{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T06:50:18Z","timestamp":1761634218854,"version":"build-2065373602"},"reference-count":36,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2002,11,23]],"date-time":"2002-11-23T00:00:00Z","timestamp":1038009600000},"content-version":"vor","delay-in-days":22,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007225","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","award":["1FD97\u20100435"],"award-info":[{"award-number":["1FD97\u20100435"]}],"id":[{"id":"10.13039\/100007225","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[2002,11]]},"abstract":"<jats:p>\n                    A new immunocapture technique has been applied to the diagnosis of ovine brucellosis under experimental conditions. The tests were made on a serum bank derived from both young and adult ewes vaccinated conjunctivally with the Rev 1 strain at a dose of 10\n                    <jats:sup>8<\/jats:sup>\n                    to 10\n                    <jats:sup>9<\/jats:sup>\n                    colony\u2010forming units. Adult ewes were infected experimentally two\u2010and\u2010a\u2010half years after they had been vaccinated and the results were compared with an unvaccinated control group. The condition of each animal in terms of infection with\n                    <jats:italic>Brucella melitensis<\/jats:italic>\n                    was determined by clinical and bacteriological investigations. The development of the immune response was compared by the rose bengal test, the complement fixation test, the Coombs' test and the immunocapture technique for 180\u00a0days after the vaccination and for 410\u00a0days after the experimental infection, that is, the two following gestations. The results suggest that the new technique is more specific in animals vaccinated conjunctivally, regardless of their age when they were vaccinated. After the experimental infection, significantly (P&lt;0.05) fewer of the vaccinated sheep which were free of clinical signs and were not excreting\n                    <jats:italic>B melitensis<\/jats:italic>\n                    reacted positively to the test.\n                  <\/jats:p>","DOI":"10.1136\/vr.151.21.629","type":"journal-article","created":{"date-parts":[[2010,10,5]],"date-time":"2010-10-05T02:27:44Z","timestamp":1286245664000},"page":"629-635","source":"Crossref","is-referenced-by-count":3,"title":["Evaluation of a new immunocapture test for the diagnosis of ovine brucellosis caused by Brucella melitensis"],"prefix":"10.1002","volume":"151","author":[{"given":"M.","family":"Dur\u00e1n\u2010Ferrer","sequence":"first","affiliation":[{"name":"Laboratorio Central de Veterinaria de Santa Fe Centro Nacional de Referencia de Brucelosis Animales Ministerio de Agricultura, Pesca y Alimentaci\u00f3n Camino del Jau s\/n E\u201018320 Santa Fe Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Mendoza","sequence":"additional","affiliation":[{"name":"Vircell, SL Pol\u00edgono Industrial 12 de Octubre E\u201018320 Santa Fe Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Osuna","sequence":"additional","affiliation":[{"name":"Instituto de Biotecnolog\u00eda Universidad de Granada Campus Fuentenueva E\u201018071 Granada Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Caporale","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise\u2018G. Caporale\u2019 OIE Reference Laboratory for Brucellosis Via Campo Boario 1\u201064100 Teramo Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Lucas","sequence":"additional","affiliation":[{"name":"Laboratorio Central de Veterinaria de Santa Fe Centro Nacional de Referencia de Brucelosis Animales Ministerio de Agricultura, Pesca y Alimentaci\u00f3n Camino del Jau s\/n E\u201018320 Santa Fe Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Le\u00f3n","sequence":"additional","affiliation":[{"name":"Unidad de Enfermedades Infecciosas Facultad de Veterinaria Universidad de Murcia Campus de Espinardo Murcia Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Garrido","sequence":"additional","affiliation":[{"name":"Laboratorio Central de Veterinaria de Santa Fe Centro Nacional de Referencia de Brucelosis Animales Ministerio de Agricultura, Pesca y Alimentaci\u00f3n Camino del Jau s\/n E\u201018320 Santa Fe Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,11,23]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"crossref","first-page":"54","DOI":"10.1016\/S0034-5288(18)34369-8","article-title":"Vaccination of goats with reduced doses of Rev 1 Brucella melitensis vaccine","volume":"11","author":"ALTON G. G.","year":"1970","journal-title":"Research in Veterinary Science"},{"key":"e_1_2_6_3_1","first-page":"383","volume-title":"Animal Brucellosis","author":"ALTON G. G.","year":"1990"},{"key":"e_1_2_6_4_1","first-page":"793","article-title":"Rev 1 Brucella melitensis vaccine. A review of ten years of study","volume":"37","author":"ALTON G. G.","year":"1967","journal-title":"Veterinary Bulletin"},{"key":"e_1_2_6_5_1","first-page":"13","volume-title":"Techniques for the Brucellosis Laboratory","author":"ALTON G. G.","year":"1988"},{"key":"e_1_2_6_6_1","first-page":"121","volume-title":"Las T\u00e9cnicas de Laboratorios en la Brucelosis","author":"ALTON G. 