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Information regarding EP presence in Ireland is currently limited. This retrospective surveillance study describes a serological and molecular analysis of blood samples submitted to the Irish Equine Centre for EP testing between January 2013 and April 2016.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Following serological testing, seropositive samples were screened using a PCR targeting the 18S ribosomal RNA gene. Amplicon sequences were bioinformatically analysed to identify the parasite species and to assess genetic diversity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      From 2099 screened equine blood samples, 2.5 per cent and 1 per cent were seropositive for\n                      <jats:italic>Theileria equi<\/jats:italic>\n                      and\n                      <jats:italic>Babesia caballi<\/jats:italic>\n                      , respectively.\n                      <jats:italic>T equi<\/jats:italic>\n                      DNA was detected in 9 per cent of the seropositive samples while\n                      <jats:italic>B caballi<\/jats:italic>\n                      DNA was not detected in any sample. The\n                      <jats:italic>T equi<\/jats:italic>\n                      DNA sequences displayed no genetic diversity at this locus, in contrast to samples from the UK and from endemic areas.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Detection of EP\u2010seropositive and parasitaemic horses in Ireland indicates a clear and present health risk to the equine population. It is recommended that owners adopt appropriate biosecurity measures and that clinicians are mindful of this disease as a differential diagnosis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1136\/vr.105937","type":"journal-article","created":{"date-parts":[[2020,9,4]],"date-time":"2020-09-04T17:21:45Z","timestamp":1599240105000},"update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":11,"title":["Investigating the presence of equine piroplasmosis in Ireland"],"prefix":"10.1002","volume":"187","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6905-3915","authenticated-orcid":false,"given":"Robert M","family":"Coultous","sequence":"first","affiliation":[{"name":"University of Glasgow Institute of Biodiversity, Animal Health and Comparative Medicine Glasgow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Desmond P","family":"Leadon","sequence":"additional","affiliation":[{"name":"Irish Equine Centre Naas Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian R","family":"Shiels","sequence":"additional","affiliation":[{"name":"University of Glasgow Institute of Biodiversity, Animal Health and Comparative Medicine Glasgow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Sutton","sequence":"additional","affiliation":[{"name":"University of Glasgow School of Veterinary Medicine Glasgow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Weir","sequence":"additional","affiliation":[{"name":"University of Glasgow School of Veterinary Medicine Glasgow UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,9,4]]},"reference":[{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2013.03.189"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010ento\u2010010814\u2010021110"},{"key":"e_1_2_11_4_2","unstructured":"GerstenbergC AllenWR PhippsLP.The mechanical transmission of Babesia equi infection in a British herd of horses. 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