{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T14:50:12Z","timestamp":1770216612157,"version":"3.49.0"},"reference-count":0,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2007,11,24]],"date-time":"2007-11-24T00:00:00Z","timestamp":1195862400000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[2007,11]]},"abstract":"<jats:p>\n                    Fifty\u2010five isolates of\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    from septicaemic neonatal foals were used to validate five real\u2010time\n                    <jats:sc>pcr<\/jats:sc>\n                    assays targeting different known virulence factor genes: curli fibre (\n                    <jats:italic>csgD<\/jats:italic>\n                    ), ferric hydroxamate uptake (\n                    <jats:italic>fhuA<\/jats:italic>\n                    ), type 1A pilin (\n                    <jats:italic>fimA<\/jats:italic>\n                    ), aerobactin (\n                    <jats:italic>lutA<\/jats:italic>\n                    ) and yersiniabactin (\n                    <jats:italic>fyuA<\/jats:italic>\n                    ). A\n                    <jats:sc>pcr<\/jats:sc>\n                    assay targeting a universal sequence of the bacterial 16S r\n                    <jats:sc>rna<\/jats:sc>\n                    gene served as quality control. The\n                    <jats:sc>pcr<\/jats:sc>\n                    assays showed good analytical specificity and sensitivity on the basis of sequencing the\n                    <jats:sc>pcr<\/jats:sc>\n                    products, their lack of cross\u2010reactivity with non\u2010\n                    <jats:italic>E coli<\/jats:italic>\n                    organisms, high amplification efficiency and a limit of detection as low as 25\n                    <jats:italic>E coli<\/jats:italic>\n                    colony\u2010forming units. There were differences between the detection rates and amplification efficiencies for the five virulence genes. The\n                    <jats:sc>pcr<\/jats:sc>\n                    assays targeting genes\n                    <jats:italic>csgD<\/jats:italic>\n                    ,\n                    <jats:italic>fhuA<\/jats:italic>\n                    and\n                    <jats:italic>fyuA<\/jats:italic>\n                    were able to detect all 55\n                    <jats:italic>E coli<\/jats:italic>\n                    isolates, with gene\n                    <jats:italic>csgD<\/jats:italic>\n                    having the best amplification efficiency. The lowest detection rate and amplification efficiency of the\n                    <jats:italic>E coli<\/jats:italic>\n                    isolates was found for the\n                    <jats:italic>lutA<\/jats:italic>\n                    gene.\n                  <\/jats:p>","DOI":"10.1136\/vr.161.21.716","type":"journal-article","created":{"date-parts":[[2010,10,5]],"date-time":"2010-10-05T05:01:32Z","timestamp":1286254892000},"page":"716-718","source":"Crossref","is-referenced-by-count":25,"title":["Comparison of five real\u2010time\n                    <scp>pcr<\/scp>\n                    assays for detecting virulence genes in isolates of\n                    <i>Escherichia coli<\/i>\n                    from septicaemic neonatal foals"],"prefix":"10.1002","volume":"161","author":[{"given":"S.","family":"Mapes","sequence":"first","affiliation":[{"name":"Department of Medicine and Epidemiology School of Veterinary Medicine University of California 1 Shields Avenue Davis CA 95616 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. M.","family":"Rhodes","sequence":"additional","affiliation":[{"name":"Department of Medicine and Epidemiology School of Veterinary Medicine University of California 1 Shields Avenue Davis CA 95616 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. D.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Department of Medicine and Epidemiology School of Veterinary Medicine University of California 1 Shields Avenue Davis CA 95616 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. M.","family":"Leutenegger","sequence":"additional","affiliation":[{"name":"Department of Medicine and Epidemiology School of Veterinary Medicine University of California 1 Shields Avenue Davis CA 95616 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Pusterla","sequence":"additional","affiliation":[{"name":"Department of Medicine and Epidemiology School of Veterinary Medicine University of California 1 Shields Avenue Davis CA 95616 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2007,11,24]]},"container-title":["Veterinary Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1136%2Fvr.161.21.716","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bvajournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1136\/vr.161.21.716","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T05:41:18Z","timestamp":1761630078000},"score":1,"resource":{"primary":{"URL":"https:\/\/bvajournals.onlinelibrary.wiley.com\/doi\/10.1136\/vr.161.21.716"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,11]]},"references-count":0,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2007,11]]}},"alternative-id":["10.1136\/vr.161.21.716"],"URL":"https:\/\/doi.org\/10.1136\/vr.161.21.716","archive":["Portico"],"relation":{},"ISSN":["0042-4900","2042-7670"],"issn-type":[{"value":"0042-4900","type":"print"},{"value":"2042-7670","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,11]]}}}