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J. (1961). J. Physiol. (Lond.), 155, 26P.","journal-title":"J. Physiol. (Lond.)"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"312","DOI":"10.4269\/ajtmh.1959.8.312","volume":"8","author":"Burch, T.A.; Rees, C.W.; Reardon, L.V.","year":"1959","unstructured":"Burch, T. A., Rees, C. W., and Reardon, L. V. (1959). Amer. J. trop. Med. Hyg., 8, 312.","journal-title":"Amer. J. trop. Med. Hyg"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"524","DOI":"10.2307\/3275830","volume":"49","author":"Collins, W.E.; Jeffery, G.M.","year":"1963","unstructured":"Collins, W. E., and Jeffery, G. M. (1963). J. Parasit., 49, 524.","journal-title":"J. Parasit"},{"key":"ref_4","article-title":"Mackie and McCartney's Handbook of Bacteriology","author":"Cruickshank, R.","year":"1960","unstructured":"Cruickshank, R. (ed.) (1960). \"Mackie and McCartney's Handbook of Bacteriology\", 10th ed. Livingstone, Edinburgh.","edition":"10th ed"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1016\/0014-4894(63)90032-8","volume":"14","author":"Cunningham, M.P.; Lumsden, W.H.R.; Webber, W.A.F.","year":"1963","unstructured":"Cunningham, M. P., Lumsden, W. H. R., and Webber, W. A. F. (1963). Exp. Parasit., 14, 280.","journal-title":"Exp. Parasit"},{"key":"ref_6","article-title":"Practical Haematology","author":"Dacie, J.V.; Lewis, S.M.","year":"1963","unstructured":"Dacie, J. V., and Lewis, S. M. (1963). \"Practical Haematology\", 3rd ed. Churchill, London.","edition":"3rd ed"},{"key":"ref_7","article-title":"Traite des entozoaires et des maladies vermineuses de 1'homme et des animaux domestiques","author":"Davaine, C.","year":"1860","unstructured":"Davaine, C. (1860). \"Traite des entozoaires et des maladies vermineuses de 1'homme et des animaux domestiques\". Bailliere, Paris. (Cited by Trussell, 1947)."},{"key":"ref_8","first-page":"464","volume":"4","author":"Donne, A.","year":"1837","unstructured":"Donne, A. (1837). C. R. Acad. Sci. (Paris), 4, 464. (Cited bv Trussell, 1947).","journal-title":"C. R. Acad. Sci. (Paris)"},{"key":"ref_9","article-title":"Documenta Geigy. Scientific Tables","author":"Diem, K.","year":"1962","unstructured":"Diem, K. (ed.) (1962). \"Documenta Geigy. Scientific Tables\", 6th ed. Geigy Pharmaceutical Co., Manchester.","edition":"6th ed."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1136\/jcp.10.4.327","volume":"10","author":"Feinberg, J.G.; Whittington, M.J.","year":"1957","unstructured":"Feinberg, J. G., and Whittington, M. J. (1957). J. clin. Path., 10, 327.","journal-title":"J. clin. Path"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1111\/j.1550-7408.1961.tb01175.x","volume":"8","author":"Fitzgerald, P.R.; Levine, N.D.","year":"1961","unstructured":"Fitzgerald, P. R., and Levine, N. D. (1961). J. Protozool., 8, 21.","journal-title":"J. Protozool"},{"key":"ref_12","first-page":"p.","article-title":"East African Trypanosomiasis Research Organization Report, 1962-63","author":"Grainge, E.B.","year":"1963","unstructured":"Grainge, E. B. (1963). \"East African Trypanosomiasis Research Organization Report, 1962-63\", p."},{"key":"ref_13","first-page":"90","volume":"22","author":"Greaves, R.I.N.; Nagington, J.; Kellaway, T.D.","year":"1963","unstructured":"Greaves, R. I. N., Nagington, J., and Kellaway, T. D. (1963). Fed. Proc., 22, 90.","journal-title":"Fed. Proc"},{"key":"ref_14","article-title":"Immobilization and Agglutination of Trichomonas foetus","volume-title":"\"Symposium, Council for International Organizations of Medical","author":"Kerr, W.R.","year":"1964","unstructured":"Kerr, W. R. (1964). \"Immobilization and Agglutination of Trichomonas foetus\", in \"Symposium, Council for International Organizations of Medical"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/0035-9203(61)90102-X","volume":"55","author":"Kott, H.; Adler, S.","year":"1961","unstructured":"Kott, H., and