{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T07:13:18Z","timestamp":1761635598984,"version":"build-2065373602"},"reference-count":40,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2008,5,17]],"date-time":"2008-05-17T00:00:00Z","timestamp":1210982400000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["30530590"],"award-info":[{"award-number":["30530590"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[2008,5]]},"abstract":"<jats:p>\n                    Serotypes 3 and 8 of\n                    <jats:italic>Actinobacillus pleuropneumoniae<\/jats:italic>\n                    , the aetiological agent of porcine pleuropneumonia, have been reported to predominate in the\n                    <jats:sc>uk<\/jats:sc>\n                    . Direct serotyping of isolates of the organism is typically determined by the immunological reactivity of rabbit serum to its surface polysaccharides, but the method has limitations, for example, cross\u2010reactions between serotypes 3, 6 and 8. This study describes the development of a serotype 3\u2010specific\n                    <jats:sc>pcr<\/jats:sc>\n                    , based on the capsule locus, which can be used in a multiplex format with the organism's specific gene\n                    <jats:italic>apxIV<\/jats:italic>\n                    . The\n                    <jats:sc>pcr<\/jats:sc>\n                    test was evaluated on 266 strains of\n                    <jats:italic>A pleuropneumoniae<\/jats:italic>\n                    and 121 strains of other organisms, including all the major respiratory bacterial pathogens of pigs. The test was highly specific and sensitive and should be useful for differentiating strains of serotypes 3, 6 and 8, and in seroprevalence and epidemiological surveys in regions where serotype 3 is prevalent, such as the\n                    <jats:sc>uk<\/jats:sc>\n                    .\n                  <\/jats:p>","DOI":"10.1136\/vr.162.20.648","type":"journal-article","created":{"date-parts":[[2010,10,5]],"date-time":"2010-10-05T17:36:25Z","timestamp":1286300185000},"page":"648-652","source":"Crossref","is-referenced-by-count":9,"title":["<scp>pcr<\/scp>\n                    specific for\n                    <i>Actinobacillus pleuropneumoniae<\/i>\n                    serotype 3"],"prefix":"10.1002","volume":"162","author":[{"given":"L.","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Paediatrics Imperial College London St Mary's Campus London W2 1PG"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. C. 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