{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T03:44:35Z","timestamp":1772163875382,"version":"3.50.1"},"reference-count":31,"publisher":"BMJ","issue":"2","funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81401749"],"award-info":[{"award-number":["81401749"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81471231"],"award-info":[{"award-number":["81471231"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81471967"],"award-info":[{"award-number":["81471967"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81672094"],"award-info":[{"award-number":["81672094"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81771312"],"award-info":[{"award-number":["81771312"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81772260"],"award-info":[{"award-number":["81772260"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81871729"],"award-info":[{"award-number":["81871729"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Key Projects for Province Science and Technology Program of Fujian","award":["2018D0014"],"award-info":[{"award-number":["2018D0014"]}]},{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2016J01628"],"award-info":[{"award-number":["2016J01628"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Sex Transm Infect"],"accepted":{"date-parts":[[2020,10,25]]},"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      A novel\n                      <jats:italic>tp0548<\/jats:italic>\n                      sequence-type was identified in one clinical isolate (X-4) from a patient diagnosed with primary syphilis in Xiamen, China. To precisely define and characterise a new clinical isolate, we performed further genome-scale molecular analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The pooled segment genome sequencing method followed by Illumina sequencing was performed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>This novel sequence-type contained a unique nucleotide substitution \u2018T\u2019 at position 167 and belonged to the SS14-like clade of TPA strains, as determined by phylogenetic analysis. Multi-locus sequence analysis of nine chromosomal loci demonstrated that the X-4 isolate was clustered within a monophyletic group of TPA strains. Whole-genome phylogenetic analysis subsequently corroborated the TPA strain classification of the X-4 isolate and revealed that the isolate was closely related to the SS14 strain, with 42 single-nucleotide variations and 12 insertions\/deletions. In addition, high intrastrain heterogeneity in the length of the poly G\/C tracts was found in the TPAChi_0347 locus, which might indicate that this gene of the X-4 isolate is likely involved in phase variation events. The length heterogeneity of the poly A\/T tracts was lower than the genetic variability of the poly G\/C tracts, and all the observed intrastrain variations fell within coding regions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The novel\n                      <jats:italic>tp0548<\/jats:italic>\n                      sequence-type was determined to belong to a new TPA isolate, X-4. The identification of variable length in homopolymetic tracts (G\/C and A\/T) could provide a snapshot of the genes that potentially involved in genotype\u2013phenotype variations. These findings provide an unequivocal characterisation for better understanding the molecular variation of this emerging isolate.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/sextrans-2020-054687","type":"journal-article","created":{"date-parts":[[2020,11,19]],"date-time":"2020-11-19T22:46:23Z","timestamp":1605825983000},"page":"120-125","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":6,"title":["Characterisation of the novel clinical isolate X-4 containing a new\n                    <i>tp0548<\/i>\n                    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