{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:59:25Z","timestamp":1782781165669,"version":"3.54.5"},"reference-count":29,"publisher":"BMJ","issue":"6","content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Sex Transm Infect"],"accepted":{"date-parts":[[2012,3,25]]},"published-print":{"date-parts":[[2012,10]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>Lymphogranuloma venereum (LGV) infections caused by<jats:italic>Chlamydia trachomatis<\/jats:italic>L types have recently emerged in Europe among HIV-positive men having sex with men. Our aim was to introduce a genotyping strategy suitable for a diagnostic laboratory using nucleic acid amplification tests (NAATs) for detection of<jats:italic>C trachomatis<\/jats:italic>and to investigate the prevalence of LGV types in rectal and pharyngeal specimens in Finland.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Aptima Combo 2 (Gen-Probe) was used to detect<jats:italic>C trachomatis<\/jats:italic>in swabs. Altogether 140<jats:italic>C trachomatis<\/jats:italic>NAAT-positive rectal and pharyngeal samples were genotyped by<jats:italic>pmpH<\/jats:italic>and<jats:italic>ompA<\/jats:italic>real-time PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of the 140 NAAT-positive rectal and pharyngeal specimens, 114 (81%) were successfully typed by<jats:italic>pmpH<\/jats:italic>PCR. One hundred and four samples contained non-LGV, nine samples LGV and one sample both non-LGV and LGV<jats:italic>C trachomatis<\/jats:italic>types. The<jats:italic>C trachomatis<\/jats:italic>LGV types were mainly found in rectal samples. Six of the L types were confirmed to be genotype L2b and two were L2 with<jats:italic>ompA<\/jats:italic>PCR and sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our experience suggests that genotyping<jats:italic>C trachomatis<\/jats:italic>by<jats:italic>pmpH<\/jats:italic>PCR can be introduced as a function of a diagnostic laboratory already using NAAT for detection of<jats:italic>C trachomatis<\/jats:italic>. The data show that LGV infections occur also in Finland. LGV should be taken into account when considering treatment and management of rectal<jats:italic>C trachomatis<\/jats:italic>infections.<\/jats:p><\/jats:sec>","DOI":"10.1136\/sextrans-2011-050458","type":"journal-article","created":{"date-parts":[[2012,4,20]],"date-time":"2012-04-20T02:00:41Z","timestamp":1334887241000},"page":"465-469","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":36,"title":["Genotyping of<i>Chlamydia trachomatis<\/i>in rectal and pharyngeal specimens: identification of LGV genotypes in Finland"],"prefix":"10.1136","volume":"88","author":[{"given":"Suvi","family":"Korhonen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eija","family":"Hiltunen-Back","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirja","family":"Puolakkainen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2012,4,19]]},"reference":[{"key":"2024051321024618000_88.6.465.1","doi-asserted-by":"publisher","DOI":"10.1086\/423966"},{"key":"2024051321024618000_88.6.465.2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-010-0959-2"},{"key":"2024051321024618000_88.6.465.3","first-page":"79","volume":"57","author":"Olin","year":"1941","journal-title":"Katsaus sukupuolitautien levinneisyyteen Suomessa (in Finnish)"},{"key":"2024051321024618000_88.6.465.4","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1128\/JCM.36.2.345-351.1998","article-title":"Serotyping and genotyping of genital Chlamydia trachomatis isolates reveal variants of serovars Ba, G, and J as confirmed by omp1 nucleotide sequence analysis","volume":"36","author":"Morr\u00e9","year":"1998","journal-title":"J Clin Microbiol"},{"key":"2024051321024618000_88.6.465.5","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2008.030148"},{"key":"2024051321024618000_88.6.465.6","doi-asserted-by":"crossref","first-page":"481890","DOI":"10.1155\/2011\/481890","article-title":"Chlamydia trachomatis genotypes and the Swedish new variant among urogenital Chlamydia trachomatis strains in Finland","volume":"2011","author":"Niemi","year":"2011","journal-title":"Infect Dis Obstet Gynecol"},{"key":"2024051321024618000_88.6.465.7","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2007.029058"},{"key":"2024051321024618000_88.6.465.8","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.3.1200-1208.2003"},{"key":"2024051321024618000_88.6.465.9","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00451-07"},{"key":"2024051321024618000_88.6.465.10","doi-asserted-by":"crossref","first-page":"1489","DOI":"10.1128\/JCM.36.6.1489-1493.1998","article-title":"Comparison of performances of two commercially available tests, a PCR assay and a ligase chain reaction test, in detection of urogenital Chlamydia trachomatis infection","volume":"36","author":"Puolakkainen","year":"1998","journal-title":"J Clin Microbiol"},{"key":"2024051321024618000_88.6.465.11","first-page":"1090","article-title":"New Lymphogranuloma venereum Chlamydia trachomatis variant, Amsterdam","volume":"11","author":"Spaargaren","year":"2005","journal-title":"Emerg Infect Dis"},{"key":"2024051321024618000_88.6.465.12","unstructured":"The Statistical Database of the Infectious Diseases Register. http:\/\/www3.ktl.fi\/"},{"key":"2024051321024618000_88.6.465.13","first-page":"pii:19428","article-title":"European Surveillance of Sexually Transmitted Infections (ESSTI) network. 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