{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T05:42:01Z","timestamp":1788586921752,"version":"build-2803163510"},"reference-count":10,"publisher":"BMJ","issue":"5","content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Sex Transm Infect"],"accepted":{"date-parts":[[2012,11,11]]},"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>This article reports the fortuitous recovery of nine<jats:italic>Chlamydia trachomatis<\/jats:italic>serovar L strains in cell cultures (Vero and LLC-MK<jats:sub>2<\/jats:sub>cell line) designed for viral culture.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Nine ano-genital swabs were inoculated on confluent Vero, MRC5 and LLC-MK<jats:sub>2<\/jats:sub>cell cultures. They were collected from HIV-positive patients who were primarily men who have sex with men (MSM) presenting ulcerations that mimicked herpes simplex infections.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A cytopathogenic effect was observed on Vero and LLC-MK<jats:sub>2<\/jats:sub>cells on day 14. The presence of<jats:italic>C trachomatis<\/jats:italic>serovar L in the cell lines was confirmed by Real Time\u2013PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:italic>C trachomatis<\/jats:italic>serovar L can grow on Vero and LLC-MK<jats:sub>2<\/jats:sub>cell lines designed for viral cultures. Lymphogranuloma venereum must be considered as a differential diagnosis for herpes-like lesions, particularly in MSM with high-risk behaviours.<\/jats:p><\/jats:sec>","DOI":"10.1136\/sextrans-2012-050785","type":"journal-article","created":{"date-parts":[[2012,12,8]],"date-time":"2012-12-08T07:19:18Z","timestamp":1354951158000},"page":"395-397","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":2,"title":["Observation of a cytopathogenic effect on cell lines used for routine viral cultures led to the diagnosis of lymphogranuloma venereum"],"prefix":"10.1136","volume":"89","author":[{"given":"Laurent","family":"Busson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tania","family":"Crucitti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"De Foor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sigi","family":"Van den Wijngaert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Vandenberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2012,12,6]]},"reference":[{"key":"2024051409591243000_89.5.395.1","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1128\/CMR.10.1.160","article-title":"Current methods of laboratory diagnosis of Chlamydia trachomatis infections","volume":"10","author":"Black","year":"1997","journal-title":"Clin Microbiol Rev"},{"key":"2024051409591243000_89.5.395.2","doi-asserted-by":"crossref","first-page":"440","DOI":"10.4269\/ajtmh.1963.12.440","article-title":"The nomenclature of isolates of viruses from trachoma and inclusion blenorrhea","volume":"12","author":"Gear","year":"1963","journal-title":"Am J Trop Med Hyg"},{"key":"2024051409591243000_89.5.395.3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2008.02647.x"},{"key":"2024051409591243000_89.5.395.4","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2007.029058"},{"key":"2024051409591243000_89.5.395.5","doi-asserted-by":"crossref","first-page":"19428","DOI":"10.2807\/ese.14.48.19428-en","article-title":"Lymphogranuloma venereum in Europe, 2003\u20132008","volume":"14","author":"Savage","year":"2009","journal-title":"Euro Surveill"},{"key":"2024051409591243000_89.5.395.6","doi-asserted-by":"publisher","DOI":"10.3201\/eid1111.050821"},{"key":"2024051409591243000_89.5.395.7","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-010-0959-2"},{"key":"2024051409591243000_89.5.395.8","doi-asserted-by":"publisher","DOI":"10.1097\/01.olq.0000168429.13282.c8"},{"key":"2024051409591243000_89.5.395.9","doi-asserted-by":"crossref","first-page":"2062","DOI":"10.1128\/JCM.38.6.2062-2064.2000","article-title":"Isolation in endothelial cell cultures of Chlamydia trachomatis LGV (serovar L2) from a lymph node of a patient with suspected cat scratch disease","volume":"38","author":"Maurin","year":"2000","journal-title":"J Clin Microbiol"},{"key":"2024051409591243000_89.5.395.10","unstructured":"European Centre for Disease Prevention and Control. Sexually transmitted infections in Europe 1990\u20132010. Stockholm: ECDC, 2012. http:\/\/ecdc.europa.eu\/en\/publications\/Publications\/201206-Sexually-Transmitted-Infections-Europe-2010.pdf (accessed 9 Jul 2012)."}],"container-title":["Sexually Transmitted Infections"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/sextrans-2012-050785","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,14]],"date-time":"2024-05-14T16:59:49Z","timestamp":1715705989000},"score":1,"resource":{"primary":{"URL":"https:\/\/sti.bmj.com\/lookup\/doi\/10.1136\/sextrans-2012-050785"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12,6]]},"references-count":10,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2013,7,15]]},"published-print":{"date-parts":[[2013,8]]}},"alternative-id":["10.1136\/sextrans-2012-050785"],"URL":"https:\/\/doi.org\/10.1136\/sextrans-2012-050785","relation":{},"ISSN":["1368-4973","1472-3263"],"issn-type":[{"value":"1368-4973","type":"print"},{"value":"1472-3263","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,12,6]]}}}