{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T10:19:52Z","timestamp":1778926792058,"version":"3.51.4"},"reference-count":12,"publisher":"BMJ","issue":"6","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Sex Transm Infect"],"accepted":{"date-parts":[[2013,3,26]]},"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p><jats:italic>Trichomonas vaginalis<\/jats:italic>infection is the most prevalent treatable sexually transmitted infection (STI) in the world. An accurate point-of-care (PoC) molecular test would enable patients to be tested and treated for<jats:italic>T vaginalis<\/jats:italic>in a single visit to the genitourinary medicine clinic, community STI clinic, pharmacy or doctor's office. In this report, we describe a rapid prototype assay for<jats:italic>T vaginalis<\/jats:italic>designed for use in conjunction with the Atlas io PoC platform, and initial verification of its performance using 90 clinical samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A rapid prototype<jats:italic>T vaginalis<\/jats:italic>assay was designed. The test, featuring novel electrochemical end-point detection, used a multi-copy region of the<jats:italic>T vaginalis<\/jats:italic>genome as the assay target. Ninety clinical vaginal swab samples were used to verify the performance of the prototype assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The assay demonstrated a sensitivity and specificity of 95.5% (42\/44) and 95.7% (44\/46), respectively, when tested using clinical samples. Assay inclusivity was demonstrated for a number of geographically diverse<jats:italic>T vaginalis<\/jats:italic>isolates, and the test showed no cross-reactivity with either human DNA or a panel of DNAs isolated from common cross-reactants.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The sensitivity and specificity achieved using this prototype assay is comparable with that achieved for existing central laboratory nucleic acid amplification tests used for screening patients for<jats:italic>T vaginalis<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1136\/sextrans-2012-051000","type":"journal-article","created":{"date-parts":[[2013,4,21]],"date-time":"2013-04-21T00:21:10Z","timestamp":1366503670000},"page":"495-497","source":"Crossref","is-referenced-by-count":33,"title":["A new rapid molecular point-of-care assay for<i>Trichomonas vaginali<\/i>s: preliminary performance data"],"prefix":"10.1136","volume":"89","author":[{"given":"David M","family":"Pearce","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David N","family":"Styles","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Justin P","family":"Hardick","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charlotte A","family":"Gaydos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2013,4,20]]},"reference":[{"key":"2024051410072422000_89.6.495.1","unstructured":"World Health Organisation (WHO). Global prevalence and incidence of selected sexually transmitted infections Chlamydia trachomatis, Neisseria gonorrhoeae, syphilis and Trichomonas vaginalis: methods and results used by WHO to generate 2005 estimates. Geneva: WHO, 2011."},{"key":"2024051410072422000_89.6.495.2","doi-asserted-by":"publisher","DOI":"10.1086\/511278"},{"key":"2024051410072422000_89.6.495.3","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-199707000-00008"},{"key":"2024051410072422000_89.6.495.4","first-page":"1","article-title":"Sexually transmitted diseases treatment guidelines, 2010","volume":"59","author":"Centers for Disease Control and Prevention","year":"2010","journal-title":"MMWR Recomm Rep"},{"key":"2024051410072422000_89.6.495.5","doi-asserted-by":"publisher","DOI":"10.1086\/518851"},{"key":"2024051410072422000_89.6.495.6","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2011-050355"},{"key":"2024051410072422000_89.6.495.7","doi-asserted-by":"publisher","DOI":"10.1109\/TBME.2010.2095851"},{"key":"2024051410072422000_89.6.495.8","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02367-10"},{"key":"2024051410072422000_89.6.495.9","doi-asserted-by":"publisher","DOI":"10.1126\/science.1132894"},{"key":"2024051410072422000_89.6.495.10","doi-asserted-by":"publisher","DOI":"10.1016\/j.elecom.2004.09.019"},{"key":"2024051410072422000_89.6.495.11","unstructured":"Bard AJ Faulkner LR . Electrochemical methods: fundamentals and applications. 2nd edn. Hoboken, NJ: Wiley, 2001."},{"key":"2024051410072422000_89.6.495.12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0014144"}],"container-title":["Sexually Transmitted Infections"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/sextrans-2012-051000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,14]],"date-time":"2024-05-14T17:08:27Z","timestamp":1715706507000},"score":1,"resource":{"primary":{"URL":"https:\/\/sti.bmj.com\/lookup\/doi\/10.1136\/sextrans-2012-051000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,4,20]]},"references-count":12,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2013,8,16]]},"published-print":{"date-parts":[[2013,9]]}},"alternative-id":["10.1136\/sextrans-2012-051000"],"URL":"https:\/\/doi.org\/10.1136\/sextrans-2012-051000","relation":{},"ISSN":["1368-4973","1472-3263"],"issn-type":[{"value":"1368-4973","type":"print"},{"value":"1472-3263","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,4,20]]}}}