{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T19:11:10Z","timestamp":1790104270101,"version":"4.0.1"},"reference-count":33,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2020,6,25]],"date-time":"2020-06-25T00:00:00Z","timestamp":1593043200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100011004","name":"Minist\u00e8re de l'Agriculture et de l'Alimentation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100011004","id-type":"DOI","asserted-by":"publisher"}]},{"name":"C\u00f4tes d\u2019Armor departmental council"},{"name":"French Ministry of Agriculture"},{"name":"French Agency for Food, Environmental and Occupational Health Safety"},{"name":"ANIBOTNET project"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Persistence of\n                      <jats:italic>Clostridium botulinum<\/jats:italic>\n                      in the environment is well known. Getting rid of it after animal botulism outbreaks is so tricky, especially as far as manure concerns. This study aimed at 1. describing manure management on 10 poultry farms affected by botulism and 2. assessing the persistence of\n                      <jats:italic>C botulinum<\/jats:italic>\n                      in poultry manure after the outbreak.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Each farm was visited twice at two different manure storage times (two weeks after manure removal and two months later). Fifteen samples of manure were collected on each visit and\n                      <jats:italic>C botulinum<\/jats:italic>\n                      was detected using real\u2010time PCR.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Management of manure varied among poultry farms (classical storage, addition of quicklime, bacterial flora or incineration).\n                      <jats:italic>C botulinum<\/jats:italic>\n                      was detected in the manure of all 10 farms, 56.5per cent of samples being positive.\n                      <jats:italic>C botulinum<\/jats:italic>\n                      was detected significantly more frequently at the second visit (65.8per cent vs 49.7per cent, P&lt;0.01) and on the surface of the pile (63.1per cent vs 50per cent, P=0.025).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      This study shows the persistence of\n                      <jats:italic>C botulinum<\/jats:italic>\n                      in poultry manure over time after a botulism outbreak and highlights manure management as a key health issue in preventing spore dissemination in the environment and recurrence of the disease.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/vr.105898","type":"journal-article","created":{"date-parts":[[2020,6,25]],"date-time":"2020-06-25T17:15:37Z","timestamp":1593105337000},"page":"233-233","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":10,"title":["Manure contamination with\n                    <i>Clostridium botulinum<\/i>\n                    after avian botulism outbreaks: management and potential risk of dissemination"],"prefix":"10.1002","volume":"187","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6792-4513","authenticated-orcid":false,"given":"Rozenn","family":"Souillard","sequence":"first","affiliation":[{"name":"Epidemiology, Health and Welfare Unit ANSES, French Agency for Food, Environmental and Occupational Health Safety Ploufragan France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"LE Marechal","sequence":"additional","affiliation":[{"name":"Hygiene and Quality of Poultry and Pig Products Unit ANSES, French Agency for Food, Environmental and Occupational Health Safety Ploufragan France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Loic","family":"Balaine","sequence":"additional","affiliation":[{"name":"Epidemiology, Health and Welfare Unit ANSES, French Agency for Food, Environmental and Occupational Health Safety Ploufragan France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Rouxel","sequence":"additional","affiliation":[{"name":"Hygiene and Quality of Poultry and Pig Products Unit ANSES, French Agency for Food, Environmental and Occupational Health Safety Ploufragan France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Typhaine","family":"Poezevara","sequence":"additional","affiliation":[{"name":"Hygiene and Quality of Poultry and Pig Products Unit ANSES, French Agency for Food, Environmental and Occupational Health Safety Ploufragan France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentine","family":"Ballan","sequence":"additional","affiliation":[{"name":"Hygiene and Quality of Poultry and Pig Products Unit ANSES, French Agency for Food, Environmental and Occupational Health Safety Ploufragan France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianne","family":"Chemaly","sequence":"additional","affiliation":[{"name":"Hygiene and Quality of Poultry and Pig Products Unit ANSES, French Agency for Food, Environmental and Occupational Health Safety Ploufragan France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"LE Bouquin","sequence":"additional","affiliation":[{"name":"Epidemiology, Health and Welfare Unit ANSES, French Agency for Food, Environmental and Occupational Health Safety Ploufragan France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,6,25]]},"reference":[{"key":"e_1_2_11_2_2","first-page":"953","volume-title":"In diseases of poultry 13th edition","author":"Skarin 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