{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T07:52:53Z","timestamp":1779177173001,"version":"3.51.4"},"reference-count":43,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2000,4,1]],"date-time":"2000-04-01T00:00:00Z","timestamp":954547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000199","name":"U.S. Department of Agriculture","doi-asserted-by":"publisher","award":["Regional Research project NC\u201062"],"award-info":[{"award-number":["Regional Research project NC\u201062"]}],"id":[{"id":"10.13039\/100000199","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[2000,4]]},"abstract":"<jats:p>\n                    Pathogenic intestinal spirochaetes of pigs include\n                    <jats:italic>Brachyspira<\/jats:italic>\n                    (formerly\n                    <jats:italic>Serpulina<\/jats:italic>\n                    )\n                    <jats:italic>hyodysenteriae<\/jats:italic>\n                    , the cause of swine dysentery, and\n                    <jats:italic>Brachyspira pilosicoli<\/jats:italic>\n                    , the cause of porcine colonic spirochetosis (Pcs). The purpose of this study was to assess the relative importance of\n                    <jats:italic>Brachyspira<\/jats:italic>\n                    species in diarrhoeal disease of growing pigs on farms in southern Brazil. The intensity and pattern of haemolysis, the production of indole and the hydrolysis of hippurate by reference and field porcine intestinal spirochaetes were compared with 16S\u2010ribosomal RNA (rRNA)\u2010 and 23S\u2010rRNA\u2010based polymerase chain reaction assays for the identification of\n                    <jats:italic>B hyodysenteriae<\/jats:italic>\n                    and\n                    <jats:italic>B pilosicoli<\/jats:italic>\n                    . Between July and October 1998, 206 rectal swabs were taken from pigs on 17 farms with a history of diarrhoea developing within 30\u00a0days after they had been moved from nursery to growing facilities. Of 49 \u03b2\u2010haemolytic spirochaetes that were cultured, 29 (59.2 per cent) were grown in pure culture for phenotypic and genotypic characterisation, leaving 20 untyped. Of the 29 typed isolates, eight isolates obtained from six farms were identified as\n                    <jats:italic>B hyodysenteriae<\/jats:italic>\n                    , and 15 isolates obtained from seven other farms were identified as\n                    <jats:italic>B pilosicoli<\/jats:italic>\n                    ; the remaining six isolates were identified as weakly, \u03b2\u2010haemolytic commensal spirochaetes. There was complete agreement between the results of the phenotypic and genotypic analyses.\n                  <\/jats:p>","DOI":"10.1136\/vr.146.14.398","type":"journal-article","created":{"date-parts":[[2010,10,8]],"date-time":"2010-10-08T14:05:40Z","timestamp":1286546740000},"page":"398-403","source":"Crossref","is-referenced-by-count":18,"title":["Prevalence of\n                    <i>Brachyspira<\/i>\n                    species isolated from diarrhoeic pigs in Brazil"],"prefix":"10.1002","volume":"146","author":[{"given":"D. E. S. N.","family":"Barcellos","sequence":"first","affiliation":[{"name":"Instituto de Microbiologia \u2018Paulo de G\u00f3es\u2019 Universidade Federal de Rio de Janeiro Rio de Janeiro RJ Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"de Uzeda","sequence":"additional","affiliation":[{"name":"Instituto de Microbiologia \u2018Paulo de G\u00f3es\u2019 Universidade Federal de Rio de Janeiro Rio de Janeiro RJ Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. R.","family":"Mathiesen","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences University of Nebraska\u2010 Lincoln Lincoln NE 68583\u20100905 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. 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