{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T10:40:05Z","timestamp":1766486405447,"version":"build-2065373602"},"reference-count":39,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2009,5,30]],"date-time":"2009-05-30T00:00:00Z","timestamp":1243641600000},"content-version":"vor","delay-in-days":29,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[2009,5]]},"abstract":"<jats:p>\n                    The paraffin\u2010embedded tissue (PET) blot method was used to investigate sections of the central nervous system and lymphatic tissues from 24 cases of classical scrapie and 25 cases of atypical\/Nor98 scrapie in sheep and four healthy control sheep. The PET blot detected deposits of PrP\n                    <jats:sup>Sc<\/jats:sup>\n                    in the brain tissue of all 49 sheep with scrapie but no PrP\n                    <jats:sup>Sc<\/jats:sup>\n                    labelling could be detected in the control sheep. By contrast, not all the atypical\/Nor98 scrapie cases were detectable by immunohistochemistry. The high sensitivity of the PET blot method made it possible to observe that in some atypical\/Nor98 cases, deposits of PrP\n                    <jats:sup>Sc<\/jats:sup>\n                    may be restricted to supratentorial brain structures and that the diagnosis may be missed when only testing the obex area, where deposits are common in classical scrapie, and the cerebellar structures, where deposits are considered to be common in atypical\/Nor98 cases.\n                  <\/jats:p>","DOI":"10.1136\/vr.164.22.677","type":"journal-article","created":{"date-parts":[[2010,10,5]],"date-time":"2010-10-05T17:44:04Z","timestamp":1286300644000},"page":"677-681","source":"Crossref","is-referenced-by-count":13,"title":["Detection of classical and atypical\/Nor98 scrapie by the paraffin\u2010embedded tissue blot method"],"prefix":"10.1002","volume":"164","author":[{"given":"W. M.","family":"Wemheuer","sequence":"first","affiliation":[{"name":"Prion and Dementia Research Unit Department of Neuropathology University Medical Centre, G\u00f6ttingen 37075 G\u00f6ttingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. L.","family":"Benestad","sequence":"additional","affiliation":[{"name":"Department of Pathology National Veterinary Institute Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Wrede","sequence":"additional","affiliation":[{"name":"Prion and Dementia Research Unit Department of Neuropathology University Medical Centre, G\u00f6ttingen 37075 G\u00f6ttingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. E.","family":"Wemheuer","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine Faculty for Agricultural Sciences University of G\u00f6ttingen 37075 G\u00f6tingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Brenig","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Medicine Faculty for Agricultural Sciences University of G\u00f6ttingen 37075 G\u00f6tingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Bratberg","sequence":"additional","affiliation":[{"name":"Department of Pathology National Veterinary Institute Oslo Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W. J.","family":"Schulz\u2010Schaeffer","sequence":"additional","affiliation":[{"name":"Prion and Dementia Research Unit Department of Neuropathology University Medical Centre, G\u00f6ttingen 37075 G\u00f6ttingen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,5,30]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1055"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-76-3-509"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.42.1.149"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2007056"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.153.7.202"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.160.19.665"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2003.11.017"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.79997-0"},{"volume-title":"Nor98\u2010like strain of scrapie found in Wyoming","year":"2007","author":"Cook W.","key":"e_1_2_6_10_1"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.162.16.518"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.155.22.707"},{"key":"e_1_2_6_13_1","first-page":"1","article-title":"Opinion of the Scientific Panel on Biological Hazards on the request from the European Commission on classification of atypical transmissible spongiform encephalopathy (TSE) cases in small ruminants","volume":"276","author":"EFSA","year":"2005","journal-title":"EFSA Journal"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.157.21.667-c"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.81539-0"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1177\/104063870401600611"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.81816-0"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(97)01081-0"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.158.8.280"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.82218-0"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0502296102"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.5A6892.2006"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2006046"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-004-0303-1"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.19.9320-9327.2001"},{"key":"e_1_2_6_26_1","first-page":"365","article-title":"Scrapie in sheep","volume":"5","author":"McGowan J. 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