5","author":"Londono","year":"2015","journal-title":"BMJ Open"},{"key":"2025090313003562000_6.2.e001250.16","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780341003"},{"key":"2025090313003562000_6.2.e001250.17","first-page":"2286","article-title":"A new approach to defining disease status in ankylosing spondylitis: the Bath Ankylosing Spondylitis Disease Activity Index","volume":"21","author":"Garrett","year":"1994","journal-title":"J Rheumatol"},{"key":"2025090313003562000_6.2.e001250.18","first-page":"2281","article-title":"A new approach to defining functional ability in ankylosing spondylitis: the development of the Bath Ankylosing Spondylitis Functional Index","volume":"21","author":"Calin","year":"1994","journal-title":"J Rheumatol"},{"key":"2025090313003562000_6.2.e001250.19","doi-asserted-by":"publisher","DOI":"10.1136\/ard.46.3.197"},{"key":"2025090313003562000_6.2.e001250.20","doi-asserted-by":"publisher","DOI":"10.1111\/j.1755-0998.2010.02847.x"},{"key":"2025090313003562000_6.2.e001250.21","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-8286.2007.01931.x"},{"key":"2025090313003562000_6.2.e001250.22","unstructured":"Bennett PH , Burch TA . The epidemiological diagnosis of ankylosing spondylitis. In: Bennett PH , Wood PHN , eds. Population studies of the rheumatic diseases . Amsterdam: Excerpta Medica, 1968: 453\u20138."},{"key":"2025090313003562000_6.2.e001250.23","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2009.110767"},{"key":"2025090313003562000_6.2.e001250.24","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bth457"},{"key":"2025090313003562000_6.2.e001250.25","doi-asserted-by":"publisher","DOI":"10.1002\/art.24467"},{"key":"2025090313003562000_6.2.e001250.26","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddp371"},{"key":"2025090313003562000_6.2.e001250.27","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2667"},{"key":"2025090313003562000_6.2.e001250.28","doi-asserted-by":"crossref","first-page":"1525","DOI":"10.1002\/art.39072","article-title":"ERAP1 gene expression is influenced by nonsynonymous polymorphisms associated with predisposition to spondyloarthritis","volume":"67","author":"Costantino","year":"2015","journal-title":"Arthritis Rheumatol"},{"key":"2025090313003562000_6.2.e001250.29","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.imlet.2018.11.002","article-title":"Association analysis of ERAP1 gene single nucleotide polymorphism in susceptibility to ankylosing spondylitis in Iranian population","volume":"201","author":"Babaie","year":"2018","journal-title":"Immunol Lett"},{"key":"2025090313003562000_6.2.e001250.30","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1111\/cei.13121","article-title":"ERAP1\/ERAP2 and RUNX3 polymorphisms are not associated with ankylosing spondylitis susceptibility in Chinese Han","volume":"193","author":"Su","year":"2018","journal-title":"Clin Exp Immunol"},{"key":"2025090313003562000_6.2.e001250.31","doi-asserted-by":"crossref","first-page":"98","DOI":"10.1002\/mgg3.13","article-title":"A novel ERAP2 haplotype structure in a chilean population: implications for ERAP2 protein expression and preeclampsia risk","volume":"1","author":"Vanhille","year":"2013","journal-title":"Mol Genet Genomic Med"},{"key":"2025090313003562000_6.2.e001250.32","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1200918"},{"key":"2025090313003562000_6.2.e001250.33","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1002\/art.38249","article-title":"Critical role of endoplasmic reticulum aminopeptidase 1 in determining the length and sequence of peptides bound and presented by HLA-B27","volume":"66","author":"Chen","year":"2014","journal-title":"Arthritis Rheumatol"},{"key":"2025090313003562000_6.2.e001250.34","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1002\/art.40033","article-title":"Brief report: functional interaction of endoplasmic reticulum aminopeptidase 2 and HLA-B27 activates the unfolded protein response","volume":"69","author":"Zhang","year":"2017","journal-title":"Arthritis Rheumatol"},{"key":"2025090313003562000_6.2.e001250.35","doi-asserted-by":"publisher","DOI":"10.1002\/art.22295"},{"key":"2025090313003562000_6.2.e001250.36","first-page":"466","article-title":"HLA antigens in seronegative spondylarthropathies. Reactive arthritis and arthritis in ankylosing spondylitis: Relation to gut inflammation","volume":"14","author":"Mielants","year":"1987","journal-title":"J Rheumatol"},{"key":"2025090313003562000_6.2.e001250.37","first-page":"208","article-title":"MHC class I and class II genes in Tunisian patients with reactive and undifferentiated arthritis","volume":"27","author":"Siala","year":"2009","journal-title":"Clin Exp Rheumatol"},{"key":"2025090313003562000_6.2.e001250.38","doi-asserted-by":"publisher","DOI":"10.1136\/ard.61.8.714"}],"container-title":["RMD Open"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/rmdopen-2020-001250","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T20:00:45Z","timestamp":1756929645000},"score":1,"resource":{"primary":{"URL":"https:\/\/rmdopen.bmj.com\/lookup\/doi\/10.1136\/rmdopen-2020-001250"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9]]},"references-count":38,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2020,9,10]]},"published-print":{"date-parts":[[2020,9]]}},"alternative-id":["10.1136\/rmdopen-2020-001250"],"URL":"https:\/\/doi.org\/10.1136\/rmdopen-2020-001250","relation":{},"ISSN":["2056-5933"],"issn-type":[{"value":"2056-5933","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9]]}}}