{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T01:35:10Z","timestamp":1789436110934,"version":"build-2803163510"},"reference-count":25,"publisher":"BMJ","issue":"8","funder":[{"name":"Catalonia European Regional Development Fund","award":["SA51-006646"],"award-info":[{"award-number":["SA51-006646"]}]}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Sex Transm Infect"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>Culture of<jats:italic>Neisseria gonorrhoeae<\/jats:italic>remains essential for antimicrobial resistance (AMR) surveillance. We evaluated the effect of time of specimen collection on culture yield following a positive nucleic acid amplification test (NAAT).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We retrospectively assessed<jats:italic>N. gonorrhoeae<\/jats:italic>culture yield among asymptomatic individuals (largely men who have sex with men) who attended for sexual health screening and had a positive NAAT. Participants underwent either same-day testing and notification (Drassanes Expr\u00e9s) or standard screening with deferred testing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among 10\u2009423 screened individuals, 809 (7.7%) tested positive for<jats:italic>N. gonorrhoeae<\/jats:italic>. A total of 995 different anatomical sites of infection culture was performed in 583 of 995 (58.6%) of anatomical sites (Drassanes Expr\u00e9s 278 of 347, 80.1%; standard screening 305 of 648, 47.1%; p&lt;0.001). Recovery was highest when culture specimens were collected within 3\u20137\u2009days of screening with only a slight drop in recovery when the interval extended to 7\u2009days . Recovery from pharynx was 38 of 149 (25.5%) within 3\u2009days, 19 of 81 (23.4%) after 4\u20137\u2009days (p=0.7245), 11 of 102 (10.7%) after 8\u201314\u2009days (p&lt;0.0036) and 1 of 22 (4.5%) with longer delays (p=0.00287). Recovery from rectum was 49 of 75 (65.3%) within 3\u2009days, 28 of 45 (62.2%) after 4\u20137\u2009days (p=0.7318), 41 of 69 (59.4%) after 8\u201314\u2009days (p=0.4651) and 6 of 18 (33.3%) with longer delays (p=0.0131). Median culture specimen collection time was 1\u2009day within Drassanes Expr\u00e9s vs 8\u2009days within standard screening. Consequently, the overall culture yield was slightly higher within Drassanes Expr\u00e9s (102\/278, 36.6% vs 99\/305, 32.5%; p=0.2934).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Reducing the interval between screening and collection of culture specimens increased<jats:italic>N. gonorrhoeae<\/jats:italic>recovery in extragenital samples. Implementing a same-day testing and notification programme increased collection of culture samples and culture yield in our setting, which may help AMR surveillance.<\/jats:p><\/jats:sec>","DOI":"10.1136\/sextrans-2023-055899","type":"journal-article","created":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T13:50:10Z","timestamp":1700229010000},"page":"520-526","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":5,"title":["Impact of time of culture specimen collection on the recovery of<i>Neisseria gonorrhoeae<\/i>after a positive nucleic acid amplification test"],"prefix":"10.1136","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2656-2656","authenticated-orcid":false,"given":"Patricia","family":"Nadal-Bar\u00f3n","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas","family":"Trejo-Zahinos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge Nestor","family":"Garc\u00eda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula","family":"Salmer\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Sulleiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maider","family":"Arando","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vicente","family":"Descalzo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"\u00c1lvarez-Lopez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachid","family":"El Ouazzani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"L\u00f3pez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesc","family":"Zarzuela","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edurne","family":"Ruiz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Montserrat","family":"Llinas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert","family":"Blanco-Grau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian","family":"Curran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Nieves","family":"Larrosa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom\u00e0s","family":"Pumarola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yannick","family":"Hoyos-Mallecot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2023,10,6]]},"reference":[{"key":"2023111705500702000_99.8.520.1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1512757"},{"key":"2023111705500702000_99.8.520.2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00010-14"},{"key":"2023111705500702000_99.8.520.3","doi-asserted-by":"crossref","unstructured":"Meyer T , Buder S . 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