{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T14:20:23Z","timestamp":1762006823510,"version":"build-2065373602"},"reference-count":18,"publisher":"BMJ","issue":"2","funder":[{"name":"Roche Molecular Systems, Pleasanton, CA"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Sex Transm Infect"],"accepted":{"date-parts":[[2018,7,9]]},"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>Infections due to<jats:italic>Chlamydia trachomatis<\/jats:italic>(CT) and<jats:italic>Neisseria gonorrhoeae<\/jats:italic>(NG) are among the most common bacterial sexually transmitted infections worldwide, most of which are asymptomatic. Detection of infection using a variety of specimen types in symptomatic and asymptomatic subjects is important to effectively combat CT\/NG infections. The performance of the cobas CT\/NG v2.0 test was assessed for urogenital swabs, urine and cervical cytology samples collected in PreservCyt Solution from 5266 symptomatic and asymptomatic women (including 202 who were pregnant), and urine from 738 men.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sensitivity and specificity were estimated compared with a patient infected status determined using two US Food and Drug Administration\u2013cleared nucleic acid amplification tests.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among 6004 participants, 487 CT (8.1%) and 159 NG (2.6%) infections were identified. Sensitivity estimates for CT for women ranged from 91.2% to 97.6% depending on specimen type, and the estimate for male urine specimens was 98.4%. Specificity for CT ranged from 99.2% to 99.7%. Sensitivity estimates for NG ranged from 95.6% to 100.0% for women, and the estimate for men was 100.0%. Specificity for NG ranged from 99.3% to 100.0%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The cobas CT\/NG v2.0 test performs well using urogenital swabs, urine and cervical samples collected in PreservCyt solution.<\/jats:p><\/jats:sec>","DOI":"10.1136\/sextrans-2018-053545","type":"journal-article","created":{"date-parts":[[2018,8,20]],"date-time":"2018-08-20T16:14:32Z","timestamp":1534781672000},"page":"87-93","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":12,"title":["Detection of<i>Chlamydia trachomatis<\/i>and<i>Neisseria gonorrhoeae<\/i>with the cobas CT\/NG v2.0 test: performance compared with the BD ProbeTec CT Q<sup>x<\/sup>and GC Q<sup>x<\/sup>amplified DNA and Aptima AC2 assays"],"prefix":"10.1136","volume":"95","author":[{"given":"Melinda B","family":"Nye","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Osiecki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Lewinski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliver","family":"Liesenfeld","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Young","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie N","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca A","family":"Lillis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara A","family":"Body","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carol","family":"Eisenhut","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward W","family":"Hook III","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara","family":"Van Der Pol","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2018,8,20]]},"reference":[{"key":"2024092304402082000_95.2.87.1","unstructured":"World Health Organisation. Prevalence and incidence in 2005 of selected sexually transmitted infections: methods and results. Geneva, Switzerland, 2011."},{"key":"2024092304402082000_95.2.87.2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-7435(02)00058-0"},{"key":"2024092304402082000_95.2.87.3","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e3181ec51f1"},{"key":"2024092304402082000_95.2.87.4","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-199207000-00001"},{"key":"2024092304402082000_95.2.87.5","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-147-2-200707170-00172"},{"key":"2024092304402082000_95.2.87.6","first-page":"1","article-title":"Centers for Disease Control and Prevention (CDC). Recommendations for the laboratory-based detection of Chlamydia trachomatis and Neisseria gonorrhoeae\u20142014","volume":"63","year":"2014","journal-title":"MMWR Recomm Rep"},{"key":"2024092304402082000_95.2.87.7","unstructured":"Public Health England. National chlamydia screeningprogramme standards 2016. https:\/\/assets.publishing.service.gov.uk\/government\/uploads\/system\/uploads\/attachment_data\/file\/574351\/NCSP_Standards_7th_edition.pdf (accessed May 2018)."},{"key":"2024092304402082000_95.2.87.8","unstructured":"Public Health England. Guidance for the detection of gonorrhea in England. 2015. https:\/\/assets.publishing.service.gov.uk\/government\/uploads\/system\/uploads\/attachment_data\/file\/405293\/170215_Gonorrhoea_testing_guidance_REVISED__2_.pdf (accessed May 2018)."},{"key":"2024092304402082000_95.2.87.9","doi-asserted-by":"publisher","DOI":"10.1177\/0956462415618837"},{"key":"2024092304402082000_95.2.87.10","doi-asserted-by":"publisher","DOI":"10.1177\/0956462412472837"},{"key":"2024092304402082000_95.2.87.11","doi-asserted-by":"publisher","DOI":"10.1016\/j.jadohealth.2011.11.002"},{"key":"2024092304402082000_95.2.87.12","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e31824e26ff"},{"key":"2024092304402082000_95.2.87.13","doi-asserted-by":"publisher","DOI":"10.1586\/erm.12.141"},{"key":"2024092304402082000_95.2.87.14","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06481-11"},{"key":"2024092304402082000_95.2.87.15","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e3182717833"},{"key":"2024092304402082000_95.2.87.16","unstructured":"Draft guidance for industry and Food and Drug Administration staff\u2014establishing the performance characteristics of in vitro diagnostic devices for Chlamydia trachomatis and\/or Neisseria gonorrhoea: screening and diagnostic testing. Rockville, MD: United States Department of Health and Human Services Food and Drug Administration, 2011."},{"key":"2024092304402082000_95.2.87.17","unstructured":"CLSI. User protocol for evaluation of qualitative test performance; approved guideline\u2014second edition. Wayne, PA: Clinical and Laboratory Standards Institute, 2008."},{"key":"2024092304402082000_95.2.87.18","doi-asserted-by":"publisher","DOI":"10.2307\/2276774"}],"container-title":["Sexually Transmitted Infections"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/sextrans-2018-053545","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,23]],"date-time":"2024-09-23T11:41:07Z","timestamp":1727091667000},"score":1,"resource":{"primary":{"URL":"https:\/\/sti.bmj.com\/lookup\/doi\/10.1136\/sextrans-2018-053545"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,20]]},"references-count":18,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2019,2,25]]},"published-print":{"date-parts":[[2019,3]]}},"alternative-id":["10.1136\/sextrans-2018-053545"],"URL":"https:\/\/doi.org\/10.1136\/sextrans-2018-053545","relation":{},"ISSN":["1368-4973","1472-3263"],"issn-type":[{"type":"print","value":"1368-4973"},{"type":"electronic","value":"1472-3263"}],"subject":[],"published":{"date-parts":[[2018,8,20]]}}}