{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T11:00:32Z","timestamp":1761562832435,"version":"build-2065373602"},"reference-count":51,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2014,4,26]],"date-time":"2014-04-26T00:00:00Z","timestamp":1398470400000},"content-version":"vor","delay-in-days":25,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:p>Porcine circovirus type 2 (PCV2) causes some of the most significant economic losses in pig production. Several multisystemic syndromes have been attributed to PCV2\u00a0infection, which are known as PCV2\u2010associated diseases (PCVDs). This study investigated the origin and evolution of PCV2 sequences in domestic pigs and wild boars affected by PCVDs in Croatia. Viral sequences were recovered from three wild boars diagnosed with PCV2\u2010systemic disease (PCV2\u2010SD), 63 fetuses positive for PCV2 DNA as determined by PCR, 14 domestic pigs affected with PCV2\u2010SD (displaying severe interstitial nephritis) and five domestic pigs with proliferative and necrotising pneumonia. Seventeen complete PCV2 genomes were recovered. Phylogenetic and evolutionary analyses based on median\u2010joining phylogenetic networks, amino acid alignments and principal coordinate analysis were performed using complete genomes, as well as complete and partial ORF sequences for ORF1 and ORF2. Two of the 17\u00a0PCV2 sequences belonged to PCV2a, 14 to PCV2b and one was unclustered. PCV2b was the predominant genotype in Croatia and has been linked to international trade as a route of introduction. Correlation between particular viral strains with PCVDs is lacking.<\/jats:p>","DOI":"10.1136\/vr.102064","type":"journal-article","created":{"date-parts":[[2014,3,4]],"date-time":"2014-03-04T00:53:36Z","timestamp":1393894416000},"page":"431-431","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":4,"title":["Origin of porcine circovirus type 2 (PCV2) from swine affected by PCV2\u2010associated diseases in Croatia"],"prefix":"10.1002","volume":"174","author":[{"given":"D.","family":"Novosel","sequence":"first","affiliation":[{"name":"Department of Pathology Croatian Veterinary Institute Zagreb 10000 Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Tuboly","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases Faculty of Veterinary Science Szent Istv\u00e1n University Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Csagola","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases Faculty of Veterinary Science Szent Istv\u00e1n University Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Lorincz","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases Faculty of Veterinary Science Szent Istv\u00e1n University Budapest Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Cubric\u2010Curik","sequence":"additional","affiliation":[{"name":"Department of Animal Science Faculty of Agriculture University of Zagreb Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Jungic","sequence":"additional","affiliation":[{"name":"Virology Department Croatian Veterinary Institute Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Curik","sequence":"additional","affiliation":[{"name":"Department of Animal Science Faculty of Agriculture University of Zagreb Zagreb Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Segal\u00e9s","sequence":"additional","affiliation":[{"name":"Centre de Recerca en Sanitat Animal (CReSA) UAB\u2010IRTA Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Cortey","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Virologie Mol\u00e9culaire Emergence et Co\u2010\u00e9volution Virale Marseille France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Z.","family":"Lipej","sequence":"additional","affiliation":[{"name":"Department of Pathology Croatian Veterinary Institute Zagreb 10000 Croatia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,4,26]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_8_2_1","DOI":"10.1016\/j.virusres.2011.09.013"},{"key":"e_1_2_8_3_1","first-page":"17","article-title":"Some biological and physico\u2010chemical properties of porcine circovirus","volume":"41","author":"Allan G. 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