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M.","year":"1985"},{"key":"e_1_2_6_11_1","first-page":"127","volume-title":"Prevention of Brucellosis in the Mediterranean Countries","author":"CAPORALE V.","year":"1992"},{"volume-title":"COM (1999) 719 final","year":"2000","author":"COMMISSION OF EUROPEAN COMMUNITIES","key":"e_1_2_6_12_1"},{"key":"e_1_2_6_13_1","first-page":"25","volume-title":"Brucellosis: Clinical and Laboratory Aspects","author":"CORBEL M. J.","year":"1989"},{"key":"e_1_2_6_14_1","first-page":"242","volume-title":"Manual of Standards for Diagnostic Tests and Vaccines","author":"CORBEL M. J.","year":"1996"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1006\/biol.1998.0158"},{"volume-title":"Consideraciones sobre la epidemiolog\u00eda y el control de la brucelosis en peque\u00f1os rumiantes","year":"1994","author":"DE FRUTOS C.","key":"e_1_2_6_16_1"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.5.1159-1165.1994"},{"key":"e_1_2_6_18_1","unstructured":"DUR\u00c1N\u2010FERRER M.(1998)Comparaci\u00f3n entre m\u00e9todos immunol\u00f3gicos de diagn\u00f3stico de la brucelosis ovina porBrucella melitensisy eficacia de la immunizaci\u00f3n de ovejas adultas con la vacuna Rev 1 por v\u00eda conjuntival. PhD thesis University of Murcia Spain"},{"key":"e_1_2_6_19_1","first-page":"1033","article-title":"Immunisation against Brucella infection. VII. Immunological and epidemiological studies in Cordoba, Spain","volume":"20","author":"ELBERG S. S.","year":"1959","journal-title":"Bulletin of the World Health Organization"},{"key":"e_1_2_6_20_1","first-page":"67","article-title":"Rev 1 Brucella melitensis vaccine. Part II, 1968\u20101980","volume":"51","author":"ELBERG S. S.","year":"1981","journal-title":"Veterinary Bulletin"},{"key":"e_1_2_6_21_1","first-page":"1193","article-title":"Rev 1 Brucella melitensis vaccine. Part III, 1981\u20101995","volume":"66","author":"ELBERG S.","year":"1996","journal-title":"Veterinary Bulletin"},{"key":"e_1_2_6_22_1","first-page":"139","volume-title":"Brucella melitensis.","author":"FARINA R.","year":"1985"},{"key":"e_1_2_6_23_1","first-page":"295","article-title":"Comparison between subcutaneous and conjunctival route of vaccination with Rev 1 strain against Brucella melitensis infection in ewes","volume":"13","author":"FENSTERBANK R.","year":"1982","journal-title":"Annales de Recherches V\u00e9t\u00e9rinaires"},{"key":"e_1_2_6_24_1","first-page":"351","article-title":"Vaccination of ewes by a single conjunctival administration of Brucella melitensis Rev 1 vaccine","volume":"16","author":"FENSTERBANK R.","year":"1985","journal-title":"Annales de Recherches V\u00e9t\u00e9rinaires"},{"key":"e_1_2_6_25_1","first-page":"397","article-title":"Conjunctival vaccination of young goats with Brucella melitensis strain Rev 1","volume":"18","author":"FENSTERBANK R.","year":"1987","journal-title":"Annales de Recherches V\u00e9t\u00e9rinaires"},{"key":"e_1_2_6_26_1","first-page":"475","volume-title":"Manual of Standards for Diagnostic Tests and Vaccines","author":"GARIN\u2010BASTUJI B.","year":"2000"},{"key":"e_1_2_6_27_1","first-page":"255","article-title":"Brucella melitensis infection in sheep: present and future","volume":"29","author":"GARIN\u2010BASTUJI B.","year":"1998","journal-title":"Veterinary Research"},{"key":"e_1_2_6_28_1","first-page":"223","volume-title":"Prevention of Brucellosis in the Mediterranean Countries","author":"GARRIDO F.","year":"1992"},{"key":"e_1_2_6_29_1","first-page":"283","article-title":"Estudio comparativo del test Brucellacapt con el test de Coombs para Brucella","volume":"17","author":"G\u00d3MEZ M. C.","year":"1999","journal-title":"Enfermedades Infecciosas y Microbiolog\u00eda Cl\u00ednica"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(92)90117-C"},{"key":"e_1_2_6_31_1","first-page":"153","volume-title":"Animal Brucellosis","author":"MACMILLAN A.","year":"1990"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.6.2.269-272.1999"},{"key":"e_1_2_6_33_1","first-page":"41","volume-title":"Brucellosis: Clinical and Laboratory Aspects","author":"NICOLETTI P.","year":"1989"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5877(95)00513-7"},{"key":"e_1_2_6_35_1","doi-asserted-by":"crossref","first-page":"4000","DOI":"10.1128\/JCM.38.11.4000-4005.2000","article-title":"Evaluation of an immunocapture\u2010agglutination test (Brucellacapt) for the serodiagnosis of human brucellosis","volume":"38","author":"ORDU\u00d1A A.","year":"2000","journal-title":"Journal of Clinical Microbiology"},{"key":"e_1_2_6_36_1","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1128\/jcm.16.4.737-738.1982","article-title":"Microtiter adapted method that facilitates the Coombs' test for brucellosis","volume":"16","author":"OTERO J.","year":"1982","journal-title":"Journal of Clinical Microbiology"},{"key":"e_1_2_6_37_1","first-page":"92","volume-title":"Comit\u00e9 de Expertos en Patrones Biol\u00f3gicos. Serie Informes T\u00e9cnicos. 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