{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T11:39:46Z","timestamp":1761565186080,"version":"build-2065373602"},"reference-count":37,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2013,4,20]],"date-time":"2013-04-20T00:00:00Z","timestamp":1366416000000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:p>\n                    <jats:italic>Mannheimia haemolytica<\/jats:italic>\n                    is the aetiological agent of pneumonic pasteurellosis in small ruminants. The primary virulence factor of the bacterium is a leukotoxin (LktA), which induces apoptosis in susceptible cells via mitochondrial targeting. It has been previously shown that certain\n                    <jats:italic>lktA<\/jats:italic>\n                    alleles are associated either with cattle or sheep. The objective of the present study was to investigate\n                    <jats:italic>lktA<\/jats:italic>\n                    sequence variation among ovine and caprine\n                    <jats:italic>M haemolytica<\/jats:italic>\n                    strains isolated from pneumonic lungs, revealing any potential adaptation for the caprine host, for which there is no available data. Furthermore, we investigated amino acid variation in the N\u2010terminal part of the sequences and its effect on targeting mitochondria. Data analysis showed that the prevalent caprine genotype differed at a single non\u2010synonymous site from a previously described uncommon bovine allele, whereas the ovine sequences represented new, distinct alleles. N\u2010terminal sequence differences did not affect the mitochondrial targeting ability of the isolates; interestingly enough in one case, mitochondrial matrix targeting was indicated rather than membrane association, suggesting an alternative LktA trafficking pattern.\n                  <\/jats:p>","DOI":"10.1136\/vr.101014","type":"journal-article","created":{"date-parts":[[2013,2,8]],"date-time":"2013-02-08T22:53:38Z","timestamp":1360364018000},"page":"424-424","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":2,"title":["Sequence diversity of the leukotoxin (\n                    <i>lktA<\/i>\n                    ) gene in caprine and ovine strains of\n                    <i>Mannheimia haemolytica<\/i>"],"prefix":"10.1002","volume":"172","author":[{"given":"C.","family":"Vougidou","sequence":"first","affiliation":[{"name":"Institute of Infectious and Parasitic Diseases of Thessaloniki Ministry of Rural Development and Food Thessaloniki 54627 Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Sandalakis","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Bacteriology, Parasitology, Zoonoses, and Geographical Medicine WHO Collaborating Center University of Cretez Crete 71110 Greece"},{"name":"Hellenic Center for Disease Control &amp; Prevention Regional Laboratory of Public Health (Crete) Crete 71110 Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Psaroulaki","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Bacteriology, Parasitology, Zoonoses, and Geographical Medicine WHO Collaborating Center University of Cretez Crete 71110 Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Petridou","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology and Infectious Diseases Veterinary School Aristotle University of Thessaloniki Thessaloniki 54124 Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Ekateriniadou","sequence":"additional","affiliation":[{"name":"Veterinary Research Institute of Thessaloniki NAGREF Campus of Thermi Thessaloniki 57001 Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,4,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2008.01.001"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2004.12.007"},{"key":"e_1_2_7_4_1","first-page":"233","article-title":"Pasteurella haemolytica complicated respiratory infections in sheep and goats","volume":"29","author":"Brogden K. 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