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They were randomly assigned within genotype to groups and were sequentially euthanased and examined postmortem at intervals of six or 12\u00a0months, depending on their predicted susceptibility. Tissue pools prepared from the three, four or five dosed animals in each group were inoculated into groups of 20 RIII mice as a bioassay for infectivity. Separate inocula were prepared from the matched control sheep killed at each time. In the ARQ\/ARQ sheep killed four months after inoculation, infectivity was detected in the Peyer's patch tissue pool, and at 10\u00a0months it was detected in the spleen pool; from 16\u00a0months, infectivity was detected in a range of nervous and lymphoreticular tissues, including the spinal cord pool, distal ileum excluding Peyer's patches, liver, Peyer's patches, mesenteric and prescapular lymph nodes, spleen, tonsil and cervical thymus. 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