{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T09:32:09Z","timestamp":1789205529718,"version":"build-2803163510"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2000,1,8]],"date-time":"2000-01-08T00:00:00Z","timestamp":947289600000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[2000,1]]},"abstract":"<jats:p>Thirty\u2010three pigs affected by porcine dermatitis and nephropathy syndrome, 30 from Spain and three from the USA, were investigated in order to detect porcine circovirus (Pcv) in their tissues. A standard in situ hybridisation technique using a specific DNA 317\u2010bp probe based on a well\u2010conserved sequence of Pcv (which recognises both Pcv\u2010i and PCV\u20102) was applied to formalin\u2010fixed, paraffin\u2010embedded tissues. Twentyeight of the 30 Spanish pigs and all three American pigs had Pcv in at least one tissue. Viral nucleic acid was detected mainly in lymphoid organs, and especially the lymph nodes. The viral genome was also found, in order of decreasing quantity, in Peyer's patches, tonsil, lung, spleen, kidney, liver, and skin. Viral nucleic acid was located mainly within the cytoplasm of monocyte\/macrophage lineage cells, including follicular dendritic cells, macrophages, histiocytes and Kupffer cells. No viral nucleic acid was found in damaged glomeruli or arteriolar walls. In frozen samples available from three Spanish pigs, the virus was identified as type 2 by using the polymerase chain reaction and restriction fragment length polymorphism. Most of the pigs from which serum was available were seropositive against porcine respiratory and reproductive syndrome virus (PRRSV), and PRRSV antigen was detected in the lung of two of the Spanish pigs. These results suggested that Pcv is present in tissues of almost all pigs affected by PDNS, and Pcv has to be considered as a possible agent involved in the pathogenesis of the syndrome.<\/jats:p>","DOI":"10.1136\/vr.146.2.40","type":"journal-article","created":{"date-parts":[[2010,10,8]],"date-time":"2010-10-08T14:05:40Z","timestamp":1286546740000},"page":"40-43","source":"Crossref","is-referenced-by-count":168,"title":["Identification of porcine circovirus in tissues of pigs with porcine dermatitis and nephropathy syndrome"],"prefix":"10.1002","volume":"146","author":[{"given":"C.","family":"Rosell","sequence":"first","affiliation":[{"name":"Departament de Patologia i Producci\u00f3 Animals Facultat de Veterin\u00e0ria Bellaterra Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Segal\u00e9s","sequence":"additional","affiliation":[{"name":"Departament de Patologia i Producci\u00f3 Animals Facultat de Veterin\u00e0ria Bellaterra Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. A.","family":"Ramos\u2010Vara","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathology College of Veterinary Medicine University of Missouri Columbia MO USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. M.","family":"Folch","sequence":"additional","affiliation":[{"name":"Departament de Patologia i Producci\u00f3 Animals Facultat de Veterin\u00e0ria Bellaterra Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. M.","family":"Rodriguez\u2010Arrioja","sequence":"additional","affiliation":[{"name":"Departament de Patologia i Producci\u00f3 Animals Facultat de Veterin\u00e0ria Bellaterra Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. O.","family":"Duran","sequence":"additional","affiliation":[{"name":"Department of Large Animal Clinical Sciences Veterinary Medical Center Michigan State University East Lansing MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Balasch","sequence":"additional","affiliation":[{"name":"Fort Dodge Veterinaria Vall de Bianya Girona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Plana\u2010Dur\u00e1n","sequence":"additional","affiliation":[{"name":"Fort Dodge Veterinaria Vall de Bianya Girona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Domingo","sequence":"additional","affiliation":[{"name":"Departament de Patologia i Producci\u00f3 Animals Facultat de Veterin\u00e0ria Bellaterra Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2000,1,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(94)00136-K"},{"key":"e_1_2_6_3_1","first-page":"467","article-title":"Novel porcine circoviruses from pigs with wasting disease syndrome","volume":"142","author":"ALLAN G. 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