{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T09:11:13Z","timestamp":1790673073904,"version":"4.1.0"},"reference-count":15,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[1996,9,7]],"date-time":"1996-09-07T00:00:00Z","timestamp":842054400000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[1996,9]]},"abstract":"<jats:p>\n                    Three groups of three pigs were vaccinated either with vaccine VAC\u2010SLY, containing purified suilysin derived from\n                    <jats:italic>Streptococcus suis<\/jats:italic>\n                    strain P1\/7 (serotype 2), or with vaccine VAC\u2010SCF, containing most of the other extracellular antigens produced by strain P1\/7 (but essentially free from suilysin), or with a placebo vaccine. The pigs were vaccinated twice at four weeks and six weeks of age and were challenged intravenously with\n                    <jats:italic>S suis<\/jats:italic>\n                    strain P1\/7 at eight weeks of age. On the day of challenge, only the VAC\u2010SLY vaccinated pigs showed an increase in haemolysin neutralisation titre. After challenge the placebo vaccinated pigs developed severe clinical signs characterised by lameness involving several joints, a depressed appearance, high temperatures and\/or neurological signs. The VAC\u2010SCF vaccinated pigs showed the same clinical signs but less severely. The VAC\u2010SLY vaccinated pigs were the least affected and showed only mild signs which subsided more quickly than those of the other groups. A post mortem investigation and histology of brain tissue samples confirmed the clinical findings; fibrinous arthritis was less severe and less frequently observed in the VAC\u2010SLY vaccinated pigs than in the VAC\u2010SCF or placebo vaccinated pigs, and none of the VAC\u2010SLY vaccinated pigs had meningitis whereas two of the VAC\u2010SCF and two of the placebo vaccinated pigs did so. All the samples of brain, lung and tarsus taken from the VAC\u2010SLY vaccinated pigs were sterile whereas\n                    <jats:italic>S suis<\/jats:italic>\n                    was reisolated from most of these tissues from the other groups.\n                  <\/jats:p>","DOI":"10.1136\/vr.139.10.225","type":"journal-article","created":{"date-parts":[[2010,10,4]],"date-time":"2010-10-04T22:39:48Z","timestamp":1286231988000},"page":"225-228","source":"Crossref","is-referenced-by-count":78,"title":["Protection of experimentally infected pigs by suilysin, the thiol\u2010activated haemolysin of\n                    <i>Streptococcus suis<\/i>"],"prefix":"10.1002","volume":"139","author":[{"given":"A. A. C.","family":"Jacobs","sequence":"first","affiliation":[{"name":"Bacteriological Research Department Intervet International BV PO Box 31 5830 AA Boxmeer The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. J. G.","family":"van den Berg","sequence":"additional","affiliation":[{"name":"Bacteriological Research Department Intervet International BV PO Box 31 5830 AA Boxmeer The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. L. W.","family":"Loeffen","sequence":"additional","affiliation":[{"name":"Bacteriological Research Department Intervet International BV PO Box 31 5830 AA Boxmeer The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1996,9,7]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0934-8840(11)80372-5"},{"key":"e_1_2_5_3_1","doi-asserted-by":"crossref","first-page":"1256","DOI":"10.1128\/jcm.32.5.1256-1260.1994","volume":"32","author":"FEDER I.","year":"1994","journal-title":"Journal of Clinical Microbiology"},{"key":"e_1_2_5_4_1","first-page":"170","volume":"34","author":"FLORES J. L. M.","year":"1993","journal-title":"Canadian Veterinary Journal"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(92)90094-A"},{"key":"e_1_2_5_6_1","first-page":"208","volume-title":"Proceedings of the 13th International Pig Veterinary Society Congress","author":"GOTTSCHALK M.","year":"1994"},{"key":"e_1_2_5_7_1","doi-asserted-by":"crossref","first-page":"2895","DOI":"10.1128\/jcm.31.11.2895-2900.1993","volume":"31","author":"HAMPSON D. J.","year":"1993","journal-title":"Journal of Clinical Microbiology"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063879000200324"},{"key":"e_1_2_5_9_1","first-page":"27","volume":"33","author":"HIGGINS R.","year":"1992","journal-title":"Canadian Veterinary Journal"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0034-5288(18)31503-0"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9975(08)80138-7"},{"key":"e_1_2_5_12_1","doi-asserted-by":"crossref","first-page":"1742","DOI":"10.1128\/iai.62.5.1742-1748.1994","volume":"62","author":"JACOBS A. A. C.","year":"1994","journal-title":"Infection and Immunity"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.137.12.295"},{"key":"e_1_2_5_14_1","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/0378-1135(90)90112-9","volume":"22","author":"KEBEBE M.","year":"1990","journal-title":"Veterinary Microbiology"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.59.9.3156-3162.1991"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.60.2.550-556.1992"}],"container-title":["Veterinary Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1136%2Fvr.139.10.225","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bvajournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1136\/vr.139.10.225","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T10:44:56Z","timestamp":1761475496000},"score":1,"resource":{"primary":{"URL":"https:\/\/bvajournals.onlinelibrary.wiley.com\/doi\/10.1136\/vr.139.10.225"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,9]]},"references-count":15,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1996,9]]}},"alternative-id":["10.1136\/vr.139.10.225"],"URL":"https:\/\/doi.org\/10.1136\/vr.139.10.225","archive":["Portico"],"relation":{},"ISSN":["0042-4900","2042-7670"],"issn-type":[{"value":"0042-4900","type":"print"},{"value":"2042-7670","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,9]]}}}