{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:41:24Z","timestamp":1791294084411,"version":"4.1.0"},"reference-count":14,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,7,21]],"date-time":"2012-07-21T00:00:00Z","timestamp":1342828800000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:p>\n                    This is the first report of detection of\n                    <jats:italic>Candidatus<\/jats:italic>\n                    Mycoplasma haemolamae in alpacas in England. The primary case occurred in a three year\u2010old male alpaca in the south\u2010east of England which presented with a history of progressive weight loss, lethargy, swelling of the scrotum and pale mucous membranes. Blood smear examination revealed a moderate, regenerative anaemia, with numerous small basophilic coccoid structures consistent with\n                    <jats:italic>Candidatus<\/jats:italic>\n                    M haemolamae. To confirm the presence of\n                    <jats:italic>Candidatus<\/jats:italic>\n                    M haemolamae, a portion of the 16S rDNA gene was amplified and analysed by denaturing gradient gel electrophoresis (DGGE). 16S rDNA gene sequencing showed a 99.8 per cent homology with\n                    <jats:italic>Candidatus<\/jats:italic>\n                    M haemolamae sequences deposited in GenBank. Subsequently, a cross\u2010sectional study was carried out to investigate the presence of Candidatus M haemolamae infection in the alpaca herd from which the primary case was detected (n=131). Blood smear examinations and PCR with DGGE were used and compared with a species\u2010specific PCR. The prevalence of infection when PCR positive results were combined was 29 per cent. A substantial agreement between the PCR\/DGGE and the species\u2010specific PCR was found (\u03ba=0.86). A significant association was also found between age and infection (P=0.04) while no significant association was found with sex or origin.\n                  <\/jats:p>","DOI":"10.1136\/vr.100611","type":"journal-article","created":{"date-parts":[[2012,7,10]],"date-time":"2012-07-10T20:55:48Z","timestamp":1341953748000},"page":"71-71","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":16,"title":["First detection of \u2018\n                    <i>Candidatus<\/i>\n                    Mycoplasma haemolamae\u2019 infection in alpacas in England"],"prefix":"10.1002","volume":"171","author":[{"given":"P.","family":"Crosse","sequence":"first","affiliation":[{"name":"Department of Pathology and Infectious Diseases Department of Veterinary Clinical Sciences Royal Veterinary College Hawkshead Lane, North Mymms Hertfordshire AL9 7TA UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Ayling","sequence":"additional","affiliation":[{"name":"Mycoplasma Group Department of Bacteriology Veterinary Laboratories Agency (Weybridge) Surrey KT15 3NB UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Whitehead","sequence":"additional","affiliation":[{"name":"Department of Pathology and Infectious Diseases Department of Veterinary Clinical Sciences Royal Veterinary College Hawkshead Lane, North Mymms Hertfordshire AL9 7TA UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Szladovits","sequence":"additional","affiliation":[{"name":"Department of Pathology and Infectious Diseases Department of Veterinary Clinical Sciences Royal Veterinary College Hawkshead Lane, North Mymms Hertfordshire AL9 7TA UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"English","sequence":"additional","affiliation":[{"name":"Department of Pathology and Infectious Diseases Department of Veterinary Clinical Sciences Royal Veterinary College Hawkshead Lane, North Mymms Hertfordshire AL9 7TA UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Bradley","sequence":"additional","affiliation":[{"name":"Mycoplasma Group Department of Bacteriology Veterinary Laboratories Agency (Weybridge) Surrey KT15 3NB UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Solano\u2010Gallego","sequence":"additional","affiliation":[{"name":"Department of Pathology and Infectious Diseases Department of Veterinary Clinical Sciences Royal Veterinary College Hawkshead Lane, North Mymms Hertfordshire AL9 7TA UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,7,21]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"229","article-title":"Mycoplasma haemolamae infection in a 4\u2010day\u2010old cria: support for in utero transmission by use of a polymerase chain reaction assay.","volume":"47","author":"Almy F. 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