{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T12:31:11Z","timestamp":1783427471523,"version":"3.54.6"},"reference-count":38,"publisher":"BMJ","issue":"10","funder":[{"name":"National Institute for Health Research (NIHR) Manchester Biomedical Research Centre","award":["IS-BRC-1215-20007"],"award-info":[{"award-number":["IS-BRC-1215-20007"]}]},{"DOI":"10.13039\/501100000362","name":"Asthma and Lung UK","doi-asserted-by":"publisher","award":["AUK-AC-2018-01"],"award-info":[{"award-number":["AUK-AC-2018-01"]}],"id":[{"id":"10.13039\/501100000362","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Thorax"],"accepted":{"date-parts":[[2023,3,14]]},"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Inhaled mannitol provokes bronchoconstriction<jats:italic>via<\/jats:italic>mediators released during osmotic degranulation of inflammatory cells, and, hence represents a useful diagnostic test for asthma and model for acute attacks. We hypothesised that the mannitol challenge would trigger changes in exhaled volatile organic compounds (VOCs), generating both candidate biomarkers and novel insights into their origin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Participants with a clinical diagnosis of asthma, or undergoing investigation for suspected asthma, were recruited. Inhaled mannitol challenges were performed, followed by a sham challenge after 2 weeks in participants with bronchial hyper-responsiveness (BHR). VOCs were collected before and after challenges and analysed using gas chromatography\u2013mass spectrometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Forty-six patients (mean (SD) age 52 (16) years) completed a mannitol challenge, of which 16 (35%) were positive, and 15 of these completed a sham challenge. Quantities of 16 of 51 identified VOCs changed following mannitol challenge (p&lt;0.05), of which 11 contributed to a multivariate sparse partial least square discriminative analysis model, with a classification error rate of 13.8%. Five of these 16 VOCs also changed (p&lt;0.05) in quantity following the sham challenge, along with four further VOCs. In patients with BHR to mannitol distinct postchallenge VOC signatures were observed compared with post-sham challenge.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Inhalation of mannitol was associated with changes in breath VOCs, and in people with BHR resulted in a distinct exhaled breath profile when compared with a sham challenge. These differentially expressed VOCs are likely associated with acute airway inflammation and\/or bronchoconstriction and merit further investigation as potential biomarkers in asthma.<\/jats:p><\/jats:sec>","DOI":"10.1136\/thorax-2022-219708","type":"journal-article","created":{"date-parts":[[2023,7,26]],"date-time":"2023-07-26T18:17:20Z","timestamp":1690395440000},"page":"966-973","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":10,"title":["Changes in exhaled volatile organic compounds following indirect bronchial challenge in suspected asthma"],"prefix":"10.1136","volume":"78","clinical-trial-number":[{"clinical-trial-number":"nct03575663","registry":"10.18810\/clinical-trials-gov"}],"author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1185-9940","authenticated-orcid":false,"given":"Adam","family":"Peel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3324-969X","authenticated-orcid":false,"given":"Ran","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waqar","family":"Ahmed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iain","family":"White","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxim","family":"Wilkinson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoon K","family":"Loke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5514-4867","authenticated-orcid":false,"given":"Andrew M","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4524-1663","authenticated-orcid":false,"given":"Stephen J","family":"Fowler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"239","published-online":{"date-parts":[[2023,7,26]]},"reference":[{"key":"2024051320391412000_78.10.966.1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00165106"},{"key":"2024051320391412000_78.10.966.2","doi-asserted-by":"crossref","first-page":"918","DOI":"10.1016\/j.jaip.2017.05.001","article-title":"Asthma exacerbations: pathogenesis, prevention, and treatment","volume":"5","author":"Castillo","year":"2017","journal-title":"J Allergy Clin Immunol Pract"},{"key":"2024051320391412000_78.10.966.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.anai.2010.11.010"},{"key":"2024051320391412000_78.10.966.4","doi-asserted-by":"crossref","first-page":"1292","DOI":"10.1016\/j.jaci.2016.05.029","article-title":"Exercise-induced bronchoconstriction update-2016","volume":"138","author":"Weiler","year":"2016","journal-title":"J Allergy Clin Immunol"},{"key":"2024051320391412000_78.10.966.5","doi-asserted-by":"crossref","DOI":"10.1088\/1752-7163\/aa5a8b","article-title":"Can exhaled volatile organic compounds predict asthma exacerbations in children?","volume":"11","author":"van Vliet","year":"2017","journal-title":"J Breath Res"},{"key":"2024051320391412000_78.10.966.6","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1111\/cea.12965","article-title":"Exhaled breath profiles in the monitoring of loss of control and clinical recovery in asthma","volume":"47","author":"Brinkman","year":"2017","journal-title":"Clin Exp Allergy"},{"key":"2024051320391412000_78.10.966.7","doi-asserted-by":"publisher","DOI":"10.1136\/thx.2010.156695"},{"key":"2024051320391412000_78.10.966.8","doi-asserted-by":"crossref","first-page":"9127","DOI":"10.3390\/s101009127","article-title":"Electronic nose breathprints are independent of acute changes in airway caliber in asthma","volume":"10","author":"Lazar","year":"2010","journal-title":"Sensors (Basel)"},{"key":"2024051320391412000_78.10.966.9","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1016\/j.tibtech.2014.08.003","article-title":"Taking your breath away: metabolomics breathes life in to personalized medicine","volume":"32","author":"Rattray","year":"2014","journal-title":"Trends Biotechnol"},{"key":"2024051320391412000_78.10.966.10","unstructured":"BTS\/SIGN British guideline on the management of asthma. 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