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The samples were examined histologically and by immunohistochemistry for porcine circovirus type 2 (PCV\u20102). PMWS was diagnosed in two stages: first, an evaluation of the haematoxylin and eosinstained sections that identified the cases in which the characteristic PCV\u20102 cytoplasmic inclusion bodies were apparent, and secondly, a conclusive step in which immunohistochemistry was applied to confirm PMWS in the cases in which there were positive immunohistochemical results that coincided with lesions indicative of PMWS in at least one of the lymphoid and\/or lung tissues. The location of PCV\u20102\u00a0in specific lesions (cell depletion in lymphoid organs and interstitial pneumonia) confirmed PMWS in 45 of the 61 farms, 31 of which were also infected with porcine reproductive and respiratory syndrome virus. The lymphoid tissues were more reliable than the lungs for the diagnosis of PMWS, both in individual pigs and in groups of pigs, and farm diagnoses based on a group of pigs were more reliable than diagnoses based on single pigs.<\/jats:p>","DOI":"10.1136\/vr.164.17.519","type":"journal-article","created":{"date-parts":[[2010,10,5]],"date-time":"2010-10-05T17:44:04Z","timestamp":1286300644000},"page":"519-523","source":"Crossref","is-referenced-by-count":7,"title":["Application of a protocol for the diagnosis of postweaning multisystemic wasting syndrome in Italy"],"prefix":"10.1002","volume":"164","author":[{"given":"G.","family":"Sarli","sequence":"first","affiliation":[{"name":"Department of Veterinary Public Health and Animal Pathology School of Veterinary Medicine Universit\u00e0 di Bologna Via Tolara di Sopra 50, 40064, Ozzano Emilia Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Ostanello","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health and Animal Pathology School of Veterinary Medicine Universit\u00e0 di Bologna Via Tolara di Sopra 50, 40064, Ozzano Emilia Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Morandi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health and Animal Pathology School of Veterinary Medicine Universit\u00e0 di Bologna Via Tolara di Sopra 50, 40064, Ozzano Emilia Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Fusaro","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health and Animal Pathology School of Veterinary Medicine Universit\u00e0 di Bologna Via Tolara di Sopra 50, 40064, Ozzano Emilia Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Gnudi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health and Animal Pathology School of Veterinary Medicine Universit\u00e0 di Bologna Via Tolara di Sopra 50, 40064, Ozzano Emilia Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Bacci","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health and Animal Pathology School of Veterinary Medicine Universit\u00e0 di Bologna Via Tolara di Sopra 50, 40064, Ozzano Emilia Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Nigrelli","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna \u2018Bruno Libertini\u2019 (IZSLER) \u2014 Section of Mantova Strada Circonvallazione Sud 21\/A 46100 Mantova Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Alborali","sequence":"additional","affiliation":[{"name":"IZSLER \u2014 Section of Brescia Via A. 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