{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:04:55Z","timestamp":1790665495992,"version":"4.1.0"},"reference-count":10,"publisher":"BMJ","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sex Transm Infect"],"accepted":{"date-parts":[[2011,5,1]]},"published-print":{"date-parts":[[2011,8]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>A single 1 g dose regimen of azithromycin has been recommended for the treatment of<jats:italic>Mycoplasma genitalium<\/jats:italic>infections. The authors evaluated whether this regimen could select<jats:italic>M genitalium<\/jats:italic>strains with macrolide resistance after treatment for<jats:italic>M genitalium<\/jats:italic>-positive non-gonococcal urethritis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In seven men with non-gonococcal urethritis, who were infected with<jats:italic>M genitalium<\/jats:italic>without macrolide resistance-associated mutations but experienced microbiological azithromycin treatment failure,<jats:italic>M genitalium<\/jats:italic>DNAs in their post-treatment urine specimens were examined for mutations in the 23S rRNA gene and the ribosomal protein genes of L4 and L22. To assess the relatedness of<jats:italic>M genitalium<\/jats:italic>strains before and after treatment, their DNAs in pretreatment and post-treatment urine were genotyped by analysing short tandem repeats of an AGT\/AAT unit in the MG309 gene and single nucleotide polymorphisms in the MG191 gene.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In four of seven patients,<jats:italic>M genitalium<\/jats:italic>in post-treatment urine had an A-to-G transition at nucleotide position 2071 or 2072, corresponding to 2058 or 2059 in the 23S rRNA gene of<jats:italic>Escherichia coli<\/jats:italic>. In one of the four strains, Pro81Ser in the ribosomal protein L4 accompanied the mutation in the 23S rRNA gene. The genotyping of<jats:italic>M genitalium<\/jats:italic>DNAs suggested that these four post-treatment strains were selected from the respective closely related or identical pretreatment strains without macrolide resistance-associated mutations by the treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The single 1 g dose treatment of azithromycin could select<jats:italic>M genitalium<\/jats:italic>strains harbouring macrolide resistance-associated mutations. For<jats:italic>M genitalium<\/jats:italic>, this regimen might increase the risk of macrolide resistance selection after treatment.<\/jats:p><\/jats:sec>","DOI":"10.1136\/sextrans-2011-050035","type":"journal-article","created":{"date-parts":[[2011,6,9]],"date-time":"2011-06-09T05:24:24Z","timestamp":1307597064000},"page":"412-414","source":"Crossref","is-referenced-by-count":65,"title":["Selection of<i>Mycoplasma genitalium<\/i>strains harbouring macrolide resistance-associated 23S rRNA mutations by treatment with a single 1 g dose of azithromycin: Table 1"],"prefix":"10.1136","volume":"87","author":[{"given":"Shin","family":"Ito","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasushi","family":"Shimada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuko","family":"Yamaguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuru","family":"Yasuda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeaki","family":"Yokoi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin-ichi","family":"Ito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiro","family":"Nakano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroaki","family":"Ishiko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Deguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2011,6,8]]},"reference":[{"key":"2024051319020118000_87.5.412.1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)65268-8"},{"key":"2024051319020118000_87.5.412.2","doi-asserted-by":"publisher","DOI":"10.1086\/599033"},{"key":"2024051319020118000_87.5.412.3","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.3201\/eid1207.051558","article-title":"Azithromycin failure in Mycoplasma genitalium urethritis","volume":"12","author":"Bradshaw","year":"2006","journal-title":"Emerg Infect Dis"},{"key":"2024051319020118000_87.5.412.4","doi-asserted-by":"publisher","DOI":"10.1086\/593188"},{"key":"2024051319020118000_87.5.412.5","doi-asserted-by":"publisher","DOI":"10.3201\/eid1706.101055"},{"key":"2024051319020118000_87.5.412.6","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-130"},{"key":"2024051319020118000_87.5.412.7","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.1.1-12.2001"},{"key":"2024051319020118000_87.5.412.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2008.12.064"},{"key":"2024051319020118000_87.5.412.9","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.3.1017-1022.2003"},{"key":"2024051319020118000_87.5.412.10","doi-asserted-by":"crossref","first-page":"2823","DOI":"10.1128\/AAC.43.12.2823","article-title":"Nomenclature for macrolide and macrolide-lincosamide-streptogramin B resistance determinants","volume":"43","author":"Roberts","year":"1999","journal-title":"Antimicrob Agents Chemother"}],"container-title":["Sexually Transmitted Infections"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/sextrans-2011-050035","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,14]],"date-time":"2024-05-14T02:02:15Z","timestamp":1715652135000},"score":1,"resource":{"primary":{"URL":"https:\/\/sti.bmj.com\/lookup\/doi\/10.1136\/sextrans-2011-050035"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,6,8]]},"references-count":10,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2011,7,19]]},"published-print":{"date-parts":[[2011,8]]}},"alternative-id":["10.1136\/sextrans-2011-050035"],"URL":"https:\/\/doi.org\/10.1136\/sextrans-2011-050035","relation":{},"ISSN":["1368-4973","1472-3263"],"issn-type":[{"value":"1368-4973","type":"print"},{"value":"1472-3263","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,6,8]]}}}