{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T15:35:17Z","timestamp":1770219317542,"version":"3.49.0"},"reference-count":15,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2012,11,17]],"date-time":"2012-11-17T00:00:00Z","timestamp":1353110400000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:p>\n                    A commercial coproantigen ELISA test for fasciolosis, based on the use of MM3\u00a0monoclonal antibody for antigen capture, was investigated for possible cross\u2010reactivity with\n                    <jats:bold>\n                      <jats:italic>Paramphistomum cervi<\/jats:italic>\n                    <\/jats:bold>\n                    , a trematode that commonly infects cattle and sheep grazing in fluke\u2010infested pasture in Ireland. Histological sections of adult and immature\n                    <jats:bold>\n                      <jats:italic>Fasciola hepatica<\/jats:italic>\n                    <\/jats:bold>\n                    and\n                    <jats:bold>\n                      <jats:italic>P cervi<\/jats:italic>\n                    <\/jats:bold>\n                    were incubated with MM3\u00a0monoclonal antibody, and its binding to tissue\u2010localised coproantigen was subsequently visualised by immunocytochemistry. In a related study, the soluble antigenic fractions derived from homogenates of\n                    <jats:bold>\n                      <jats:italic>P cervi<\/jats:italic>\n                    <\/jats:bold>\n                    adults and\n                    <jats:bold>\n                      <jats:italic>Taenia hydatigena<\/jats:italic>\n                    <\/jats:bold>\n                    metacestodes were tested for cross\u2010reactivity with MM3\u00a0monoclonal antibody in an antigen\u2010capture ELISA, using known\n                    <jats:bold>\n                      <jats:italic>F hepatica<\/jats:italic>\n                    <\/jats:bold>\n                    \u2010positive and\n                    <jats:bold>\n                      <jats:italic>F hepatica<\/jats:italic>\n                    <\/jats:bold>\n                    \u2010negative ovine faecal samples as natural controls. It was found that, while intense immunocytochemical labelling was located over the gastrodermis and gut contents of adult and immature\n                    <jats:bold>\n                      <jats:italic>F hepatica<\/jats:italic>\n                    <\/jats:bold>\n                    , sections of adult and immature\n                    <jats:bold>\n                      <jats:italic>P cervi<\/jats:italic>\n                    <\/jats:bold>\n                    were unlabelled. In the ELISA tests, the soluble fractions of\n                    <jats:bold>\n                      <jats:italic>F hepatica<\/jats:italic>\n                    <\/jats:bold>\n                    reacted strongly with MM3\u00a0monoclonal antibody, but those of\n                    <jats:bold>\n                      <jats:italic>P cervi<\/jats:italic>\n                    <\/jats:bold>\n                    and\n                    <jats:bold>\n                      <jats:italic>T hydatigena<\/jats:italic>\n                    <\/jats:bold>\n                    gave negative results. These findings support the specificity of the coproantigen ELISA test for fasciolosis in areas where paramphistomosis and cysticercosis are liable to occur singly or as coinfections with\n                    <jats:bold>\n                      <jats:italic>F hepatica<\/jats:italic>\n                    <\/jats:bold>\n                    .\n                  <\/jats:p>","DOI":"10.1136\/vr.101041","type":"journal-article","created":{"date-parts":[[2012,10,31]],"date-time":"2012-10-31T10:30:11Z","timestamp":1351679411000},"page":"502-502","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":23,"title":["Specificity of a coproantigen ELISA test for fasciolosis: lack of cross\u2010reactivity with\n                    <i>Paramphistomum cervi<\/i>\n                    and\n                    <i>Taenia hydatigena<\/i>"],"prefix":"10.1002","volume":"171","author":[{"given":"P\u2010E.","family":"Kajugu","sequence":"first","affiliation":[{"name":"Disease Surveillance and Investigation Branch Veterinary Sciences Division Agri\u2010Food and Biosciences Institute Belfast BT4 3SD UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. E. B.","family":"Hanna","sequence":"additional","affiliation":[{"name":"Disease Surveillance and Investigation Branch Veterinary Sciences Division Agri\u2010Food and Biosciences Institute Belfast BT4 3SD UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. W.","family":"Edgar","sequence":"additional","affiliation":[{"name":"Disease Surveillance and Investigation Branch Veterinary Sciences Division Agri\u2010Food and Biosciences Institute Belfast BT4 3SD UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. I.","family":"Forster","sequence":"additional","affiliation":[{"name":"Disease Surveillance and Investigation Branch Veterinary Sciences Division Agri\u2010Food and Biosciences Institute Belfast BT4 3SD UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. E.","family":"Malone","sequence":"additional","affiliation":[{"name":"Disease Surveillance and Investigation Branch Veterinary Sciences Division Agri\u2010Food and Biosciences Institute Belfast BT4 3SD UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. P.","family":"Brennan","sequence":"additional","affiliation":[{"name":"Parasite Therapeutics Research Group School of Biological Sciences Queen's University Belfast BT9 7BL UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Fairweather","sequence":"additional","affiliation":[{"name":"Parasite Therapeutics Research Group School of Biological Sciences Queen's University Belfast BT9 7BL UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,11,17]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"42","article-title":"A joint AFBI\/DAFF Veterinary Laboratories publication","author":"ANON","year":"2010","journal-title":"All\u2010island animal disease surveillance report 2010"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M308831200"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molbiopara.2009.04.005"},{"key":"e_1_2_6_5_2","first-page":"313","article-title":"Paramphistomum \u2013 a brief review","volume":"63","author":"De Waal T.","year":"2010","journal-title":"Irish Veterinary Journal"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0020-7519(87)90166-4"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.10.057"},{"key":"e_1_2_6_8_2","doi-asserted-by":"crossref","unstructured":"FlanaganA. M.EdgarH. W. J.ForsterF.GordonA.HannaR. E. B.McCoyM.BrennanG. P.FairweatherI.(2011b)Standardisation of a coproantigen reduction test (CRT) protocol for the diagnosis of resistance to triclabendazole inFasciola hepatica. Veterinary Parasitology176 34\u201342","DOI":"10.1016\/j.vetpar.2010.10.037"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(75)90019-3"},{"key":"e_1_2_6_10_2","volume-title":"Association of Veterinary Teachers and Research Workers (Irish Branch) 45th Annual Scientific Meeting","author":"Kajugu P.\u2010E.","year":"2011"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.12.6921-6932.2003"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2009.02.004"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1645\/GE-192R"},{"key":"e_1_2_6_14_2","first-page":"399","article-title":"Triclabendazole\u2010resistant liver fluke in Scottish sheep","volume":"143","author":"Mitchell G. B. 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