{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T05:42:01Z","timestamp":1788586921204,"version":"build-2803163510"},"reference-count":10,"publisher":"BMJ","issue":"8","content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Sex Transm Infect"],"accepted":{"date-parts":[[2020,3,17]]},"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>We evaluated the prevalence of lymphogranuloma venereum (LGV) in anorectal<jats:italic>Chlamydia trachomatis<\/jats:italic>-positive French men who have sex with men (MSM) using pre-exposure prophylaxis (PrEP) for HIV. Here, we describe the clinical, biological and behavioural characteristics of these patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Laboratories throughout French metropolitan areas performing routine testing for<jats:italic>C. trachomatis<\/jats:italic>sent positive anorectal specimens to the National Reference Centre for bacterial STIs for LGV real-time PCR targeting the<jats:italic>pmp<\/jats:italic>H gene. Identification of the<jats:italic>C. trachomatis<\/jats:italic>genovar was performed by<jats:italic>omp<\/jats:italic>A gene sequencing. For each patient, clinical, biological and sexual behaviour data were collected after obtaining written informed consent.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In 2017, 486 anorectal<jats:italic>C. trachomatis<\/jats:italic>-positive specimens from MSM PrEP users were analysed. A strain of genovar L was detected in 91 cases (18.7%). Patients with LGV were significantly more symptomatic, had more sexual partners and more concurrent syphilis compared with their non-LGV counterparts.<jats:italic>Omp<\/jats:italic>A gene sequencing, successful in two-thirds of anorectal<jats:italic>C. trachomatis<\/jats:italic>-positive specimens, showed that the LGV cases were mainly of variant L2b (n=33), followed by genovar L2 (n=27) and genetic L2b<jats:italic>omp<\/jats:italic>A variants (n=16). In 11 cases, the results indicated the occurrence of genetic exchange between L and non-L genovars.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>LGV was diagnosed in 18.7% of anorectal<jats:italic>C. trachomatis<\/jats:italic>-positive specimens from French MSM using PrEP. LGV testing should be carried out for MSM diagnosed with chlamydia and with a large number of sexual partners, high-risk practices and anorectal symptoms. These patients should be presumptively treated as having LGV. This is the first surveillance study of LGV among MSM PrEP users and monitoring should continue.<\/jats:p><\/jats:sec>","DOI":"10.1136\/sextrans-2019-054346","type":"journal-article","created":{"date-parts":[[2020,4,17]],"date-time":"2020-04-17T21:16:39Z","timestamp":1587158199000},"page":"615-617","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":28,"title":["Prevalence of lymphogranuloma venereum among anorectal<i>Chlamydia trachomatis<\/i>-positive MSM using pre-exposure prophylaxis for HIV"],"prefix":"10.1136","volume":"96","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5358-8990","authenticated-orcid":false,"given":"Olivia","family":"Peuchant","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9369-4931","authenticated-orcid":false,"given":"Arabella","family":"Touati","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Laurier-Nadali\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadege","family":"H\u00e9nin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Cazanave","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"B\u00e9b\u00e9ar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bertille","family":"de Barbeyrac","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2020,4,17]]},"reference":[{"key":"2024092304051013000_96.8.615.1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciy182"},{"key":"2024092304051013000_96.8.615.2","doi-asserted-by":"crossref","first-page":"1215","DOI":"10.1177\/0956462418785266","article-title":"Observational study of anorectal Chlamydia trachomatis infections in France through the lymphogranuloma venereum surveillance network, 2010-2015","volume":"29","author":"de Barbeyrac","year":"2018","journal-title":"Int J STD AIDS"},{"key":"2024092304051013000_96.8.615.3","doi-asserted-by":"publisher","DOI":"10.3201\/eid1108.050535"},{"key":"2024092304051013000_96.8.615.4","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1128\/jcm.32.2.528-530.1994","article-title":"Improved PCR sensitivity for direct genotyping of Chlamydia trachomatis serovars by using a nested PCR","volume":"32","author":"Lan","year":"1994","journal-title":"J Clin Microbiol"},{"key":"2024092304051013000_96.8.615.5","doi-asserted-by":"crossref","first-page":"1945","DOI":"10.3201\/eid2211.160247","article-title":"Changing Pattern of Chlamydia trachomatis Strains in Lymphogranuloma Venereum Outbreak, France, 2010-2015","volume":"22","author":"Peuchant","year":"2016","journal-title":"Emerg Infect Dis"},{"key":"2024092304051013000_96.8.615.6","doi-asserted-by":"crossref","first-page":"1684","DOI":"10.1099\/jmm.0.000597","article-title":"Lymphogranuloma venereum rates increased and Chlamydia trachomatis genotypes changed among men who have sex with men in Sweden 2004-2016","volume":"66","author":"Isaksson","year":"2017","journal-title":"J Med Microbiol"},{"key":"2024092304051013000_96.8.615.7","doi-asserted-by":"crossref","first-page":"574.e7","DOI":"10.1016\/j.cmi.2016.03.018","article-title":"The L2b real-time PCR targeting the PMP H gene of Chlamydia trachomatis used for the diagnosis of lymphogranuloma venereum is not specific to L2b strains","volume":"22","author":"Touati","year":"2016","journal-title":"Clinical Microbiology and Infection"},{"key":"2024092304051013000_96.8.615.8","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2012-050915"},{"key":"2024092304051013000_96.8.615.9","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2019-053972"},{"key":"2024092304051013000_96.8.615.10","doi-asserted-by":"crossref","first-page":"1821","DOI":"10.1111\/jdv.15729","article-title":"2019 European guideline on the management of lymphogranuloma venereum","volume":"33","author":"de Vries","year":"2019","journal-title":"J Eur Acad Dermatol Venereol"}],"container-title":["Sexually Transmitted Infections"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/sextrans-2019-054346","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,23]],"date-time":"2024-09-23T11:06:15Z","timestamp":1727089575000},"score":1,"resource":{"primary":{"URL":"https:\/\/sti.bmj.com\/lookup\/doi\/10.1136\/sextrans-2019-054346"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,17]]},"references-count":10,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2020,11,18]]},"published-print":{"date-parts":[[2020,12]]}},"alternative-id":["10.1136\/sextrans-2019-054346"],"URL":"https:\/\/doi.org\/10.1136\/sextrans-2019-054346","relation":{},"ISSN":["1368-4973","1472-3263"],"issn-type":[{"value":"1368-4973","type":"print"},{"value":"1472-3263","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,17]]}}}