{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T09:43:54Z","timestamp":1790502234385,"version":"4.1.0"},"reference-count":18,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2012,3,10]],"date-time":"2012-03-10T00:00:00Z","timestamp":1331337600000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:p>\n                    <jats:italic>Coxiella burnetii<\/jats:italic>\n                    infections are mostly subclinical in cattle, but can occasionally be associated with abortion. In the present study, 100 aborted fetuses or stillborn calves that were submitted for postmortem examination between September 2007 and March 2008 were examined for infection with\n                    <jats:italic>C burnetii<\/jats:italic>\n                    . Samples of both pooled fetal tissues and placental cotyledon were tested using a real\u2010time PCR assay. In addition, the sections of placental cotyledon were examined using immunohistochemistry (IHC). The IHC of four placentas was positive. The PCR results of the IHC\u2010positive placentas were high positive (HP); the PCR results of the organs of these four fetuses and calves varied from low positive (LP) to HP. The four IHC\u2010positive fetuses had a gestation length of seven to nine months. All four placentas had histological signs of inflammation, but only one of four placentas had gross pathological signs of inflammation possibly due to a concomitant infection with\n                    <jats:italic>Bacillus licheniformis<\/jats:italic>\n                    . Five other IHC\u2010negative placentas had (high) positive PCR results; the PCR results of the organs of these fetuses were LP or negative. The present study indicates that\n                    <jats:italic>C burnetii<\/jats:italic>\n                    infections are detected in a limited percentage of aborted fetuses and stillborn calves by IHC. To assess the importance of placentas with PCR\u2010positive and IHC\u2010negative test results, more research is needed.\n                  <\/jats:p>","DOI":"10.1136\/vr.100378","type":"journal-article","created":{"date-parts":[[2011,12,21]],"date-time":"2011-12-21T00:02:51Z","timestamp":1324425771000},"page":"260-260","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":32,"title":["Prevalence of\n                    <i>Coxiella burnetii<\/i>\n                    infections in aborted fetuses and stillborn calves"],"prefix":"10.1002","volume":"170","author":[{"given":"J.","family":"Muskens","sequence":"first","affiliation":[{"name":"Ruminant Health Department Animal Health Service PO BOX 9 Deventer 7400 AA Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Wouda","sequence":"additional","affiliation":[{"name":"Ruminant Health Department Animal Health Service PO BOX 9 Deventer 7400 AA Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"von Bannisseht\u2010Wijsmuller","sequence":"additional","affiliation":[{"name":"Ruminant Health Department Animal Health Service PO BOX 9 Deventer 7400 AA Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"van Maanen","sequence":"additional","affiliation":[{"name":"Ruminant Health Department Animal Health Service PO BOX 9 Deventer 7400 AA Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,3,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2005010"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063870001200505"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2006038"},{"key":"e_1_2_6_5_1","first-page":"131","article-title":"Bacillus licheniformis associated with abortions in cows","volume":"66","author":"Hommez J.","year":"1997","journal-title":"Vlaams Diergeneeskundig Tijdschrift"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0463.2007.apm_591.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.c4040"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.11.4234-4237.1998"},{"key":"e_1_2_6_9_1","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/j.vetpar.2004.08.016","article-title":"An interlaboratory comparison of immunohistochemistry and PCR methods for detection of Neospora caninum in bovine fetal tissues.","volume":"126","author":"Van Maanen C.","year":"2004","journal-title":"Veterinary Parasitology"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2004.01.006"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.4.518"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9975(08)80254-X"},{"key":"e_1_2_6_13_1","first-page":"368","article-title":"[Stillbirth\/perinatal weak calf syndrome in dairy heifers: results of a pilot study].","volume":"129","author":"Muskens J.","year":"2004","journal-title":"Tijdschrift Voor Diergeneeskunde"},{"key":"e_1_2_6_14_1","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1136\/vr.d4693","article-title":"Prevalence of Coxiella burnetii in livestock abortion material using PCR.","volume":"169","author":"Pritchard G. 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