{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T15:51:21Z","timestamp":1762271481543,"version":"build-2065373602"},"reference-count":33,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[1999,5,1]],"date-time":"1999-05-01T00:00:00Z","timestamp":925516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[1999,5]]},"abstract":"<jats:p>\n                    Oestrosis is a parasitic disease of sheep and goats caused by the nasal bot fly\n                    <jats:italic>Oestrus ovis<\/jats:italic>\n                    . In the United Kingdom the economic losses as a result of infestation can be considered negligible, but the differentiation of\n                    <jats:italic>O ovis<\/jats:italic>\n                    cases from more serious diseases such as listeriosis, gid and sheep scab is of considerable importance. Currently, diagnosis of oestrosis relies on the subjective observation of clinical signs or the demonstration of larvae postmortem. This paper assesses the effectiveness of a direct enzyme\u2010linked immunosorbent assay (ELISA) using a crude somatic antigen from first\u2010stage larvae (L\n                    <jats:sub>1<\/jats:sub>\n                    ) in the serodiagnosis of oestrosis. The system has been validated with sera from both endemic and non\u2010endemic areas and the results correlated with the clinical data found postmortem. The sensitivity and specificity of the assay were 97.4 per cent and 97.6 per cent, respectively, using a cut\u2010off point based on 35 per cent binding of a reference positive control serum.\n                  <\/jats:p>","DOI":"10.1136\/vr.144.18.497","type":"journal-article","created":{"date-parts":[[2010,10,5]],"date-time":"2010-10-05T02:05:51Z","timestamp":1286244351000},"page":"497-501","source":"Crossref","is-referenced-by-count":19,"title":["Evaluation of a direct ELISA for the serodiagnosis of\n                    <i>Oestrus ovis<\/i>\n                    infections in sheep"],"prefix":"10.1002","volume":"144","author":[{"given":"P.","family":"Goddard","sequence":"first","affiliation":[{"name":"Veterinary Laboratories Agency Addlestone Surrey KT15 3NB"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Bates","sequence":"additional","affiliation":[{"name":"Veterinary Laboratories Agency Addlestone Surrey KT15 3NB"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K. A.","family":"Webster","sequence":"additional","affiliation":[{"name":"Veterinary Laboratories Agency Addlestone Surrey KT15 3NB"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1999,5]]},"reference":[{"issue":"129","key":"e_1_2_7_2_1","first-page":"173","article-title":"Update on the symptoms and treatment of Oestrus ovis infestations","volume":"22","author":"ALZIEU J. P.","year":"1990","journal-title":"Point Veterinaire"},{"volume-title":"Parasitology and Invasive Diseases of Agricultural Animals","year":"1959","author":"ANTIPIN D. 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