{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T13:38:39Z","timestamp":1761572319021,"version":"build-2065373602"},"reference-count":25,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2009,6,13]],"date-time":"2009-06-13T00:00:00Z","timestamp":1244851200000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Record"],"published-print":{"date-parts":[[2009,6]]},"abstract":"<jats:p>\n                    Sixty\u2010nine vaginal swabs and 138 rectal swabs collected from 195 equids were analysed for the presence of thermotolerant bacteria, that is, bacteria surviving at 60 \u00b1 0.1\u00b0C for one hour. Thermotolerant Escherichia coli,\n                    <jats:italic>Enterobacter<\/jats:italic>\n                    species,\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    ,\n                    <jats:italic>Proteus<\/jats:italic>\n                    species and\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    species were isolated from 41, 16, nine, three and three of the 138 rectal swabs, respectively; seven of the\n                    <jats:italic>E coli<\/jats:italic>\n                    and two of the\n                    <jats:italic>Enterobacter<\/jats:italic>\n                    species isolates survived pasteurisation at 63.8 \u00b1 0.1\u00b0C for 30\u00a0minutes. All except three\n                    <jats:italic>E coli<\/jats:italic>\n                    , two\n                    <jats:italic>Enterobacter<\/jats:italic>\n                    species and one\n                    <jats:italic>Proteus<\/jats:italic>\n                    species isolate were resistant to three or more antimicrobial drugs, that is, they were multidrug resistant. Thermotolerant\n                    <jats:italic>E coli<\/jats:italic>\n                    ,\n                    <jats:italic>Enterobacter<\/jats:italic>\n                    species and\n                    <jats:italic>Proteus<\/jats:italic>\n                    species were isolated from 11, two and two of the 69 vaginal swabs, respectively, but only one isolate of\n                    <jats:italic>E coli<\/jats:italic>\n                    survived pasteurisation at 63.8 \u00b1 0.1\u00b0C for 30\u00a0minutes. All except two of the\n                    <jats:italic>E coli<\/jats:italic>\n                    isolates were multidrug resistant. None of the four thermotolerant isolates from nine soil samples collected on four of the farms where the equids were kept was pasteurisation resistant, but they were all multidrug resistant. Of the 10 pasteurisationresistant isolates, nine were multidrug resistant but none was resistant to chloramphenicol, ciprofloxacin, cotrimazine, cotrimoxazole or streptomycin. All the isolates grew at 42 \u00b1 0.1\u00b0C but none grew at 46 \u00b1 0.1\u00b0C or above. The\n                    <jats:italic>Enterobacter<\/jats:italic>\n                    isolates were more tolerant to pasteurisation than the\n                    <jats:italic>E coli<\/jats:italic>\n                    isolates, particularly during the first few minutes of exposure.\n                  <\/jats:p>","DOI":"10.1136\/vr.164.24.746","type":"journal-article","created":{"date-parts":[[2010,10,5]],"date-time":"2010-10-05T17:44:04Z","timestamp":1286300644000},"page":"746-750","source":"Crossref","is-referenced-by-count":3,"title":["Thermotolerance and multidrug resistance in bacteria isolated from equids and their environment"],"prefix":"10.1002","volume":"164","author":[{"given":"B. R.","family":"Singh","sequence":"first","affiliation":[{"name":"National Research Centre on Equines Sirsa Road Hisar Haryana 125 001 India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,6,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.8.3746-3749.1999"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1086\/502082"},{"key":"e_1_2_6_4_1","first-page":"462","article-title":"Application of thermotolerant microorganisms for biofertilizer preparation","volume":"40","author":"CHEN K. 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S.","year":"1996","journal-title":"Journal of Food Protection"},{"key":"e_1_2_6_17_1","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1099\/ijs.0.02347-0","article-title":"Lactobacillus thermotolerans sp nov, a novel thermotolerant species isolated from chicken faeces","volume":"53","author":"PIYANUCH N.","year":"2003","journal-title":"International Journal of Systematic and Evolutionary Microbiology"},{"key":"e_1_2_6_18_1","first-page":"363","article-title":"Safety and keeping quality of pasteurized milk under refrigeration","volume":"44","author":"PREJIT","year":"2007","journal-title":"Journal of Food Science and Technology"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.180.4.846-854.1998"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jevs.2007.04.011"},{"key":"e_1_2_6_21_1","first-page":"159","volume-title":"Proceedings of the 8th Indian Veterinary Congress and XV Annual Conference and IAAVR and National Symposium on \u2018Public\u2010Private\u2010Partnership in Veterinary Research and Education Sector\u2019.","author":"SINGH B. 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L.YEHK\u2010W.LINW\u2010C.CHINY\u2010M.LINC\u2010Y.(1997)Expression of a gene encoding a 16\u00b79\u00a0kDa heat shock protein Oshsp16.9 in Escherichia coli enhances thermotolerance.Proceedings of the National Academy of Sciences of the USA 94 10967\u201310972","DOI":"10.1073\/pnas.94.20.10967"}],"container-title":["Veterinary Record"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1136%2Fvr.164.24.746","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/bvajournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1136\/vr.164.24.746","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T12:29:52Z","timestamp":1761568192000},"score":1,"resource":{"primary":{"URL":"https:\/\/bvajournals.onlinelibrary.wiley.com\/doi\/10.1136\/vr.164.24.746"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,6]]},"references-count":25,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2009,6]]}},"alternative-id":["10.1136\/vr.164.24.746"],"URL":"https:\/\/doi.org\/10.1136\/vr.164.24.746","archive":["Portico"],"relation":{},"ISSN":["0042-4900","2042-7670"],"issn-type":[{"type":"print","value":"0042-4900"},{"type":"electronic","value":"2042-7670"}],"subject":[],"published":{"date-parts":[[2009,6]]}}}