{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T23:49:33Z","timestamp":1787701773090,"version":"build-2784847793"},"reference-count":10,"publisher":"BMJ","issue":"3","content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Sex Transm Infect"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>This study sought to provide data on the prevalence of macrolide (23S rRNA) and fluoroquinolone (<jats:italic>parC<\/jats:italic>) resistance-associated mutations seen in <jats:italic>Mycoplasma genitalium<\/jats:italic>-positive specimens received in the UK national reference laboratory.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In total, 2580 clinical specimens from patients with suspected or confirmed <jats:italic>M. genitalium<\/jats:italic> infection were received at the national reference laboratory between September 2017 and November 2018. <jats:italic>M. genitalium<\/jats:italic>-positive clinical specimens were identified using a reverse transcription-PCR targeting two <jats:italic>M. genitalium<\/jats:italic> genes: <jats:italic>MgPa<\/jats:italic> and <jats:italic>gap<\/jats:italic>. Resistance-associated single nucleotide poylmorphisms were sought in all positive specimens by sequence analysis of the 23S rRNA and <jats:italic>parC<\/jats:italic> genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Eighteen per cent (458 of 2580) of clinical specimens were positive for <jats:italic>M. genitalium<\/jats:italic> and 389 had sequence data for both macrolide and fluoroquinolone resistance markers. Of these, 71% (275 of 389) had macrolide resistance-associated mutations, 8% (31 of 389) had fluoroquinolone resistance-associated mutations (S83I\/R and D87Y\/N) and 7% (26 of 389) had mutations associated with resistance to both antimicrobials. Only 28% (108 of 389) had no mutations associated with resistance to either class of antibiotic. Five specimens had mutations of unknown clinical significance in the <jats:italic>parC<\/jats:italic> gene (eg, G81C and S83N).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Mutations associated with resistance to macrolides were very frequent. By contrast, susceptibility to the second-line treatment, moxifloxacin (a fluoroquinolone), was estimated at 92% based on the absence of resistance-associated mutations. The few specimens with mutations of unknown clinical significance in the <jats:italic>parC<\/jats:italic> gene were excluded from the analysis and so the actual level of fluoroquinolone susceptibility may be slightly lower than that reported here. Surveillance of antimicrobial resistance in <jats:italic>M. genitalium<\/jats:italic> is imperative for this to remain a treatable infection.<\/jats:p><\/jats:sec>","DOI":"10.1136\/sextrans-2020-054897","type":"journal-article","created":{"date-parts":[[2021,6,4]],"date-time":"2021-06-04T17:32:23Z","timestamp":1622827943000},"page":"215-218","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":6,"title":["Detection of markers predictive of macrolide and fluoroquinolone resistance in <i>Mycoplasma genitalium<\/i> from patients attending sexual health services"],"prefix":"10.1136","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5089-2321","authenticated-orcid":false,"given":"Michaela Joanne","family":"Day","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6707-6910","authenticated-orcid":false,"given":"Michelle Jayne","family":"Cole","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7756-403X","authenticated-orcid":false,"given":"Helen","family":"Fifer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil","family":"Woodford","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3524-5935","authenticated-orcid":false,"given":"Rachel","family":"Pitt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2021,6,4]]},"reference":[{"key":"2022100410401129000_98.3.215.1","doi-asserted-by":"publisher","DOI":"10.1177\/0956462413502008"},{"key":"2022100410401129000_98.3.215.2","doi-asserted-by":"crossref","unstructured":"Soni S , Horner P , Rayment M . British Association for Sexual Health and HIV national guideline for the management of infection with Mycoplasma genitalium, 2018. Available: https:\/\/www.bashhguidelines.org\/media\/1198\/mg-2018.pdf","DOI":"10.1177\/0956462419825948"},{"key":"2022100410401129000_98.3.215.3","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e31819e3f7a"},{"key":"2022100410401129000_98.3.215.4","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/48.5.742"},{"key":"2022100410401129000_98.3.215.5","doi-asserted-by":"publisher","DOI":"10.3201\/eid2305.161745"},{"key":"2022100410401129000_98.3.215.6","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.2.683-692.2004"},{"key":"2022100410401129000_98.3.215.7","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.7.3121-3128.2005"},{"key":"2022100410401129000_98.3.215.8","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2017-053164"},{"key":"2022100410401129000_98.3.215.9","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01956-12"},{"key":"2022100410401129000_98.3.215.10","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0198355"}],"container-title":["Sexually Transmitted Infections"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/sextrans-2020-054897","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,10,4]],"date-time":"2022-10-04T17:40:56Z","timestamp":1664905256000},"score":1,"resource":{"primary":{"URL":"https:\/\/sti.bmj.com\/lookup\/doi\/10.1136\/sextrans-2020-054897"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,4]]},"references-count":10,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2022,4,12]]},"published-print":{"date-parts":[[2022,5]]}},"alternative-id":["10.1136\/sextrans-2020-054897"],"URL":"https:\/\/doi.org\/10.1136\/sextrans-2020-054897","relation":{},"ISSN":["1368-4973","1472-3263"],"issn-type":[{"value":"1368-4973","type":"print"},{"value":"1472-3263","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,4]]}}}