{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T03:29:25Z","timestamp":1789615765359,"version":"build-2803163510"},"reference-count":18,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[1998,5,9]],"date-time":"1998-05-09T00:00:00Z","timestamp":894672000000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[1998,5]]},"abstract":"<jats:p>\n                    A polymerase chain reaction (PCR) assay was used to detect\n                    <jats:italic>Brucella<\/jats:italic>\n                    species in the uterus, udder, spleen, lymph nodes, kidney and liver of three cows which had been naturally infected in an outbreak of brucellosis, and the results were compared with the results of bacteriological investigations. All 18 samples reacted positively in the PCR, but five samples had weak bands after the electrophoretic separation of PCR mixtures. No\n                    <jats:italic>Brucella<\/jats:italic>\n                    strains could be detected in these five samples by bacterial cultivation, but all the other samples gave positive results. A pre\u2010enrichment procedure was necessary for the PCR. A PCR with DNA from eight\n                    <jats:italic>Yersinia<\/jats:italic>\n                    strains gave no amplification product.\n                  <\/jats:p>","DOI":"10.1136\/vr.142.19.512","type":"journal-article","created":{"date-parts":[[2010,10,4]],"date-time":"2010-10-04T23:03:59Z","timestamp":1286233439000},"page":"512-514","source":"Crossref","is-referenced-by-count":14,"title":["Detection of\n                    <i>Brucella<\/i>\n                    species in organs of naturally infected cattle by polymerase chain reaction"],"prefix":"10.1002","volume":"142","author":[{"given":"P.","family":"Gallien","sequence":"first","affiliation":[{"name":"Federal Institute for Health Protection of Consumers and Veterinary Medicine Jahnstrasse 8 06846 Dessau Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Dorn","sequence":"additional","affiliation":[{"name":"Federal Institute for Health Protection of Consumers and Veterinary Medicine Diedersdorfer Weg 1 12277 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Alban","sequence":"additional","affiliation":[{"name":"Federal Institute for Health Protection of Consumers and Veterinary Medicine Diedersdorfer Weg 1 12277 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Staak","sequence":"additional","affiliation":[{"name":"Federal Institute for Health Protection of Consumers and Veterinary Medicine Diedersdorfer Weg 1 12277 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Protz","sequence":"additional","affiliation":[{"name":"Federal Institute for Health Protection of Consumers and Veterinary Medicine Diedersdorfer Weg 1 12277 Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1998,5,9]]},"reference":[{"key":"e_1_2_6_2_1","volume-title":"Techniques for the Brucellosis Laboratory","author":"ALTON G. 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