{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T23:16:33Z","timestamp":1778886993994,"version":"3.51.4"},"reference-count":15,"publisher":"BMJ","issue":"2","license":[{"start":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T00:00:00Z","timestamp":1749772800000},"content-version":"unspecified","delay-in-days":73,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001826","name":"ZonMw","doi-asserted-by":"publisher","award":["09150172110053"],"award-info":[{"award-number":["09150172110053"]}],"id":[{"id":"10.13039\/501100001826","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["RMD Open"],"accepted":{"date-parts":[[2025,5,5]]},"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>Autoantibodies are a key feature of rheumatoid arthritis (RA). They can be detected years before disease onset, but it is unknown if there is any pattern in the co-occurrence of antigen recognition or isotype profiles. A common signature could point to a unique initial trigger for autoantibody development. Therefore, we sought to determine if there is a pattern in antigen or isotype reactivity in pre-symptomatic cases and established RA.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>One pre-symptomatic cohort and one RA cohort were analysed for the co-occurrence of different isotypes of anti-modified protein antibodies (AMPA) and rheumatoid factor (RF). Patterns in autoantibody levels were investigated with clustering. Additionally, total IgG was measured in 1- year follow-up sera of a representative subgroup of the RA cohort.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>While especially anti-citrullinated protein antibodies (ACPA) IgG and RF IgA co-occurred with other autoantibodies, no specific patterns emerged. In both cohorts, clusters of autoantibody levels were not determined by particular antigen reactivities or isotype. However, clusters were driven by elevated levels of several different AMPA, with distinct AMPA high- and low-level clusters. A broad IgG autoantibody profile was not accompanied by high total IgG levels.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>Autoantibody clusters are most likely not driven by AMPA specificity or isotype profile, neither before nor at RA onset, but are instead determined by a broad variety of autoantibodies. This indicates that the triggers for autoantibody development in RA do not skew the response towards certain autoreactivities or isotypes but rather lead to a broad and diverse autoantibody repertoire reflecting continuous and ongoing immune activation.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1136\/rmdopen-2024-005291","type":"journal-article","created":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T07:05:12Z","timestamp":1749798312000},"page":"e005291","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":2,"title":["Autoantibody clusters in rheumatoid arthritis are not driven by antigen specificity or isotype"],"prefix":"10.1136","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3811-7373","authenticated-orcid":false,"given":"Anouk G","family":"van Mourik","sequence":"first","affiliation":[{"name":"Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda","family":"Johansson","sequence":"additional","affiliation":[{"name":"Department of Public Health and Clinical Medicine\/Rheumatology, Ume\u00e5 University, Umea, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3101-0530","authenticated-orcid":false,"given":"Tineke J","family":"van Wesemael","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc P","family":"Maurits","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heidi","family":"Kokkonen","sequence":"additional","affiliation":[{"name":"Department of Public Health and Clinical Medicine\/Rheumatology, Ume\u00e5 University, Umea, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1186-3226","authenticated-orcid":false,"given":"Johan","family":"R\u00f6nnelid","sequence":"additional","affiliation":[{"name":"Department Immunology, Genetics and Pathology, Uppsala University, Uppsala, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7494-3023","authenticated-orcid":false,"given":"Rachel","family":"Knevel","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9618-6414","authenticated-orcid":false,"given":"Ren\u00e9 E M","family":"Toes","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8259-3863","authenticated-orcid":false,"given":"Solbritt","family":"Rantap\u00e4\u00e4-Dahlqvist","sequence":"additional","affiliation":[{"name":"Department of Public Health and Clinical Medicine\/Rheumatology, Ume\u00e5 University, Umea, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8121-5879","authenticated-orcid":false,"given":"Diane","family":"van der Woude","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Leiden University Medical Center, Leiden, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2025,6,13]]},"reference":[{"key":"2025090312041678000_11.2.e005291.1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2013-203947"},{"key":"2025090312041678000_11.2.e005291.2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200008)43:8<1831::AID-ANR19>3.0.CO;2-6"},{"key":"2025090312041678000_11.2.e005291.3","doi-asserted-by":"publisher","DOI":"10.1002\/art.30537"},{"key":"2025090312041678000_11.2.e005291.4","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1002\/art.41091","article-title":"Timing of Elevations of Autoantibody Isotypes Prior to Diagnosis of Rheumatoid Arthritis","volume":"72","author":"Kelmenson","year":"2020","journal-title":"Arthritis Rheumatol"},{"key":"2025090312041678000_11.2.e005291.5","doi-asserted-by":"crossref","DOI":"10.1186\/s13075-018-1520-4","article-title":"Baseline autoantibody profile in rheumatoid arthritis is associated with early treatment response but not long-term outcomes","volume":"20","author":"de Moel","year":"2018","journal-title":"Arthritis Res Ther"},{"key":"2025090312041678000_11.2.e005291.6","doi-asserted-by":"publisher","DOI":"10.1038\/s41584-018-0070-0"},{"key":"2025090312041678000_11.2.e005291.7","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keq230"},{"key":"2025090312041678000_11.2.e005291.8","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780310302"},{"key":"2025090312041678000_11.2.e005291.9","doi-asserted-by":"publisher","DOI":"10.1002\/art.11223"},{"key":"2025090312041678000_11.2.e005291.10","doi-asserted-by":"crossref","DOI":"10.1186\/ar3237","article-title":"Antibodies of IgG, IgA and IgM isotypes against cyclic citrullinated peptide precede the development of rheumatoid arthritis","volume":"13","author":"Kokkonen","year":"2011","journal-title":"Arthritis Res Ther"},{"key":"2025090312041678000_11.2.e005291.11","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.05.047"},{"key":"2025090312041678000_11.2.e005291.12","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2016-209794"},{"key":"2025090312041678000_11.2.e005291.13","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20022031"},{"key":"2025090312041678000_11.2.e005291.14","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2019-216499"},{"key":"2025090312041678000_11.2.e005291.15","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2010.135509"}],"container-title":["RMD Open"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/rmdopen-2024-005291","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,3]],"date-time":"2025-09-03T19:04:21Z","timestamp":1756926261000},"score":1,"resource":{"primary":{"URL":"https:\/\/rmdopen.bmj.com\/lookup\/doi\/10.1136\/rmdopen-2024-005291"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4]]},"references-count":15,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,6,13]]},"published-print":{"date-parts":[[2025,4]]}},"alternative-id":["10.1136\/rmdopen-2024-005291"],"URL":"https:\/\/doi.org\/10.1136\/rmdopen-2024-005291","relation":{},"ISSN":["2056-5933"],"issn-type":[{"value":"2056-5933","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4]]}}}