Adler, S. (1961). Trans. roy. Soc. trop. Med. Hyg., 55, 333.","journal-title":"Trans. roy. Soc. trop. Med. Hyg"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1111\/j.1550-7408.1961.tb01244.x","volume":"8","author":"Levine, N.D.; Andersen, F.L.","year":"1961","unstructured":"Levine, N. D., and Andersen, F. L. (1961). J. Protozool., 8, Suppl., p. 5.","journal-title":"J. Protozool"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1103\/PhysRevLett.9.347","volume":"9","author":"Losch, M.B.; Notzold, R.A.; Mehra, K.N.","year":"1962","unstructured":"Losch, M. B., Notzold, R. A., and Mehra, K. N. (1962). Ibid., 9, 347.","journal-title":"Ibid"},{"key":"ref_18","author":"Losch, M.B.; Notzold, R.A.; Mehra, K.N.; Marquardt, W.C.","year":"1955","unstructured":"Losch, M. B., Notzold, R. A., and Mehra, K. N. and Marquardt, W. C. (1955). Ibid., 2, 100."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1016\/0014-4894(58)90020-1","volume":"7","author":"Mizell, M.; Houlahan, D.A.","year":"1958","unstructured":"Mizell, M., and Houlahan, D. A. (1958). Exp. Parasit., 7, 236.","journal-title":"Exp. Parasit"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"226","DOI":"10.2307\/3276273","volume":"50","author":"Lindgren, R.D.; Ivey, M.H.","year":"1964","unstructured":"Lindgren, R. D., and Ivey, M. H. (1964). J. Parasit., 50, 226.","journal-title":"J. Parasit"},{"key":"ref_21","first-page":"1894","volume":"183","author":"Lovelock, J.E.; Bishop, M.W.H.","year":"1959","unstructured":"Lovelock, J. E., and Bishop, M. W. H. (1959). Nature (Lond.), 183, 1894.","journal-title":"Nature (Lond.)"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1016\/0014-4894(65)90028-7","volume":"16","author":"Lumsden, W.H.R.; Cunningham, M.P.; Webber, W.A.F.; van Hoeve, K.; Knight, R.H.; Simmons, V.","year":"1965","unstructured":"Lumsden, W. H. R., Cunningham, M. P., Webber, W. A. F., van Hoeve, K., Knight, R. H., and Simmons, V. (1965). Exp. Parasit., 16, 8.","journal-title":"Exp. Parasit"},{"key":"ref_23","first-page":"269","volume":"14","author":"Walker, P.J.","year":"1963","unstructured":"and Walker, P. J. (1963). Ibid., 14, 269.","journal-title":"Ibid"},{"key":"ref_24","first-page":"1032","volume":"205","author":"Walker, P.J.; Walker, P.J.; Walker, P.J.; Hardy, G.J.C.","year":"1965","unstructured":"and Walker, P. J. and Walker, P. J. and Walker, P. J. and Hardy, G. J. C. (1965). Nature (Lond.), 205, 1032.","journal-title":"Nature (Lond.)"},{"key":"ref_25","first-page":"336","article-title":"Antibody Response in Cattle to Infection with Trichomonas foetus\", in \"Immunity to Protozoa: A Symposium of the British Society for Immunology","author":"Robertson, M.","year":"1963","unstructured":"Robertson, M. (1963). \"Antibody Response in Cattle to Infection with Trichomonas foetus\", in \"Immunity to Protozoa: A Symposium of the British Society for Immunology\", ed. P. C. C. Garnham, A. E. Pierce, and I. Roitt, pp. 336-345. Blackwell Scientific Publications, Oxford."},{"key":"ref_26","article-title":"Biological Effects of Freezing and Supercooling","volume":"9","author":"Smith, A.U.","year":"1961","unstructured":"Smith, A. U. (1961). \"Biological Effects of Freezing and Supercooling\" (Monographs of the Physiological Society No. 9). Arnold, London.","journal-title":"(Monographs of the Physiological Society"},{"key":"ref_27","article-title":"Trichomonas Vaginalis and Trichomoniasis","author":"Trussell, R.E.","year":"1947","unstructured":"Trussell, R. E. (1947). \"Trichomonas Vaginalis and Trichomoniasis\". Blackwell, Oxford."