{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T08:24:32Z","timestamp":1750062272257,"version":"3.37.3"},"reference-count":9,"publisher":"BMJ","issue":"1","funder":[{"name":"Australian Government Research Training Program","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["APP1174555"],"award-info":[{"award-number":["APP1174555"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"crossref","award":["IH190100021"],"award-info":[{"award-number":["IH190100021"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Sex Transm Infect"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>To ensure accurate diagnosis of infectious diseases, preanalytical factors should be considered when assessing specimen quality and subsequent test result. Accordingly, we aimed to systematically assess the effect of storage time, temperature and buffer on the analytical sensitivity of detecting the sexually transmitted pathogen,<jats:italic>Neisseria gonorrhoeae<\/jats:italic>across multiple molecular diagnostic platforms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cultured<jats:italic>N. gonorrhoeae<\/jats:italic>was spiked into generic and commercial storage buffers and stored at four temperatures and five time points, ranging from \u221220\u00b0C to 37\u00b0C, over 30 days. Samples were processed using the Alinity m STI, Xpert CT\/NG and Aptima Combo 2 nucleic acid amplification assays and an in-house quantitative PCR assay. A reduction in analytical sensitivity was defined as a significant (p&lt;0.05) increase in cycle threshold (Ct) value relative to control samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In total, 2756 samples were processed, with<jats:italic>N. gonorrhoeae<\/jats:italic>detected in 99.2% of samples. With respect to time, analytical sensitivity was maintained from day 2 (113\/120; 94.2%) up to day 30 (110\/120; 91.7%) relative to baseline samples. With respect to temperature, analytical sensitivity was maintained from \u221220\u00b0C (147\/150; 98.0%) up to 37\u00b0C (136\/150; 90.7%) relative to baseline samples. Generic buffers, Viral Transport Medium and Amies Liquid Media showed a reduction in analytical sensitivity compared with their commercial counterparts, Aptima Multitest Swab Transport Media and Abbott Alinity transport buffer using select diagnostic assays; this reduction appeared temperature dependent, with the largest differences in median Ct values observed at 37\u00b0C (p&lt;0.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Increased prevalence of sample self-collection for sexually transmitted infections (STIs) warrants an evaluation of preanalytical sample storage variables on diagnostic testing performance. Here, across a range of time points, temperatures and storage buffers,<jats:italic>N. gonorrhoeae<\/jats:italic>was successfully detected, supporting flexibility in sample storage, and by extension the feasibility of analysing self-collected samples to improve access to STI testing.<\/jats:p><\/jats:sec>","DOI":"10.1136\/sextrans-2023-055859","type":"journal-article","created":{"date-parts":[[2023,11,24]],"date-time":"2023-11-24T18:21:17Z","timestamp":1700850077000},"page":"48-51","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":2,"title":["Evaluating preanalytical sample storage parameters for nucleic acid-based detection of<i>Neisseria gonorrhoeae<\/i>"],"prefix":"10.1136","volume":"100","author":[{"given":"Janath A","family":"Fernando","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelina","family":"Krysiak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacqueline","family":"Prestedge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca","family":"Azzato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7363-6665","authenticated-orcid":false,"given":"Deborah A","family":"Williamson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9681-1956","authenticated-orcid":false,"given":"Shivani","family":"Pasricha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"239","published-online":{"date-parts":[[2023,11,24]]},"reference":[{"key":"2024021320200844000_100.1.48.1","unstructured":"WHO . Sexually transmitted infections (STIs). Geneva WHO Press; 2021. Available: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/sexually-transmitted-infections-(stis) [Accessed 22 Aug 2022]."},{"key":"2024021320200844000_100.1.48.2","doi-asserted-by":"crossref","first-page":"S26","DOI":"10.1089\/pop.2020.0296","article-title":"Evolution of specimen self-collection in the COVID-19 era: implications for population health management of infectious disease","volume":"24","author":"Cockerill","year":"2021","journal-title":"Popul Health Manag"},{"key":"2024021320200844000_100.1.48.3","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1071\/SH16117","article-title":"Uriswab: an effective transport medium for nucleic acid detection of chlamydia trachomatis, mycoplasma genitalium and neisseria gonorrhoeae","volume":"14","author":"Costa","year":"2017","journal-title":"Sex Health"},{"key":"2024021320200844000_100.1.48.4","doi-asserted-by":"crossref","first-page":"105719","DOI":"10.1016\/j.mimet.2019.105719","article-title":"Impact of specimen storage temperature and time on the implementation of Genexpert\u00ae testing for sexually transmitted infections in resource-constraint settings","volume":"165","author":"Maduna","year":"2019","journal-title":"J Microbiol Methods"},{"key":"2024021320200844000_100.1.48.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2007.12.007"},{"key":"2024021320200844000_100.1.48.6","doi-asserted-by":"publisher","DOI":"10.1093\/bjaceaccp\/mkn041"},{"key":"2024021320200844000_100.1.48.7","doi-asserted-by":"crossref","unstructured":"Dennis TPW , Mable BK , Brunelle B , et al . Target-enrichment sequencing yields valuable genomic data for difficult-to-culture bacteria of public health importance. Molecular Biology [Preprint] 2022. doi:10.1101\/2022.02.16.480634","DOI":"10.1101\/2022.02.16.480634"},{"key":"2024021320200844000_100.1.48.8","doi-asserted-by":"crossref","unstructured":"Bachmann NL , Rockett RJ , Timms VJ , et al . Advances in clinical sample preparation for identification and characterization of bacterial pathogens using metagenomics. Front Public Health 2018;6:363. doi:10.3389\/fpubh.2018.00363","DOI":"10.3389\/fpubh.2018.00363"},{"key":"2024021320200844000_100.1.48.9","doi-asserted-by":"crossref","unstructured":"van der Veer BMJW , Hoebe CJPA , Dukers-Muijrers NHTM , et al . Men and women have similar Neisseria Gonorrhoeae bacterial loads: a comparison of three anatomical sites. J Clin Microbiol 2020;58:e01171-20. doi:10.1128\/JCM.01171-20","DOI":"10.1128\/JCM.01171-20"}],"container-title":["Sexually Transmitted Infections"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/sextrans-2023-055859","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,14]],"date-time":"2024-02-14T04:20:32Z","timestamp":1707884432000},"score":1,"resource":{"primary":{"URL":"https:\/\/sti.bmj.com\/lookup\/doi\/10.1136\/sextrans-2023-055859"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,24]]},"references-count":9,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,1,17]]},"published-print":{"date-parts":[[2024,2]]}},"alternative-id":["10.1136\/sextrans-2023-055859"],"URL":"https:\/\/doi.org\/10.1136\/sextrans-2023-055859","relation":{},"ISSN":["1368-4973","1472-3263"],"issn-type":[{"type":"print","value":"1368-4973"},{"type":"electronic","value":"1472-3263"}],"subject":[],"published":{"date-parts":[[2023,11,24]]}}}