{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T18:31:12Z","timestamp":1784140272908,"version":"3.55.0"},"reference-count":35,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2020,4,18]],"date-time":"2020-04-18T00:00:00Z","timestamp":1587168000000},"content-version":"vor","delay-in-days":17,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,2,17]],"date-time":"2020-02-17T00:00:00Z","timestamp":1581897600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/N002121\/1"],"award-info":[{"award-number":["BB\/N002121\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/N002121\/1"],"award-info":[{"award-number":["BB\/N002121\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Non\u2010healing bovine foot lesions, including non\u2010healing white line disease, non\u2010healing sole ulcer and toe necrosis, are an increasingly important cause of chronic lameness that are poorly responsive to treatment. Recent studies have demonstrated a high\u2010level association between these non\u2010healing lesions and the\n                      <jats:italic>Treponema<\/jats:italic>\n                      phylogroups implicated in bovine digital dermatitis (BDD). However, a polymicrobial aetiology involving other gram\u2010stain\u2010negative anaerobes is suspected.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A PCR\u2010based bacteriological survey of uncomplicated BDD lesions (n=10) and non\u2010healing bovine foot lesions (n=10) targeting\n                      <jats:italic>Fusobacterium necrophorum<\/jats:italic>\n                      ,\n                      <jats:italic>Porphyromonas endodontalis, Dichelobacter nodosus<\/jats:italic>\n                      and\n                      <jats:italic>Treponema pallidum\/T. paraluiscuniculi<\/jats:italic>\n                      was performed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:italic>P. endodontalis<\/jats:italic>\n                      DNA was detected in 80.0% of the non\u2010healing lesion biopsies (p=&lt;0.001) but was entirely absent from uncomplicated BDD lesion biopsies. When compared to the BDD lesions,\n                      <jats:italic>F. necrophorum<\/jats:italic>\n                      was detected at a higher frequency in the non\u2010healing lesions (33.3% vs 70.0%, respectively), whereas\n                      <jats:italic>D. nodosus<\/jats:italic>\n                      was detected at a lower frequency (55.5% vs 20.0%, respectively). Conversely,\n                      <jats:italic>T. pallidum\/T. paraluiscuniculi<\/jats:italic>\n                      DNA was not detected in either lesion type.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The data from this pilot study suggest that\n                      <jats:italic>P. endodontalis<\/jats:italic>\n                      and\n                      <jats:italic>F. necrophorum<\/jats:italic>\n                      should be further investigated as potential aetiological agents of non\u2010healing bovine foot lesions. A failure to detect syphilis treponemes in either lesion type is reassuring given the potential public health implications such an infection would present.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/vr.105628","type":"journal-article","created":{"date-parts":[[2020,2,17]],"date-time":"2020-02-17T17:25:20Z","timestamp":1581960320000},"page":"450-450","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":18,"title":["Surveying bovine digital dermatitis and non\u2010healing bovine foot lesions for the presence of\n                    <i>Fusobacterium necrophorum, Porphyromonas endodontalis<\/i>\n                    and\n                    <i>Treponema pallidum<\/i>"],"prefix":"10.1002","volume":"186","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4100-8597","authenticated-orcid":false,"given":"Gareth James","family":"Staton","sequence":"first","affiliation":[{"name":"Institute of Infection 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