{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T21:14:35Z","timestamp":1761513275446},"reference-count":10,"publisher":"BMJ","issue":"7","content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Sex Transm Infect"],"accepted":{"date-parts":[[2013,3,18]]},"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>In 2006, a new variant of<jats:italic>Chlamydia trachomatis<\/jats:italic>(nvCT) was reported in Sweden. Because of a cryptic plasmid deletion, the nvCT was undetectable in several of the genetic diagnostic systems used worldwide at the time. This study aimed to evaluate whether the nvCT was present in specimens obtained from patients attending the outpatient sexually transmitted infection (STI) clinic at \u00d6rebro University Hospital, \u00d6rebro, Sweden already in 2002\u20132003.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In 2012, archival (\u221220\u00b0C freezer) urogenital specimens (2002 (n=1083) and in 2003 (n=1143)) obtained from men (2002 (n=398) and 2003 (n=486)) and women (2002 (n=301) and 2003 (n=408)) were analysed with Cobas TaqMan CT test V.2.0. All<jats:italic>C trachomatis<\/jats:italic>positive specimens were subsequently examined using a duplex PCR assay that simultaneously detects the deletion on the nvCT cryptic plasmid and the<jats:italic>ompA<\/jats:italic>gene of<jats:italic>C trachomatis<\/jats:italic>genotype E.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In total, 68 patients (9.7%) in 2002 and 61 (6.8%) in 2003 were<jats:italic>C trachomatis<\/jats:italic>positive. The duplex PCR assay identified 26<jats:italic>C trachomatis<\/jats:italic>genotype E positive patients in 2002 (38%) and 25 in 2003 (41%). No nvCT was found in 2002, but one specimen obtained from a 23-year-old man in June 2003 was positive for the nvCT.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The nvCT was present as early as 2003 in \u00d6rebro County, Sweden, which concurs with previously reported statistical estimations of its emergence. Accordingly, the nvCT spread undetected for at least 3\u2005years, explaining the high proportion (38%) in \u00d6rebro County when it was first detected in late 2006.<\/jats:p><\/jats:sec>","DOI":"10.1136\/sextrans-2013-051018","type":"journal-article","created":{"date-parts":[[2013,4,12]],"date-time":"2013-04-12T01:38:23Z","timestamp":1365730703000},"page":"607-608","update-policy":"http:\/\/dx.doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":10,"title":["The new variant of<i>Chlamydia trachomatis<\/i>was present as early as 2003 in \u00d6rebro County, Sweden, but remained undetected until 2006"],"prefix":"10.1136","volume":"89","author":[{"given":"Margaretha","family":"Jurstrand","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans","family":"Fredlund","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magnus","family":"Unemo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2013,4,11]]},"reference":[{"key":"2024051410082063000_89.7.607.1","first-page":"E061109.2","article-title":"A variant of Chlamydia trachomatis with deletion in cryptic plasmid: implications for use of PCR diagnostic tests","volume":"11","author":"Ripa","year":"2006","journal-title":"Euro Surveill"},{"key":"2024051410082063000_89.7.607.2","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0b013e32834204d5"},{"key":"2024051410082063000_89.7.607.3","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2009.040196"},{"key":"2024051410082063000_89.7.607.4","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03305.x"},{"key":"2024051410082063000_89.7.607.5","doi-asserted-by":"crossref","unstructured":"Unemo M Olc\u00e9n P Agn\u00e9-Stadling I . Experiences with the new genetic variant of Chlamydia trachomatis in \u00d6rebro county, Sweden\u2014proportion, characteristics and effective diagnostic solution in an emergent situation. Euro Surveill 2007;12 :E5\u20136.","DOI":"10.2807\/esm.12.04.00699-en"},{"key":"2024051410082063000_89.7.607.6","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2011-050409"},{"key":"2024051410082063000_89.7.607.7","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e3182593be7"},{"key":"2024051410082063000_89.7.607.8","doi-asserted-by":"publisher","DOI":"10.2340\/00015555-1269"},{"key":"2024051410082063000_89.7.607.9","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-012-0301-2"},{"key":"2024051410082063000_89.7.607.10","first-page":"19206","article-title":"Can the Swedish new variant of Chlamydia trachomatis (nvCT) be detected by UK NEQAS participants from seventeen European countries and five additional countries\/regions in 2009?","volume-title":"Euro Surveill","volume":"14","author":"Unemo","year":"2009"}],"container-title":["Sexually Transmitted Infections"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/sextrans-2013-051018","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,14]],"date-time":"2024-05-14T17:09:34Z","timestamp":1715706574000},"score":1,"resource":{"primary":{"URL":"https:\/\/sti.bmj.com\/lookup\/doi\/10.1136\/sextrans-2013-051018"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,4,11]]},"references-count":10,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2013,10,11]]},"published-print":{"date-parts":[[2013,11]]}},"alternative-id":["10.1136\/sextrans-2013-051018"],"URL":"https:\/\/doi.org\/10.1136\/sextrans-2013-051018","relation":{},"ISSN":["1368-4973","1472-3263"],"issn-type":[{"value":"1368-4973","type":"print"},{"value":"1472-3263","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,4,11]]}}}