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These semen preparations were then tested for residual chlamydial viability by an in vitro cell culture assay, and any detrimental effect of the antibiotics on the motility and viability of the sperm was assessed. Penicillin at 25 iu\/ml or more, erythromycin at 1000 \u03bcg\/mI or more and tetracycline at 200 \u03bcg\/mI or more were highly effective at rendering the chlamydiae non\u2010viable, but streptomycin showed no antichlamydial activity. There was a significant reduction of the motility of spermatozoa extended in diluents containing erythromycin (P&amp;#60;0.05), but spermatozoa incubated with tetracycline up to concentrations of 200 \u03bcg\/mI were not affected.<\/jats:p>","DOI":"10.1136\/vr.153.19.588","type":"journal-article","created":{"date-parts":[[2010,10,8]],"date-time":"2010-10-08T13:44:05Z","timestamp":1286545445000},"page":"588-591","source":"Crossref","is-referenced-by-count":5,"title":["Evaluation of tetracycline, erythromycin, penicillin and streptomycin for decontaminating koala semen contaminated in vitro with chlamydiae"],"prefix":"10.1002","volume":"153","author":[{"given":"T. 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