{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T20:41:05Z","timestamp":1788295265452,"version":"build-2803163510"},"reference-count":0,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2005,6,4]],"date-time":"2005-06-04T00:00:00Z","timestamp":1117843200000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bmj.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[2005,6]]},"abstract":"<jats:p>\n                    An\n                    <jats:sc>ELISA<\/jats:sc>\n                    developed to diagnose\n                    <jats:italic>Fasciola hepatica<\/jats:italic>\n                    infection in cattle by detecting serum antibodies was adapted and validated for use with samples of bulk\u2010tank milk. The prevalence of the infection in 61 dairy herds was established by using serum antibody levels or faecal egg counts measured in a proportion of the cows in each herd. The correlation between the results of the\n                    <jats:sc>ELISA<\/jats:sc>\n                    and the herd seroprevalence was 0\u00b783. Using a cut\u2010off value of 27 per cent positive, the bulk\u2010tank\n                    <jats:sc>ELISA<\/jats:sc>\n                    identified herds in which more than 25 per cent of the cows were infected with a diagnostic sensitivity of 96 per cent (95 per cent confidence interval 89 to 100 per cent) and a diagnostic specificity of 80 per cent (95 per cent confidence interval 66 to 94 per cent). By applying the\n                    <jats:sc>ELISA<\/jats:sc>\n                    to 623 herds in England and 445 herds in Wales, the prevalence of\n                    <jats:italic>F hepatica<\/jats:italic>\n                    infection in England was estimated to be 48 per cent (95 per cent confidence interval 46 to 54 per cent), and in Wales 86 per cent (95 per cent confidence interval 84 to 90 per cent).\n                  <\/jats:p>","DOI":"10.1136\/vr.156.23.729","type":"journal-article","created":{"date-parts":[[2014,10,23]],"date-time":"2014-10-23T13:01:21Z","timestamp":1414069281000},"page":"729-731","update-policy":"https:\/\/doi.org\/10.1136\/crossmarkpolicy","source":"Crossref","is-referenced-by-count":63,"title":["Prevalence of\n                    <i>Fasciola hepatica<\/i>\n                    in dairy herds in England and Wales measured with an ELISA applied to bulk\u2010tank milk"],"prefix":"10.1002","volume":"156","author":[{"given":"M. R.","family":"Salimi\u2010Bejestani","sequence":"first","affiliation":[{"name":"Department of Veterinary Parasitology Liverpool School of Tropical Medicine\/Faculty of Veterinary Science Pembroke Place Liverpool L3 5QA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. G.","family":"Daniel","sequence":"additional","affiliation":[{"name":"Veterinary Laboratories Agency Job's Well Road Johnstown Carmarthen SA31 3EZ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. M.","family":"Felstead","sequence":"additional","affiliation":[{"name":"Department of Veterinary Parasitology Liverpool School of Tropical Medicine\/Faculty of Veterinary Science Pembroke Place Liverpool L3 5QA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. J.","family":"Cripps","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Science Faculty of Veterinary Science University of Liverpool Leahurst Neston Cheshire CH64 7TE"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Mahmoody","sequence":"additional","affiliation":[{"name":"Department of Veterinary Parasitology Liverpool School of Tropical Medicine\/Faculty of Veterinary Science Pembroke Place Liverpool L3 5QA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. J. L.","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of Veterinary Parasitology Liverpool School of Tropical Medicine\/Faculty of Veterinary Science Pembroke Place Liverpool L3 5QA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,6,4]]},"container-title":["Veterinary Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1136%2Fvr.156.23.729","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bvajournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1136\/vr.156.23.729","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T06:56:28Z","timestamp":1761634588000},"score":1,"resource":{"primary":{"URL":"https:\/\/bvajournals.onlinelibrary.wiley.com\/doi\/10.1136\/vr.156.23.729"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,6]]},"references-count":0,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2005,6]]}},"alternative-id":["10.1136\/vr.156.23.729"],"URL":"https:\/\/doi.org\/10.1136\/vr.156.23.729","archive":["Portico"],"relation":{},"ISSN":["0042-4900","2042-7670"],"issn-type":[{"value":"0042-4900","type":"print"},{"value":"2042-7670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,6]]}}}