},{"key":"ref_28","article-title":"Le milieu de culture de Feinberg et Whittington","volume-title":"pour le T. vaginalis a e examine afin de faciliter la manipulation de ses caracteristiques pour les besoins experimentaux. La concentration minima de son constituant plus fortement tamponne, le foie digere, pour la propagation du T. vaginalis a et definie. La reduction de ce constitu.ant jusqu'& ce niveau a ameliore le contr6le pH de ce milieu de culture","year":"1957","unstructured":"Le milieu de culture de Feinberg et Whittington (1957) pour le T. vaginalis a e examine afin de faciliter la manipulation de ses caracteristiques pour les besoins experimentaux. La concentration minima de son constituant plus fortement tamponne, le foie digere, pour la propagation du T. vaginalis a et definie. La reduction de ce constitu.ant jusqu'& ce niveau a ameliore le contr6le pH de ce milieu de culture."},{"key":"ref_29","article-title":"Le pH initial optimum de ce milieu de culture a et trouve dans la gamme","volume-title":"de 5,8 ia 6,4.","unstructured":"Le pH initial optimum de ce milieu de culture a et trouve dans la gamme de 5,8 ia 6,4."},{"key":"ref_30","article-title":"vaginalis ont et faits","author":"isolements du T, Six","unstructured":"Six isolements du T. vaginalis ont et faits."},{"key":"ref_31","article-title":"Des essais de plusieurs protecteurs geles et de deux taux de refrig6rations ont indique que la refrigeration lente jusqu'a -79\u00b0 C. en presence de dimethyle sulphoxide est une methode efficace pour la preparation et la preservation des \"stabilates\" du Trichomonas vaginalis, a en juger par les estimations de concentration des organismes et la proportion des organismes en mouvement","unstructured":"Des essais de plusieurs protecteurs geles et de deux taux de refrig6rations ont indique que la refrigeration lente jusqu'a -79\u00b0 C. en presence de dimethyle sulphoxide est une methode efficace pour la preparation et la preservation des \"stabilates\" du Trichomonas vaginalis, a en juger par les estimations de concentration des organismes et la proportion des organismes en mouvement."},{"key":"ref_32","article-title":"La n6cessite de mesurer la viabilite des suspensions des protozoaires parasitiques en general et du materiel preserve 'a basse temperature en particulier, independamment d'une e3timation purement visuelle, est accentuee. Une methode de titrage de l'infection causee par des cultures de suspensions de T. vaginalis est decrite, cette methode a n6cessite des series de dilutions des suspensions au 10eme et l'inoculation des groupes de cultures 'a plusieurs dilutions","unstructured":"La n6cessite de mesurer la viabilite des suspensions des protozoaires parasitiques en general et du materiel preserve 'a basse temperature en particulier, independamment d'une e3timation purement visuelle, est accentuee. Une methode de titrage de l'infection causee par des cultures de suspensions de T. vaginalis est decrite, cette methode a n6cessite des series de dilutions des suspensions au 10eme et l'inoculation des groupes de cultures 'a plusieurs dilutions."},{"key":"ref_33","article-title":"Le titrage de l'infection causee par les \"stabilates\" des T. vaginalis, preserves comme decrit, a indique que six ou moins d'organismes constituent generalement une dose suffisante pour causer l'infection des cultures","unstructured":"Le titrage de l'infection causee par les \"stabilates\" des T. vaginalis, preserves comme decrit, a indique que six ou moins d'organismes constituent generalement une dose suffisante pour causer l'infection des cultures."}],"container-title":["Sexually Transmitted Infections"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1136\/sti.42.3.145","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,25]],"date-time":"2020-09-25T23:31:28Z","timestamp":1601076688000},"score":1,"resource":{"primary":{"URL":"https:\/\/sti.bmj.com\/lookup\/doi\/10.1136\/sti.42.3.145"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1966,9,1]]},"references-count":33,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1966,9,1]]}},"URL":"https:\/\/doi.org\/10.1136\/sti.42.3.145","relation":{},"ISSN":["1368-4973"],"issn-type":[{"value":"1368-4973","type":"print"}],"subject":[],"published":{"date-parts":[[1966,9,1]]}}}